| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,467,882 | Δ1 bp | 28.6% | intergenic (+41/+87) | ubiG → / ← yfaL | bifunctional 3‑demethylubiquinone‑9 3‑methyltransferase and 2‑octaprenyl‑6‑hydroxy phenol methylase/adhesin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,467,882 | 0 | C | . | 28.6% | 112.9 / 43.5 | 49 | intergenic (+41/+87) | ubiG/yfaL | bifunctional 3‑demethylubiquinone‑9 3‑methyltransferase and 2‑octaprenyl‑6‑hydroxy phenol methylase/adhesin |
| Reads supporting (aligned to +/- strand): ref base C (18/16); new base . (7/7); total (26/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01 | |||||||||||
AACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGC > minE/1467812‑1467946 | aaCTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGc > 1:1855911/1‑71 (MQ=255) tatGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCcgc < 1:1572228/69‑1 (MQ=255) tatGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGt > 1:3275972/1‑71 (MQ=255) tgctgcACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCt < 1:4200223/71‑1 (MQ=255) acacGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATTCCTGATGCGACGCTTGCCGCGTCTTATCAg < 1:1863481/71‑1 (MQ=255) acacGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTAt > 1:4636442/1‑68 (MQ=255) acacGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAg < 1:2071342/71‑1 (MQ=255) gAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc > 1:72546/1‑70 (MQ=255) aTAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACaa > 1:3396830/1‑70 (MQ=255) tAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTa < 1:135197/57‑1 (MQ=255) tAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTa < 1:2119619/57‑1 (MQ=255) gTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCt < 1:1940781/71‑1 (MQ=255) gAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTAt > 1:1451398/1‑53 (MQ=255) gTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTcc‑‑cc < 1:843942/70‑1 (MQ=255) tGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGGCGCGTCt > 1:2197221/1‑45 (MQ=255) tGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCt > 1:1664298/1‑45 (MQ=255) tGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGt > 1:2447871/1‑70 (MQ=255) tGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGc < 1:2023096/71‑1 (MQ=255) tGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGc > 1:3144410/1‑71 (MQ=255) tGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGa > 1:3473915/1‑71 (MQ=255) cgcgCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAg < 1:468940/71‑1 (MQ=255) cgcgCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAg < 1:3733454/71‑1 (MQ=255) cgcCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAGGc < 1:150269/71‑1 (MQ=255) ccAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAGGCGt > 1:5322616/1‑71 (MQ=255) ccAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAGGCGt > 1:1534616/1‑71 (MQ=255) cAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAGGCGtt < 1:1838129/71‑1 (MQ=255) cAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAGGCGtt < 1:647468/71‑1 (MQ=255) aaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAGGCGtt > 1:125423/1‑70 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGt > 1:5569488/1‑49 (MQ=255) tGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCg < 1:2097987/46‑1 (MQ=255) aTGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCa > 1:2453231/1‑71 (MQ=255) aTGCGACGCTTG‑CGCGTCTTATCAGGCCTACAAATTCCCGCACCCTCCGTAGGCCGGATAAGGCGTTTAcg < 1:5374231/71‑1 (MQ=0) aTGCGACGCTTG‑CGCGTCTTATCAGGCCTACAAATTCCCGCACCCTCCGTAGGCCGGATAAGGCGTTTAcg > 1:4890722/1‑71 (MQ=0) aTGCGACGCTTG‑CGCGTCTTATCAGGCCTACAAATTCCCGCACCCTCCGTAGGCCGGATAAGGCGTTTAcg > 1:4963371/1‑71 (MQ=0) aTGCGACGCTTG‑CGCGTCTTATCAGGCCTACAAATTCCCGCACCCTCCGTAGGCCGGATAAGGCGTTTAc < 1:4719260/70‑1 (MQ=0) tGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCg > 1:37603/1‑43 (MQ=255) tGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCg > 1:1700515/1‑43 (MQ=255) tGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCa < 1:4427823/70‑1 (MQ=255) tGCGACGCTTG‑CGCGTCTTATCAGGCCTACAAATTCCCGCACCCTCCGTAGGCCGGATAAGGCGTTTAcgc > 1:1345902/1‑71 (MQ=0) gCGACGCTTG‑CGCGTCTTATCAGGCCTACAAATTCCCGCACCATCCGTAGGCCGGATAAGGCGTTTAcgcc > 1:29656/1‑71 (MQ=0) gCGACGCTTG‑CGCGTCTTATCAGGCCTACAAATTCCCGCACCATCCGTAGGCCGGATAAGGCGTTTAcgcc > 1:4485858/1‑71 (MQ=0) cGACGCTTG‑CGCGTCTTATCAGGCCTACAAATTCCCGCACCATCCGTAGGCCGGATAAGGCGTTTAcgcc < 1:4567717/70‑1 (MQ=0) aCGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg > 1:1858644/1‑71 (MQ=255) aCGCTTG‑CGCGTCTTATCAGGCCTACAAATTCCCGCACCCTCCGTAGGCCGGATAAGGCGTTTACGCCGCa < 1:3533806/71‑1 (MQ=0) aCGCTTG‑CGCGTCTTATCAGGCCTACAAATTCCCGCACCCTCCGTAGGCCGGATAAGGCGTTTACGCCGCa > 1:1204526/1‑71 (MQ=0) aCGCTTG‑CGCGTCTTATCAGGCCTACAAATTCCCGCACCCTCCGTAGGCCGGATAAGGCGTTTACGCCGCa > 1:3608400/1‑71 (MQ=0) cGCTTG‑CGCGTCTTATCAGGCCTACAAATTCCCGCACCCTCCGTAGGCCGGATAAGGCGTTTACGCCGCAt < 1:5289091/71‑1 (MQ=0) cGCTTG‑CGCGTCTTATCAGGCCTACAAATTCCCGCACCCTCCGTAGGCCGGATAAGGCGTTTACGCCGCAt < 1:4720923/71‑1 (MQ=0) gCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc > 1:3375370/1‑70 (MQ=255) gCTTG‑CGCGTCTTATCAGGCCTACAAATTCCCGCACCATCCGTAGGCCGGATAAGGCGTTTACGCCGCATc < 1:445947/71‑1 (MQ=0) | AACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGC > minE/1467812‑1467946 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |