| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,019,219:1 | +C | 26.5% | intergenic (+168/+58) | ydgA → / ← uidC | conserved hypothetical protein/predicted outer membrane porin protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,019,219 | 1 | . | C | 26.5% | 34.3 / 42.8 | 34 | intergenic (+168/+58) | ydgA/uidC | conserved hypothetical protein/predicted outer membrane porin protein |
| Reads supporting (aligned to +/- strand): ref base . (12/13); new base C (4/5); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑ACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTT > minE/1019150‑1019277 | cgacgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCAtaaaaac < 1:274881‑M2/26‑2 (MQ=255) gacgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCataaaaaca > 1:1857692‑M2/45‑69 (MQ=255) cTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑Ac < 1:1111320/71‑1 (MQ=255) cTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑Ac < 1:5511288/71‑1 (MQ=255) acgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGcataaaaacac < 1:2096420‑M2/28‑4 (MQ=255) acgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATaaaaa < 1:1281470‑M2/25‑1 (MQ=255) acgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATaaaaa < 1:3098024‑M2/25‑1 (MQ=255) ctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATAAAAACAcgcg < 1:4858968‑M2/31‑1 (MQ=255) ctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATAAAAACAcgcg < 1:3770558‑M2/31‑1 (MQ=255) acacTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑ACGCGCa > 1:1026075/1‑71 (MQ=255) cacTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑ACGCGCAc < 1:829182/71‑1 (MQ=255) aagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATAAAAACACGCGCa < 1:4692444‑M2/33‑1 (MQ=255) aagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATAAAAACACGCGCAc > 1:774384‑M2/38‑71 (MQ=255) agcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATAAAAACACGCGCAc < 1:3150977‑M2/34‑1 (MQ=255) tttGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑ACGCGCACttt > 1:3941050/1‑71 (MQ=255) gcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATAAAAACACGCGCACtt > 1:1562269‑M2/36‑71 (MQ=255) tGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑ACGCGCACTTTg < 1:1034573/70‑1 (MQ=255) cttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATAAAAACACGCGCACTTTg > 1:4855398‑M2/32‑69 (MQ=255) ttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATAAAAACACGCGCACtttgcta > 1:5651377‑M2/31‑68 (MQ=255) tATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑ACGCGCACTTTGTcaacaa > 1:4326407/1‑71 (MQ=255) aGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑ACGCGCACtt < 1:687423/58‑1 (MQ=255) aGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑ACGCGCACTTTGTCAACAATCt > 1:5362425/1‑70 (MQ=255) gTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑ACGCGCACTTTGTCAACAATCTGa < 1:5690720/71‑1 (MQ=255) tttttGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑ACGCGCACTTTg < 1:3932357/58‑1 (MQ=255) catagcactgcaacgtatGTTTACACCGCATCCGGCataaaaaca > 1:194876‑M2/19‑43 (MQ=255) tagcactgccacgtatGTTTACACCGCATCCGGCATaaaaa > 1:4103816‑M2/17‑41 (MQ=255) agcactgccacgtatGTTTACACCGCATCCGGCATaaaaa > 1:1438687‑M2/16‑40 (MQ=255) agcactgccacgtatGTTTACACCGCATCCGGCATaaaaa > 1:462448‑M2/16‑40 (MQ=255) agcactgccacgtatGTTTACACCGCATCCGGCATaaaaa > 1:1712395‑M2/16‑40 (MQ=255) agcactgccacgtatGTTTACACCGCATCCGGCATaaaaa > 1:781077‑M2/16‑40 (MQ=255) gcactgccacgtatGTTTACACCGCATCCGGCataaaaaca > 1:1837029‑M2/15‑39 (MQ=255) gcactgccacgtatGTTTACACCGCATCCGGCataaaaaca > 1:2988740‑M2/15‑39 (MQ=255) ctgccacgtatGTTTACACCGCATCCGGCATAAAAACACGCGCACTTtgc < 1:2442155‑M2/39‑2 (MQ=255) gATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑ACGCGCACTTTGTCAACAATCTGAAACGCCGGAGAttt > 1:4011657/1‑71 (MQ=255) aGGCGTTTACGCCGCATCCGTCATAAAAA‑ACGCGCACTTTGTCAACAATCTGa < 1:5426096/53‑1 (MQ=255) ggCGTTTACGCCGCATCCGGCATAAAAA‑ACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTtctc < 1:1972496/71‑1 (MQ=255) ttACGCCGCATCCGGCATAAAAA‑ACGCGCACTTTGTCAACAATCTGaa < 1:4598763/48‑1 (MQ=255) tACGCCGCATCCGGCATAAACA‑ACGCGCACTTTGTCAGCAAt > 1:1310397/1‑42 (MQ=11) tACGCCGCATCCGGCATAAACA‑ACGCGCACTTTGTCAGCAATCTGa > 1:1292796/1‑46 (MQ=11) tACGCCGCATCCGGCATAAACA‑ACGCGCACTTTGTCAGCAATCTGa > 1:1228585/1‑46 (MQ=11) tACGCCGCATCCGGCATAAACA‑ACGCGCACTTTGTCAGCAATCTGa > 1:1513414/1‑46 (MQ=11) tACGCCGCATCCGGCATAAACA‑ACGCGCACTTTGTCAGCAATCTGa > 1:222252/1‑46 (MQ=11) gccgcATCCGGCATAAAAA‑ACGCGCACTTTGTCAACAATCt < 1:1631437/41‑1 (MQ=255) tCCGGCATAAAAA‑ACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTtc < 1:5319841/54‑1 (MQ=255) tCCGGCATAAAAA‑ACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTAttttt < 1:278421/71‑1 (MQ=255) ccGGCATAAAAA‑ACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTAttttt > 1:3570536/1‑70 (MQ=255) ggCATAAAAA‑ACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTtct > 1:1639613/1‑52 (MQ=255) aa‑aCGCGAACTTTGTCAACAATCTGAAACGCCGGAGAttt < 1:2456921/40‑1 (MQ=255) a‑aCGCGCACTTTGTCAACAATCTGAAACGCCGgaga < 1:2993764/36‑1 (MQ=255) a‑aCGAGCACTTTGTCAACAATCTGAAACGCCGgaga < 1:2436101/36‑1 (MQ=255) | CCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑ACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTT > minE/1019150‑1019277 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |