| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 71,082 | T→C | 35.7% | I232I (ATT→ATC) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 71,082 | 0 | T | C | 35.7% | 112.4 / 124.4 | 115 | I232I (ATT→ATC) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (34/40); new base C (19/22); total (53/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.76e-01 | |||||||||||
Only 100 of 119 total aligned reads displayed.
GCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTT > W3110S.gb/71013‑71151 | gcgTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCAtt < 1:1996946/70‑1 (MQ=255) cgTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAg < 1:4018120/71‑1 (MQ=255) cgTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAg < 1:3102569/71‑1 (MQ=255) gTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAg < 1:4112316/70‑1 (MQ=255) gTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGt > 1:709087/1‑67 (MQ=255) gTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGt > 1:1292742/1‑67 (MQ=255) tCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGt > 1:2445340/1‑66 (MQ=255) acaTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCg < 1:3603684/70‑1 (MQ=255) acaTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCg < 1:2250602/70‑1 (MQ=255) acaTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCg < 1:1355023/70‑1 (MQ=255) acaTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGa < 1:1800215/71‑1 (MQ=255) aTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGa < 1:2327603/69‑1 (MQ=255) aTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAg < 1:3434443/71‑1 (MQ=255) tCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAg > 1:866653/1‑64 (MQ=255) tCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAgc > 1:3545592/1‑71 (MQ=255) cTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgct > 1:2257815/1‑71 (MQ=255) cTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgct > 1:1769176/1‑71 (MQ=255) tCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAgctgct < 1:762232/70‑1 (MQ=255) ccGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCttt > 1:3234357/1‑71 (MQ=255) ccGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCtt < 1:4310214/70‑1 (MQ=255) cGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCtttt < 1:102778/71‑1 (MQ=255) cGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCtttt < 1:4506225/71‑1 (MQ=255) gCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTg < 1:3014477/71‑1 (MQ=255) ccAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGa < 1:1409395/71‑1 (MQ=255) ccAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGa < 1:2165872/71‑1 (MQ=255) ccAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCtttt > 1:1622775/1‑69 (MQ=255) ccAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCtt > 1:2125372/1‑67 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAg < 1:4058644/71‑1 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAg < 1:2024390/71‑1 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAg < 1:4697015/71‑1 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAg > 1:4651632/1‑71 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAg < 1:708626/71‑1 (MQ=255) agcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGc < 1:1632781/71‑1 (MQ=255) agcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAg > 1:4004229/1‑70 (MQ=255) cagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCAc < 1:3242293/71‑1 (MQ=255) agTTAGGGATTAGCCTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCAc > 1:3475750/1‑70 (MQ=255) gTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCtt < 1:2412206/61‑1 (MQ=255) gTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTa < 1:3312942/71‑1 (MQ=255) ttAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAgc < 1:4682366/45‑2 (MQ=255) ttAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGc > 1:4468007/1‑50 (MQ=255) ttAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAgctg < 1:1761337/57‑1 (MQ=255) ttAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGa < 1:555019/64‑1 (MQ=255) ttAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTAc < 1:1176484/71‑1 (MQ=255) tAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCaccacc > 1:2756044/1‑71 (MQ=255) tAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTa > 1:3392232/1‑42 (MQ=255) tAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTa > 1:1246272/1‑42 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAg > 1:3399968/1‑63 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAg > 1:1402697/1‑63 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTa > 1:155313/1‑41 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTa > 1:600452/1‑41 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAgg > 1:1737953/1‑47 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTAccc > 1:3949372/1‑71 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGAGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAg > 1:2120508/1‑63 (MQ=255) gggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCg < 1:2832042/71‑1 (MQ=255) gggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTAccc < 1:2625250/70‑1 (MQ=255) ggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCgg < 1:2121579/71‑1 (MQ=255) ggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCgg < 1:721503/71‑1 (MQ=255) ggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCg > 1:51880/1‑70 (MQ=255) aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGTTTTTGAGCACCACCCGGAt > 1:1857821/1‑71 (MQ=255) aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGAt < 1:154906/71‑1 (MQ=255) ttAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAgc < 1:2651883/48‑1 (MQ=255) tAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGc > 1:3806726/1‑71 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGc > 1:3189060/1‑70 (MQ=255) gCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGTATGCCt < 1:612051/71‑1 (MQ=255) gCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCt < 1:4514386/71‑1 (MQ=255) cGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTa < 1:1799830/71‑1 (MQ=255) cGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTa < 1:2155027/71‑1 (MQ=255) tCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAgctgc < 1:4274428/45‑1 (MQ=255) tCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTAccc < 1:1579450/60‑1 (MQ=255) tCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATc > 1:3197556/1‑71 (MQ=255) ttAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGa > 1:2353546/1‑49 (MQ=255) ttAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCg > 1:634244/1‑59 (MQ=255) ttAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGa > 1:635932/1‑49 (MQ=255) ttAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGc > 1:1991400/1‑71 (MQ=255) tAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCtttt < 1:1241244/46‑1 (MQ=255) aaGCTGGCGCGAGGACCAACGTATCAGACAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCa > 1:769806/1‑71 (MQ=255) cTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCg > 1:2860018/1‑71 (MQ=255) gcgcGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGGCACCgtcg < 1:1335200/71‑1 (MQ=255) gcgcGAGGACCAACGCATTAGTCAGGCGAATCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgt < 1:1045546/69‑1 (MQ=255) gcgAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtcggt > 1:3673001/1‑71 (MQ=255) gAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtcggtcg > 1:832516/1‑71 (MQ=255) aGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGc < 1:1795572/71‑1 (MQ=255) aGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGc < 1:4226074/71‑1 (MQ=255) ggACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCa < 1:3268560/71‑1 (MQ=255) aCCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCg < 1:3659828/43‑1 (MQ=255) ccAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATg < 1:2048242/71‑1 (MQ=255) ccAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATg > 1:3113102/1‑71 (MQ=255) ccAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAAt < 1:274224/70‑1 (MQ=255) ccAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATg < 1:3826036/71‑1 (MQ=255) ccAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATg < 1:3697138/71‑1 (MQ=255) ccAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATg < 1:1243392/71‑1 (MQ=255) cAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGt < 1:590604/71‑1 (MQ=255) aaaCGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGt > 1:3872174/2‑71 (MQ=255) aCGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgtcggt > 1:3208432/1‑61 (MQ=255) aCGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg > 1:3432828/1‑71 (MQ=255) aCGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg > 1:2639316/1‑71 (MQ=255) aCGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg > 1:4335984/1‑71 (MQ=255) aTTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGcc > 1:4229523/1‑48 (MQ=255) ttAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGc < 1:682317/39‑1 (MQ=255) ttAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGtttt < 1:3288778/71‑1 (MQ=255) | GCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTT > W3110S.gb/71013‑71151 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |