| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 470,764 | C→A | 6.0% | T302N (ACC→AAC) | mdlB → | fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 470,764 | 0 | C | A | 6.0% | 335.0 / 3.5 | 99 | T302N (ACC→AAC) | mdlB | fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (51/42); new base A (3/3); total (54/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.02e-01 | |||||||||||
TTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACC > W3110S.gb/470695‑470833 | ttGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGAc > 1:4479112/1‑70 (MQ=255) aGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACg > 1:490249/1‑70 (MQ=255) gTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGc > 1:2926448/1‑70 (MQ=255) gTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGc > 1:1462264/1‑71 (MQ=255) gTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGc < 1:854514/71‑1 (MQ=255) tGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCaa < 1:4430217/65‑1 (MQ=255) tGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGc > 1:2608202/1‑70 (MQ=255) gCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGc < 1:5049740/62‑1 (MQ=255) cTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGa < 1:1351211/70‑1 (MQ=255) cTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGa < 1:4976851/70‑1 (MQ=255) cTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGAt < 1:411781/71‑1 (MQ=255) tGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGa < 1:4933455/69‑1 (MQ=255) gTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGAtgc > 1:2160454/1‑71 (MQ=255) tATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGAtgc > 1:4114573/1‑70 (MQ=255) tGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGAtgctgc < 1:4581115/71‑1 (MQ=255) tGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGAtgctg > 1:1157132/1‑70 (MQ=255) cGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCaa < 1:5099265/71‑1 (MQ=255) cGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCaa > 1:1123190/1‑71 (MQ=255) cGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCaa > 1:2010770/1‑71 (MQ=255) cGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCa < 1:4454499/70‑1 (MQ=255) cGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGAACACGCAACAGGCGATGCTGCaa < 1:1054835/71‑1 (MQ=255) tttATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACa < 1:5283673/71‑1 (MQ=255) tttATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGAACACGCAACAg < 1:2043066/57‑1 (MQ=255) tttATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGAACACGCAACAg < 1:1103705/57‑1 (MQ=255) tATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAgg > 1:4079815/1‑71 (MQ=255) aTCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAgg < 1:4699013/70‑1 (MQ=255) aTCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGc > 1:1809252/1‑71 (MQ=255) tCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGc > 1:159084/1‑70 (MQ=255) cAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCtg > 1:4081054/1‑71 (MQ=255) aGCTATCTTGGGCTACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATTCTGCAACAGGCtg < 1:2970163/70‑1 (MQ=255) aGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGc < 1:2432247/46‑1 (MQ=255) cTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCaa < 1:4983637/46‑1 (MQ=255) cTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCtgttg > 1:444967/1‑71 (MQ=255) tCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGc < 1:1728251/48‑1 (MQ=255) tCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCa < 1:3670446/57‑1 (MQ=255) ttGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAAc > 1:2870443/1‑42 (MQ=255) ttGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGa > 1:3172818/1‑48 (MQ=255) ttGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCt > 1:3477621/1‑71 (MQ=255) ttGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCt > 1:2222367/1‑71 (MQ=255) gggCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGttgttg < 1:449348/67‑1 (MQ=255) gggCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCt < 1:5223941/69‑1 (MQ=255) gggCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTgg > 1:3512496/1‑71 (MQ=255) ggCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGt < 1:2698200/71‑1 (MQ=255) cGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGt > 1:831828/1‑69 (MQ=255) gACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCt < 1:966068/56‑1 (MQ=255) gACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGt > 1:784388/1‑68 (MQ=255) gACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGt > 1:778063/1‑68 (MQ=255) aCTTAACGAACCATTAATCGAACTGAACACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAgc > 1:3040113/1‑71 (MQ=255) cTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGAt < 1:2059609/41‑1 (MQ=255) ttAACGAACCATTAATCGAACTGACCACGCAACAGGc < 1:3236133/37‑1 (MQ=255) ttAACGAACCATTAATCGAACTGACCACGCAACAGGCGa < 1:4376521/39‑1 (MQ=255) ttAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTg > 1:5117903/1‑63 (MQ=255) ttAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAgcgc > 1:370801/1‑71 (MQ=255) tAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGc > 1:3178794/1‑60 (MQ=255) aaCGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCgt > 1:1176945/1‑71 (MQ=255) aaCGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCgt > 1:4591758/1‑71 (MQ=255) aaCGAACCATTAATCGAACTGAACACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCgt > 1:1540814/1‑71 (MQ=255) aaCGAACCATTAATCGAACTGAACACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCgt > 1:1534663/1‑71 (MQ=255) aCGAACCATTAATCGAACTGACAACGCAACAGGCGATGCTGCAACAgg > 1:3707391/1‑48 (MQ=255) cGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCtgttg > 1:4710677/1‑53 (MQ=255) cGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCgtgt < 1:894814/71‑1 (MQ=255) cGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCgtgt > 1:1246829/1‑71 (MQ=255) gAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGc < 1:3119734/47‑1 (MQ=255) gAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGc < 1:2065642/47‑1 (MQ=255) gAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCgt < 1:4011838/68‑1 (MQ=255) aaCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGttt > 1:379027/1‑71 (MQ=255) aCCATTACTCGAACTGACCACGCAACAGGCGAtgc < 1:928656/35‑1 (MQ=255) aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAAc > 1:2242102/1‑41 (MQ=255) aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGttt > 1:2296976/1‑70 (MQ=255) aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTg < 1:2756278/71‑1 (MQ=255) aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTg < 1:3943878/71‑1 (MQ=255) ccATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGa < 1:5558/71‑1 (MQ=255) cATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGaa < 1:1538818/71‑1 (MQ=255) aTTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAAc > 1:2603304/1‑71 (MQ=255) ttAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCtgttgt > 1:4926870/1‑47 (MQ=255) ttAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACt < 1:956882/71‑1 (MQ=255) tAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGc < 1:242556/49‑1 (MQ=255) tAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACt > 1:1428828/1‑70 (MQ=255) tAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACt > 1:5016913/1‑70 (MQ=255) aaTCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTg < 1:4910033/70‑1 (MQ=255) aaTCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGa > 1:4566753/1‑71 (MQ=255) aaTCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGa > 1:1405608/1‑71 (MQ=255) aaTCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGa > 1:3666645/1‑71 (MQ=255) aTCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAAcag < 1:4283510/69‑3 (MQ=255) tCGAACTGACCACGCAACAGGCGATGCTGCAACAgg > 1:4858422/1‑36 (MQ=255) cGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGcgcg > 1:403507/1‑56 (MQ=255) cGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGa > 1:3795824/1‑63 (MQ=255) cGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGa > 1:4223125/1‑68 (MQ=255) gAACTGACCACGCAACAGGCGATGCTGCAACAGTCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATgga > 1:4057215/1‑71 (MQ=255) gAACTGACCACGCAACAGGCGATGCTGCAACAGGCtgttg > 1:2399396/1‑40 (MQ=255) gAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATgga > 1:1862179/1‑71 (MQ=255) gAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATgga < 1:2135012/71‑1 (MQ=255) aCTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATggacg > 1:2132368/1‑71 (MQ=255) cTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATggacg > 1:1364953/1‑70 (MQ=255) cTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATggacg > 1:5015088/1‑70 (MQ=255) aCCACGCATCAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGAcc < 1:1624240/71‑1 (MQ=255) aCCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATggacggac > 1:1264471/1‑70 (MQ=255) aCCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGAcc < 1:370011/71‑1 (MQ=255) aCCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGAcc < 1:2145382/71‑1 (MQ=255) aCCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGAcc < 1:4926521/71‑1 (MQ=255) | TTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACC > W3110S.gb/470695‑470833 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |