Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,627,679 C→A 5.1% P20T (CCA→ACA)  rhtB → neutral amino‑acid efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,627,6790CA 5.1% 394.5 / 3.3 115P20T (CCA→ACA) rhtBneutral amino‑acid efflux system
Reads supporting (aligned to +/- strand):  ref base C (71/38);  new base A (3/3);  total (74/41)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 126 total aligned reads displayed.

CCGGGAGTTCATCATGACCTTAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAACCACGGTTATCGCGGCGCGGTGGCGTCTAT  >  W3110S.gb/3627609‑3627749
                                                                      |                                                                      
ccGGGAGTTCATCATGACCTTAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGc                                                                        <  1:3299346/71‑1 (MQ=255)
 cGGGAGTTCATCATGACCTTAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGcc                                                                       <  1:2315040/71‑1 (MQ=255)
 cGGGAGTTCATCATGACCTTAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGc                                                                        >  1:3898749/1‑70 (MQ=255)
 cGGGAGTTCATCATGACCTTAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGc                                                                        >  1:1810108/1‑70 (MQ=255)
     aGTTCATCATGACCTTAGAATGGTGGTTTGCCTACATGCTGACATCGATCATTTTAAGCCTGTCGCCAGGc                                                                   >  1:1139648/1‑71 (MQ=255)
      gTTCATCATGACCTTAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGct                                                                  >  1:1080948/1‑71 (MQ=255)
       ttCATCATGACCTTAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGcc                                                                       <  1:4240075/65‑1 (MQ=255)
       ttCATCATGACCTTAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGcc                                                                       <  1:5136023/65‑1 (MQ=255)
         catcatGACCTTAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGct                                                                  >  1:4264444/1‑68 (MQ=255)
         catcatGACCTTAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTg                                                               >  1:4779329/1‑71 (MQ=255)
         catcatGACCTTAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTg                                                               >  1:4023975/1‑71 (MQ=255)
         catcatGACCTTAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTg                                                               >  1:545268/1‑71 (MQ=255)
         catcatGACCTTAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGGCGACAGGctta                                                                <  1:1775971/70‑3 (MQ=255)
          atcatGACCTTAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGctct                                                                <  1:4286200/69‑1 (MQ=255)
          atcatGACCTTAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTgg                                                              >  1:3807054/1‑71 (MQ=255)
            catGACCTTAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTg                                                            >  1:4612239/1‑71 (MQ=255)
              tGACCTTAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGc                                                           >  1:3993524/1‑70 (MQ=255)
               gACCTTAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCaa                                                         >  1:4001970/1‑71 (MQ=255)
                aCCTTAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCaa                                                         >  1:4712481/1‑70 (MQ=255)
                aCCTTAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCaa                                                         <  1:2342498/70‑1 (MQ=255)
                 ccTTAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAAt                                                        >  1:162169/1‑70 (MQ=255)
                 ccTTAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAAt                                                        >  1:4653030/1‑70 (MQ=255)
                 ccTTAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATc                                                       <  1:4705102/71‑1 (MQ=255)
                   ttAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAAt                                                        <  1:1627125/68‑1 (MQ=255)
                    tAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCaa                                                     >  1:4356465/1‑70 (MQ=255)
                    tAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCaa                                                     >  1:875843/1‑70 (MQ=255)
                    tAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCa                                                      >  1:4345653/1‑69 (MQ=255)
                     aGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCa                                                                      >  1:107774/1‑52 (MQ=255)
                     aGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAaca                                                   >  1:1331851/1‑71 (MQ=255)
                      gAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAacac                                                  <  1:562715/71‑1 (MQ=255)
                       aaTGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAacac                                                  <  1:5124411/70‑1 (MQ=255)
                        aTGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTa                                                <  1:1599744/71‑1 (MQ=255)
                         tggtggTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTa                                                >  1:745585/1‑70 (MQ=255)
                         tggtggTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTAt                                               <  1:3909679/71‑1 (MQ=255)
                           gtggtTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCaa                                                         <  1:4675099/59‑1 (MQ=255)
                           gtggtTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATg                                              >  1:2963610/1‑70 (MQ=255)
                            tggtTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCa                                                          >  1:1752638/1‑57 (MQ=255)
                            tggtTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGac                                            <  1:2682297/71‑1 (MQ=255)
                              gtTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGacc                                           >  1:1687045/1‑70 (MQ=255)
                               tttGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGcc                                                                       <  1:2403062/41‑1 (MQ=255)
                                ttGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATg                                              <  1:1128070/65‑1 (MQ=255)
                                 tGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCt                                       >  1:4106927/1‑71 (MQ=255)
                                 tGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCt                                       >  1:2016471/1‑71 (MQ=255)
                                  gCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCaa                                                         >  1:2035567/1‑52 (MQ=255)
                                  gCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCt                                       <  1:5159727/70‑1 (MQ=255)
                                   ccTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTAt                                               >  1:4901763/1‑61 (MQ=255)
                                      aCCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTgg                                                              >  1:4591989/1‑43 (MQ=255)
                                      aCCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTAt                                               <  1:658435/58‑1 (MQ=255)
                                      aCCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACGTCGCt                                   >  1:5210276/1‑70 (MQ=255)
                                      aCCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCt                                   >  1:4470084/1‑70 (MQ=255)
                                      aCCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCt                                   >  1:933722/1‑70 (MQ=255)
                                      aCCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCt                                   >  1:341007/1‑70 (MQ=255)
                                      aCCTGCTGACATCGATCATTTTAAGCCTCTCGCCAGGCTCTgg                                                              >  1:4464174/1‑43 (MQ=255)
                                       ccTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTc                                  >  1:3630545/1‑70 (MQ=255)
                                       ccTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTc                                  >  1:1511059/1‑70 (MQ=255)
                                       ccTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTc                                  <  1:1563952/70‑1 (MQ=255)
                                        ctgctgACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGaccacc                                        >  1:3029691/1‑63 (MQ=255)
                                        ctgctgACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCaa                                >  1:2326762/1‑71 (MQ=255)
                                         tgctgACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGc                                                           <  1:5337997/43‑1 (MQ=255)
                                          gctgACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGc                                                           <  1:3573124/42‑1 (MQ=255)
                                          gctgACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCaac                                                        >  1:341868/1‑44 (MQ=255)
                                          gctgACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAacac                                                  >  1:120816/1‑51 (MQ=255)
                                          gctgACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAAc                               >  1:3660407/1‑70 (MQ=255)
                                          gctgACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAAc                               >  1:1990726/1‑70 (MQ=255)
                                           ctgACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCa                                 <  1:1836578/67‑1 (MQ=255)
                                           ctgACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAAc                               <  1:1721726/69‑1 (MQ=255)
                                           ctgACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAACCa                             >  1:1538319/1‑71 (MQ=255)
                                           ctgACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAACCa                             >  1:2257744/1‑71 (MQ=255)
                                            tgACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATg                                              >  1:3322666/1‑53 (MQ=255)
                                            tgACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGaccgcc                                        >  1:3326011/1‑59 (MQ=255)
                                            tgACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAACCAc                            >  1:556631/1‑71 (MQ=255)
                                                aTCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAACCACGGtt                        <  1:85041/71‑1 (MQ=255)
                                                aTCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAACCACGGtt                        <  1:2430077/71‑1 (MQ=255)
                                                  cGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGaccac                                         >  1:2772606/1‑52 (MQ=255)
                                                  cGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGa                                             >  1:1432489/1‑48 (MQ=255)
                                                  cGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAACCACGGTTAt                      >  1:3543925/1‑71 (MQ=255)
                                                    aTCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAACCACGGTTATcg                    <  1:4802220/71‑1 (MQ=255)
                                                     tCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGacca                                          >  1:4452911/1‑48 (MQ=255)
                                                     tCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAACCACGGTTATcgc                   >  1:4981451/1‑71 (MQ=255)
                                                      cATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATg                                              >  1:1118242/1‑43 (MQ=255)
                                                      cATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAACCACg                           >  1:4579338/1‑62 (MQ=255)
                                                         tttAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAACCACGGTTATcgcggc                >  1:1056501/1‑70 (MQ=255)
                                                          ttAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAACCACGGTTATcgcggcgc              >  1:3584940/1‑71 (MQ=255)
                                                          ttAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAACCACGGTTATcgcggcgc              >  1:2466128/1‑71 (MQ=255)
                                                            gaGCCTGGCGACAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAACCACGGtt                        <  1:5019849/58‑1 (MQ=39)
                                                            gaGCCTGGCGACAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAACCACGGTTATcgcggcgcgg            >  1:4484456/2‑71 (MQ=255)
                                                             aGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAACCACGGTTATcgcggcgcgg            <  1:2422218/70‑1 (MQ=255)
                                                             aGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAACCACGGTTATCGCGGCGCGGt           >  1:3242827/1‑71 (MQ=255)
                                                              gCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAACCACGGTTATCGCGGCGCGGTg          <  1:768858/71‑1 (MQ=255)
                                                               ccTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAACCACGGt                         <  1:124797/55‑1 (MQ=255)
                                                                cTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAACCACGGtt                        <  1:5247385/55‑1 (MQ=255)
                                                                 tGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAACCACGGTTATCGCGGCGCGGTGGCg       >  1:2553357/1‑71 (MQ=255)
                                                                 tGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAACCACGGTTATCGCGGCGCGGTGGCg       >  1:588330/1‑71 (MQ=255)
                                                                 tGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCACAACCACGGTTATCGCGGCGCGGTGGCg       >  1:820954/1‑71 (MQ=255)
                                                                  gTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAAcc                              <  1:3369856/47‑1 (MQ=255)
                                                                  gTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAACCACGGTTATCGCGGCGCGGTGGc        >  1:3817729/1‑69 (MQ=255)
                                                                   tCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAACCACGGTTATCGCGGCGCGGTGGCGTc     <  1:3439558/71‑1 (MQ=255)
                                                                      ccAGGCTCTGGTGCAATCAACACTATGACCACCTCGc                                    <  1:1402208/37‑1 (MQ=255)
                                                                      ccAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAACCACGGTTATCGCGGCGCGGTGGCGTCTa   <  1:1427497/70‑1 (MQ=255)
                                                                      ccAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAACCACGGTTATCGCGGCGCGGTGGCGTCTAt  <  1:3150710/71‑1 (MQ=255)
                                                                      |                                                                      
CCGGGAGTTCATCATGACCTTAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACACTATGACCACCTCGCTCAACCACGGTTATCGCGGCGCGGTGGCGTCTAT  >  W3110S.gb/3627609‑3627749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: