| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,162,829 | C→T | 5.3% | I769I (ATC→ATT) | mdtC → | multidrug efflux system, subunit C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,162,829 | 0 | C | T | 5.3% | 249.1 / 3.0 | 75 | I769I (ATC→ATT) | mdtC | multidrug efflux system, subunit C |
| Reads supporting (aligned to +/- strand): ref base C (41/30); new base T (2/2); total (43/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01 | |||||||||||
TAAAGTGGTGATGGAAGTGGATCCGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCTAAATGGCAACCGGCGAATGCCCCACTATC > W3110S.gb/2162760‑2162897 | taAAGTGGTGATGGAAGTGGATCCGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATc > 1:2569614/1‑70 (MQ=255) aaaGTGGTGATGGAAGTGGATCCGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCaa > 1:1720863/1‑71 (MQ=255) aaaGTGGTGATGGAAGTGGATCCGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCaa > 1:1714709/1‑71 (MQ=255) aGTGGTGATGGAAGTGGATCCGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATa < 1:5225105/71‑1 (MQ=255) tggtgATGGAAGTGGATCCGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAAt < 1:1978889/68‑1 (MQ=255) tggtgATGGAAGTGGATCCGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAAc < 1:1218762/71‑1 (MQ=255) gtgATGGAAGTGGATCCGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACg > 1:4313002/1‑70 (MQ=255) gtgATGGAAGTGGATCCGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGa < 1:620887/71‑1 (MQ=255) gATGGAAGTGGATCCGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATaa > 1:3382983/1‑66 (MQ=255) gATGGAAGTGGATCCGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAg > 1:436807/1‑71 (MQ=255) aTGGAAGTGGATCCGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAg > 1:3747713/1‑70 (MQ=255) aTGGAAGTGGATCCGCGCTATACCCAGCACATCAGTGCGCTGGAAAAAATGTTCGTTATTGATAACGaac < 1:3898653/70‑2 (MQ=255) tGGAAGTGGATCCGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAAt < 1:4708468/62‑1 (MQ=255) tGGAAGTGGATCCGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGc < 1:3084773/71‑1 (MQ=255) gAAGTGGATCCGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCaa < 1:3462851/71‑1 (MQ=255) gAAGTGGATCCGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCa < 1:3350998/70‑1 (MQ=255) aGTGGATCCGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAg > 1:4924729/1‑71 (MQ=255) gTGGATCCGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAg < 1:2373207/70‑1 (MQ=255) gTGGATCCGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGc < 1:4605936/71‑1 (MQ=255) gATCCGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGa < 1:2288222/70‑1 (MQ=255) gATCCGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGa < 1:4296879/70‑1 (MQ=255) gATCCGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGAt > 1:1251498/1‑71 (MQ=255) aTCCGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATc > 1:2899428/1‑71 (MQ=255) tCCGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATcc < 1:1224351/71‑1 (MQ=255) tCCGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATcc > 1:36431/1‑71 (MQ=255) ccGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGaa > 1:472006/1‑57 (MQ=255) ccGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAACGTTCGTTATTGATAACGa < 1:1105268/56‑1 (MQ=38) cgcgCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATccc > 1:5317251/1‑70 (MQ=255) gcgcTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGa < 1:4661339/65‑1 (MQ=255) tataCCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGa > 1:3940139/1‑50 (MQ=255) tataCCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTc > 1:2321946/1‑71 (MQ=255) cccAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTa < 1:3376774/70‑1 (MQ=255) ccAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTAt > 1:3421701/1‑70 (MQ=255) aGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGa > 1:3337355/1‑54 (MQ=255) ggACATCAGTGCGCTGGAAAAAATGTTCGTTATCAAt > 1:4811976/1‑37 (MQ=255) gACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGa < 1:914384/41‑1 (MQ=255) gACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGc > 1:2294852/1‑71 (MQ=255) gACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGc > 1:17121/1‑71 (MQ=255) aCATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGc > 1:1548358/1‑70 (MQ=255) aCATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCt < 1:1450677/71‑1 (MQ=255) cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGc > 1:3408861/1‑69 (MQ=255) cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCTa < 1:2229944/71‑1 (MQ=255) aTCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCTa > 1:1006748/1‑70 (MQ=255) tCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCg < 1:2788383/59‑1 (MQ=255) cAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTa > 1:1803594/1‑60 (MQ=255) aGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCTAAATg < 1:3088014/71‑1 (MQ=255) aGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCTAAATg < 1:3034621/71‑1 (MQ=255) gTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGa > 1:4507051/1‑45 (MQ=255) tGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCTAAATGGc < 1:4209332/71‑1 (MQ=255) tccTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCTAAATGGCaa < 1:2949687/69‑1 (MQ=255) cgcTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCTa > 1:980291/1‑63 (MQ=255) cgcTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCTAAATGGCa < 1:2351592/70‑1 (MQ=255) gcTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCTAAATgg > 1:4202595/1‑67 (MQ=255) cTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCTaa < 1:4312687/62‑1 (MQ=255) cTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCTAAATGGCAAcc < 1:4652555/71‑1 (MQ=255) gAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCTAAATGGCAACCGGc > 1:2474849/1‑71 (MQ=255) gAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGAT‑CCGCTGTCGTATTTCGCTAAATGGCAACCgg < 1:1695828/69‑1 (MQ=255) aaaaaaTGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCTAAATGGCAACCGGCg < 1:402436/71‑1 (MQ=255) aaaaaaTGTTCGTTATCAATAACGAAGGCAAAGCGATCCCCCTGTCGTATTTCGCTAAATGGCAACCGGCg < 1:3978463/71‑1 (MQ=255) aaaaaTGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCTa > 1:319561/1‑56 (MQ=255) aaaaaTGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCTAAATGGCAACCGGCg > 1:2545589/1‑70 (MQ=255) aaaaaTGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCTAAATGGCAACCGGCGa < 1:1029931/71‑1 (MQ=255) aaaTGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCg > 1:3175013/1‑44 (MQ=255) aaaTGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCg > 1:3168971/1‑44 (MQ=255) aaTGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCTAAATGGCAACCGGCGAATg > 1:4781202/1‑71 (MQ=255) aTGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCTAAATGGCAACCGGCGAATGc > 1:1513641/1‑71 (MQ=255) tGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCg > 1:382184/1‑48 (MQ=255) tGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCTAAATGGCaa > 1:437828/1‑59 (MQ=255) ttCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCTAAATGGCAACCGGCGAATGccc > 1:2883438/1‑70 (MQ=255) tCGTTATTGATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCTAAATGGCAACCGGCGAATGCCCCa > 1:4871153/1‑71 (MQ=255) tCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCTAAATGGCAACCGGCGAATGc > 1:1830558/1‑67 (MQ=255) cGTTATTGATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCTCTaaa > 1:424677/1‑49 (MQ=25) tATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTAttt > 1:3078757/1‑39 (MQ=255) tATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCTAAATGGCAACCGGCGAATGCCCCACTAt > 1:1584351/1‑71 (MQ=255) aTCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCTAAATGGCAACCGGCGAATGCCCCACTATc > 1:3527223/1‑71 (MQ=255) aTCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCTAAATGGCAACCGGCGAATGCCCCACTATc > 1:4237422/1‑71 (MQ=255) | TAAAGTGGTGATGGAAGTGGATCCGCGCTATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGTATTTCGCTAAATGGCAACCGGCGAATGCCCCACTATC > W3110S.gb/2162760‑2162897 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |