| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 547,694 | A→G | 100% | pseudogene (114/261 nt) pseudogene (114/1259 nt) |
ylbE → ylbE → |
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 547,694 | 0 | A | G | 97.9% | 156.2 / ‑4.1 | 47 | pseudogene (114/261 nt) pseudogene (114/1259 nt) | ylbE ylbE | ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (21/25); total (22/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGG > W3110S.gb/547630‑547759 | gtcgCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaa < 1:5295621/70‑1 (MQ=255) tcgCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaaa < 1:2558096/71‑1 (MQ=255) tcgCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaaa < 1:21143/71‑1 (MQ=255) gcgcTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAac < 1:2566496/70‑1 (MQ=255) gcgcTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAac < 1:2518529/70‑1 (MQ=255) gcgcTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAac < 1:1965163/70‑1 (MQ=255) tCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGc > 1:650211/1‑71 (MQ=255) tCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTc < 1:1141049/71‑1 (MQ=255) ctggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCg < 1:3177983/71‑1 (MQ=255) gctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgc > 1:3310127/1‑71 (MQ=255) ggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAacac > 1:3973091/1‑53 (MQ=255) gATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc < 1:5193464/71‑1 (MQ=255) aTGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc > 1:2782924/1‑70 (MQ=255) cAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATgc < 1:5061814/70‑1 (MQ=255) aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaa < 1:852879/39‑1 (MQ=255) cGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTgg > 1:1720299/1‑71 (MQ=255) gCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAacac > 1:4867370/1‑40 (MQ=255) ttcttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAg > 1:1891320/1‑70 (MQ=255) tcttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCt > 1:1751406/1‑64 (MQ=255) cttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCt < 1:2224935/40‑1 (MQ=255) ttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGc < 1:4368080/38‑1 (MQ=255) cACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGgag < 1:4511973/68‑1 (MQ=255) cACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGAt < 1:1310209/70‑1 (MQ=255) cACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGAt < 1:4562903/70‑1 (MQ=255) cACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGAt < 1:21369/70‑1 (MQ=255) cACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAgg > 1:1011501/1‑66 (MQ=255) cTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgc < 1:747492/46‑1 (MQ=255) tCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGa > 1:3630765/1‑71 (MQ=255) cAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGag > 1:1367672/1‑70 (MQ=255) aGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAg < 1:4020426/57‑1 (MQ=255) gCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc < 1:4199646/43‑1 (MQ=255) cGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGgaga > 1:3802117/1‑59 (MQ=255) cGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCa > 1:3616885/1‑71 (MQ=255) cGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCa > 1:3283599/1‑71 (MQ=255) aaCTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc < 1:240727/40‑1 (MQ=255) aaCTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc < 1:1876800/40‑1 (MQ=255) aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATgc > 1:4794668/1‑44 (MQ=255) aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATg > 1:5351643/1‑70 (MQ=255) aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGa < 1:4236773/71‑1 (MQ=255) aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgg > 1:3948795/1‑71 (MQ=255) aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgg < 1:3621835/71‑1 (MQ=255) aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgg > 1:128883/1‑71 (MQ=255) aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgg < 1:931419/71‑1 (MQ=255) aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAg > 1:615834/1‑70 (MQ=255) aCGAGGGCAAACCACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGCCCCATGa > 1:2443469/1‑66 (MQ=255) aCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGa > 1:4871109/1‑66 (MQ=255) cGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGctct > 1:2424362/1‑41 (MQ=37) | GTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGG > W3110S.gb/547630‑547759 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |