| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 71,109 | T→C | 33.3% | T241T (ACT→ACC) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 71,109 | 0 | T | C | 33.3% | 144.2 / 117.3 | 120 | T241T (ACT→ACC) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (42/38); new base C (23/17); total (65/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 123 total aligned reads displayed.
AGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGT > W3110S.gb/71039‑71177 | agTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACt < 1:2502356/71‑1 (MQ=255) gTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTa < 1:2430911/71‑1 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTAcc < 1:5275718/70‑1 (MQ=255) gggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCg < 1:4241191/71‑1 (MQ=255) gATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTGAGCACCACCCg < 1:488479/68‑1 (MQ=255) aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGAt > 1:466457/1‑71 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGcc > 1:192436/1‑71 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGc < 1:1656966/70‑1 (MQ=255) gCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACt < 1:4344936/59‑1 (MQ=255) cGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTa < 1:3915731/71‑1 (MQ=255) gTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAG‑‑GAAGCTGCTTTTGAGCACCACCCGGAt > 1:1816525/1‑63 (MQ=255) gTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTAt < 1:2342374/71‑1 (MQ=255) cTTAAGCTGGCGCGAGGACCAACGTATCATCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTa < 1:715658/68‑1 (MQ=255) ttAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGGACTACCCGGATGCCTATCGc > 1:1330258/1‑71 (MQ=255) ttAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATg < 1:912031/63‑1 (MQ=255) ttAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGc > 1:1690825/1‑71 (MQ=255) ttAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGc > 1:4481425/1‑71 (MQ=255) aaGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCAACACCCGGATGCCTATCGcc < 1:1655581/70‑1 (MQ=255) aaGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTAcc > 1:2075380/1‑55 (MQ=255) aaGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCa > 1:4746701/1‑71 (MQ=255) aGCTGGCGCGAGGACCAACGTATCAGCCAGGGGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCAc > 1:4505672/1‑71 (MQ=255) aGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCa < 1:3113384/70‑1 (MQ=255) aGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCa < 1:2739823/70‑1 (MQ=255) cTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCg < 1:88186/71‑1 (MQ=255) tGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgt > 1:2387668/1‑71 (MQ=255) ggCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtc > 1:818990/1‑71 (MQ=255) gcgcGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgt < 1:5359410/69‑1 (MQ=255) gcgcGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtcg < 1:2458902/71‑1 (MQ=255) gcgcGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtcg > 1:4286323/1‑71 (MQ=255) gcgcGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtcg > 1:3260800/1‑71 (MQ=255) cgcgAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGcaccac > 1:4164977/1‑47 (MQ=255) gcgAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtc > 1:2207400/1‑68 (MQ=255) aGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCg < 1:3335859/46‑1 (MQ=255) aGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGc < 1:3763957/71‑1 (MQ=255) aGGACCAACGCATTAGTCAGGCAAAGCTGCTTTTGAGCACTACCCGGATGCCTAt > 1:3243357/1‑55 (MQ=255) ggACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCa > 1:5099008/1‑59 (MQ=255) aCCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAAt < 1:3548641/71‑1 (MQ=255) aCCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAAt < 1:3113784/71‑1 (MQ=255) ccAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATg < 1:787452/71‑1 (MQ=255) cAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCgg < 1:4376902/42‑1 (MQ=25) cAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgt > 1:1826983/1‑59 (MQ=255) aCGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgtcggtc > 1:1966203/1‑62 (MQ=255) aCGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg > 1:454551/1‑71 (MQ=255) aCGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg > 1:1038653/1‑71 (MQ=255) aTTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGttt < 1:1583411/71‑1 (MQ=255) aTTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGtt < 1:2433849/70‑1 (MQ=255) ttAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGtttt < 1:228956/71‑1 (MQ=255) ttAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGtttt < 1:3775578/71‑1 (MQ=255) aGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtcggtc > 1:4168042/1‑55 (MQ=255) aGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGa > 1:1924926/1‑71 (MQ=255) gTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGa < 1:5016675/70‑1 (MQ=255) gTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGAc < 1:794383/71‑1 (MQ=255) tCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTgg < 1:1588532/63‑1 (MQ=255) ccAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACg < 1:2390371/70‑1 (MQ=255) cAGGCGAAGCTGCTTTTGAGCACCCCCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg > 1:4566216/1‑61 (MQ=255) aGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGa > 1:3030306/1‑67 (MQ=255) aGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGa < 1:2010990/70‑1 (MQ=255) ggCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATc < 1:3465636/71‑1 (MQ=255) ggCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGAt > 1:1129468/1‑70 (MQ=255) gCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGtttt > 1:1071245/1‑63 (MQ=255) gCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATc > 1:2396806/1‑70 (MQ=255) gCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCaa > 1:4174916/1‑53 (MQ=255) gCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg < 1:2621729/58‑1 (MQ=255) gCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGAt > 1:1876479/1‑69 (MQ=255) gCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCa < 1:4764918/71‑1 (MQ=255) gCGAAGCTGCTTTTGAGCACCACCCGGATCCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATc > 1:4741879/1‑70 (MQ=255) cGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCAc > 1:3527893/1‑40 (MQ=255) cGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCTGTCGCaa > 1:3327911/1‑52 (MQ=255) cGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCaa > 1:510171/1‑71 (MQ=255) gAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAAc < 1:4120679/71‑1 (MQ=255) gAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAAc > 1:381171/1‑71 (MQ=255) gAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAAc > 1:4614730/1‑71 (MQ=255) aGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATg > 1:3976381/1‑51 (MQ=255) aGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATg > 1:3969978/1‑51 (MQ=255) gctgctTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAActct < 1:3561393/71‑1 (MQ=255) gctgctTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAActct < 1:1546235/71‑1 (MQ=255) tgctTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCaa > 1:1739441/1‑65 (MQ=255) tgctTTTGAGCACTA‑‑CGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTAttt > 1:1900510/1‑71 (MQ=255) gctTTTGAGCACCACCCGGATTCCTATCGCCACCGTCGGTCGCAATGTTGGtt > 1:2742900/1‑53 (MQ=255) gctTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGa > 1:54067/1‑57 (MQ=255) gctTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAActct < 1:4677213/68‑1 (MQ=255) ctTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTAttt > 1:4601797/1‑71 (MQ=255) ctTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTAttt > 1:2859816/1‑71 (MQ=255) ctTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTAt < 1:1355141/69‑1 (MQ=255) ctTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTAt < 1:3960805/69‑1 (MQ=255) ctTTTGAGCACCACCCGGATGCCTATCGCCACCgtcgg > 1:4769173/1‑38 (MQ=255) ctTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGa > 1:3538115/1‑56 (MQ=255) ttttGAGCACCACCCGGATGCCTATCGCCACCgtcg > 1:87616/1‑36 (MQ=255) ttttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATc > 1:568903/1‑60 (MQ=255) ttttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCa > 1:4845958/1‑61 (MQ=255) tttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGtt > 1:1037608/1‑46 (MQ=255) ttGAGCACTACCCGGATGCCTATCGCCACCGTCGGTTGCAATGTTGGtttt < 1:4674787/51‑1 (MQ=255) ttGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTtct > 1:4171588/1‑70 (MQ=255) ttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGt > 1:1276113/1‑44 (MQ=255) aGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTcgc < 1:4925425/70‑1 (MQ=255) aGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTcgc > 1:3178261/1‑70 (MQ=255) aGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTcgc < 1:2408354/70‑1 (MQ=255) gCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTcgcgg > 1:3701493/1‑70 (MQ=255) cACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAg > 1:1853943/1‑71 (MQ=255) aCTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGt < 1:1168361/71‑1 (MQ=255) | AGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGT > W3110S.gb/71039‑71177 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |