| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,866,202 | T→C | 5.5% | intergenic (‑209/+21) | mtlD ← / ← mtlA | mannitol‑1‑phosphate dehydrogenase, NAD(P)‑binding/fused mannitol‑specific PTS enzyme IIABC components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,866,202 | 0 | T | C | 5.5% | 257.2 / 5.0 | 91 | intergenic (‑209/+21) | mtlD/mtlA | mannitol‑1‑phosphate dehydrogenase, NAD(P)‑binding/fused mannitol‑specific PTS enzyme IIABC components |
| Reads supporting (aligned to +/- strand): ref base T (35/51); new base C (3/2); total (38/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACAAATTGTGATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCTGGTGGTG > W3110S.gb/3866133‑3866271 | aacaaATTGTGATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCAt < 1:3588521/70‑1 (MQ=255) caaATTGTGATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCAtgtg < 1:1200245/71‑1 (MQ=255) caaATTGTGATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCACACCTTCTCCAtgtg > 1:1312947/1‑71 (MQ=255) aTTGTGATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCAtgtg > 1:4292489/1‑68 (MQ=255) aTTGTGATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCAtgtg > 1:838799/1‑68 (MQ=255) ttGTGATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGgaga < 1:3645101/71‑1 (MQ=255) gtgATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGagag < 1:1332377/70‑1 (MQ=255) tgATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAggg < 1:2966907/71‑1 (MQ=255) tgATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAggg > 1:3778970/1‑71 (MQ=255) tgATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAgg < 1:2653601/70‑1 (MQ=255) tgATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAgg < 1:1952358/70‑1 (MQ=255) aTGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGt < 1:2345324/70‑1 (MQ=255) aTGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGt < 1:5082437/70‑1 (MQ=255) aTGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTg > 1:2532348/1‑71 (MQ=255) cATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTg > 1:1215358/1‑68 (MQ=255) cATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGgga < 1:3630135/71‑1 (MQ=255) tCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCAtgt < 1:1160235/56‑1 (MQ=255) tCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGggatt < 1:2979297/71‑1 (MQ=255) cTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGggattg < 1:144184/70‑1 (MQ=255) tGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGggattg < 1:4112772/69‑1 (MQ=255) gCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTgg < 1:3275491/50‑1 (MQ=255) gCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGAttact < 1:3174406/71‑1 (MQ=255) gCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGAttact < 1:4312243/71‑1 (MQ=255) gCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGAttact < 1:5236795/71‑1 (MQ=255) ccTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGAttact < 1:827125/70‑1 (MQ=255) tGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGagag < 1:3803544/51‑1 (MQ=255) tGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGAttacttac > 1:680712/1‑71 (MQ=255) tGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGAtta > 1:2467193/1‑66 (MQ=255) gATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTTCATGTGGAGAGGGTGGGATTGGAttacttac > 1:4207061/1‑70 (MQ=255) gATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCAt < 1:973503/42‑1 (MQ=255) gATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGgaga > 1:1454356/1‑49 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACg < 1:3487071/70‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGa > 1:2330691/1‑71 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGa > 1:1068446/1‑71 (MQ=255) tAAGCGTAGCGCATCAGGCAATTAACCACACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGAc > 1:4852193/1‑71 (MQ=255) aGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGgaga > 1:5347440/1‑45 (MQ=255) aGCGTAGCGCATCAGGCAATTAACCCCACCCTCTCCACATGgagaa < 1:1879473/46‑2 (MQ=21) gCGTAGCGCATCAGGCAATTAACCCCACCCTCTCCACATGGAGAagg < 1:347914/47‑1 (MQ=18) cGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATTTGGAGAGGGTGggattgga > 1:3617350/1‑56 (MQ=255) cGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGc < 1:4021157/71‑1 (MQ=255) gTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGAttact < 1:2238873/60‑1 (MQ=255) gTAGCGCATCAGGCAATTAACCCCACCCTCTCCACATGGAGAAGGTg > 1:2077999/1‑47 (MQ=17) gcgcATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGggattg > 1:1271706/1‑50 (MQ=255) gcgcATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCcag > 1:4445413/1‑70 (MQ=255) cgcATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCcagc < 1:3026443/70‑1 (MQ=255) cATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGt > 1:4387983/1‑71 (MQ=255) cATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGt > 1:216850/1‑71 (MQ=255) aTCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGt < 1:2471884/39‑1 (MQ=255) aTCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGtt > 1:4544558/1‑71 (MQ=255) aTCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGt < 1:375539/70‑1 (MQ=255) aGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTcc < 1:3100337/70‑1 (MQ=255) aGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTc > 1:2038205/1‑69 (MQ=255) ggCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCcagca > 1:4469543/1‑64 (MQ=255) ggCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAg < 1:1964857/71‑1 (MQ=255) gCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGATCTGCCAGCAGTTCCAg > 1:2510022/1‑70 (MQ=255) gCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAg > 1:4504366/1‑70 (MQ=255) cAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCa < 1:2791445/71‑1 (MQ=255) aaTTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCcagc < 1:101380/60‑1 (MQ=255) tAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGAttactta > 1:1445527/1‑45 (MQ=255) tAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACtt < 1:4414191/70‑1 (MQ=255) aaCCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCa > 1:141234/1‑71 (MQ=255) aCCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCa < 1:591826/70‑1 (MQ=255) tcccACCCTCTCCACATGGAGAAGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATc > 1:1312819/2‑71 (MQ=25) ccccACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATc > 1:2937224/1‑71 (MQ=255) ccccACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATc < 1:2218806/71‑1 (MQ=255) cccACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATc > 1:3572972/1‑70 (MQ=255) ccACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGt < 1:4366777/55‑1 (MQ=255) ccACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTc < 1:1991114/66‑1 (MQ=255) cACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCAc < 1:3502840/71‑1 (MQ=255) aCCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCAc > 1:3682374/1‑70 (MQ=255) aCCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACg > 1:1283496/1‑71 (MQ=255) ccTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCcag < 1:2719237/48‑1 (MQ=255) ccTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTcc > 1:1005395/1‑55 (MQ=255) ccTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGc < 1:1727949/71‑1 (MQ=255) cTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGc < 1:2420796/57‑1 (MQ=255) cTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACt < 1:1174665/60‑1 (MQ=255) cTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCt > 1:1874980/1‑71 (MQ=255) cTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCt < 1:4943847/71‑1 (MQ=255) cTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCt < 1:4450701/71‑1 (MQ=255) cTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCt < 1:1681338/71‑1 (MQ=255) ttCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGAc < 1:1412/39‑1 (MQ=255) ttCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCtg > 1:4335628/1‑71 (MQ=255) ctcCATGTGGAGAGGGTGGGATTGGATTACTTACGACCt > 1:5070236/1‑39 (MQ=255) ctcCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCa > 1:642705/1‑52 (MQ=255) ctcCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACt > 1:4896151/1‑57 (MQ=255) ctcCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCtgg < 1:104236/70‑1 (MQ=255) ctcCACATGGAGAAGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCtggt > 1:5330517/1‑71 (MQ=39) ccATGTGGAGAGGGTGGGATTGGATTACTTACGAcc < 1:4380401/36‑1 (MQ=255) cATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCATCACTTc < 1:691583/56‑1 (MQ=255) cATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCtggtg < 1:5333411/69‑1 (MQ=255) cATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCtggtg < 1:3677309/69‑1 (MQ=255) cATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCtggtg < 1:225612/69‑1 (MQ=255) aTGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCcagca < 1:1019998/42‑1 (MQ=255) aTGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCtggtgg > 1:1285971/1‑69 (MQ=255) aTGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCTGgtggtg < 1:1831368/71‑1 (MQ=255) aTGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCTGgtggtg < 1:793763/71‑1 (MQ=255) aTGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCTGgtggtg < 1:1783070/71‑1 (MQ=255) aTGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCTGgtggtg > 1:5066698/1‑71 (MQ=255) tgtgGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTc < 1:2070336/45‑1 (MQ=255) | AACAAATTGTGATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCTGGTGGTG > W3110S.gb/3866133‑3866271 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |