| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 981,683 | Δ1 bp | 33.3% | coding (11/533 nt) | ydfQ ← | predicted lysozyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 981,683 | 0 | C | . | 33.3% | 50.4 / 23.0 | 24 | coding (11/533 nt) | ydfQ | predicted lysozyme |
| Reads supporting (aligned to +/- strand): ref base C (7/9); new base . (5/3); total (12/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01 | |||||||||||
GTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCACCCGCCCGCTTCGTCTTACGATATTCATCATAAACTTTGGGATCATACTGAAGCT > minE/981616‑981749 | gTATCTGAGGAGCAGATGCTCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATtttt < 1:3648653/71‑1 (MQ=255)gTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATtttt > 1:3251480/1‑71 (MQ=255) aTCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATttttg < 1:938423/70‑2 (MQ=255) tCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGAT‑TTTtgt > 1:984255/1‑70 (MQ=255) tCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGAT‑TTTtgt > 1:5252930/1‑70 (MQ=255) tGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGAT‑TTTTGTGtt > 1:207669/1‑71 (MQ=255) aggagCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGAT‑TTTTGTGTTc < 1:4797198/70‑1 (MQ=255) aggagCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGAT‑TTTTGTGTTCa > 1:5373979/1‑71 (MQ=255) gagCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATttttg > 1:2617870/1‑62 (MQ=255) gagCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATttttg > 1:2996416/1‑62 (MQ=255) gcgcCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGAT‑TTTtgtg > 1:2555640/1‑57 (MQ=255) cgcCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCACCCGCCCGCt > 1:1070278/1‑71 (MQ=255) cAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGAT‑TTTTGTGTTCa < 1:4332644/57‑1 (MQ=255) cAGCACCAATCAGAGCCAGAACGGCAGCCGACAGGCCGTATCTGAT‑TTTTGTGtt < 1:4041225/55‑1 (MQ=255) gCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATttttg > 1:4892089/1‑48 (MQ=255) aCCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCACCCGCCCGCTTCGTCt > 1:1143069/1‑70 (MQ=255) aaTCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCACCCGCCCGCTTCGTCTTAc > 1:3255114/1‑70 (MQ=255) aaTCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCACCCGCCCGCTTCGTCTTAc > 1:5145841/1‑70 (MQ=255) tCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCACCCGCCCGCTTCGTCTTAc < 1:3082781/68‑1 (MQ=255) cgcCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCACCCGCCCGCTTCGTCTTACGATATtc < 1:297876/71‑1 (MQ=255) gcCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTtg < 1:2838583/38‑1 (MQ=255) aaCGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCa > 1:892158/1‑39 (MQ=255) cGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCACccgcccgc > 1:2273123/1‑46 (MQ=255) cGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCACccgcccgc > 1:959034/1‑46 (MQ=255) ccGACAGGCCGTATCTGATCTTTTGTGTTCACCCGCCCGCTTCGTCTTACGATATTCATCATAAACTTTgg < 1:2635366/71‑1 (MQ=255) aGGCCGTATCTGATCTTTTGTGTTCACCCGCCCGCTTCGTCTTACGATATTCATCATAAACTTTGGGATCa < 1:1152428/71‑1 (MQ=255) aGGCCGTATCTGATCTTTTGTGTTCACCCGCCCGCTTCGTCTTACGATATTCATCATAAACTTTGGGATCa < 1:785446/71‑1 (MQ=255) gCCGTATCTGATCTTTTGTGTTCACCCGCCCGCTTCGTCTTACGa < 1:748868/45‑1 (MQ=255) gCCGTATCTGATCTTTTGTGTTCACCCGCCCGCTTCGTCTTACGa < 1:2446459/45‑1 (MQ=255) aTCTTTTGTGTTCACCCGCCCGCTTCGTCTTACGATATTCATCATAAACTTTGGGATCATACTGAAGCt < 1:1288189/69‑1 (MQ=255) cTTTTGTGTTCACCCGCCCGCTTCGTCTTACGATATTCATCATAAACt > 1:2735436/1‑48 (MQ=255) | GTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCACCCGCCCGCTTCGTCTTACGATATTCATCATAAACTTTGGGATCATACTGAAGCT > minE/981616‑981749 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |