| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,823,325:1 | +G | 17.5% | intergenic (+62/+66) | norW → / ← hypF | NADH:flavorubredoxin oxidoreductase/carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,823,325 | 1 | . | G | 17.5% | 175.0 / 45.8 | 57 | intergenic (+62/+66) | norW/hypF | NADH:flavorubredoxin oxidoreductase/carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases |
| Reads supporting (aligned to +/- strand): ref base . (20/27); new base G (4/6); total (24/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01 | |||||||||||
GATGTAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGT > minE/1823257‑1823389 | gATGTAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑Cg < 1:2565379/71‑1 (MQ=255)gATGTAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑Cg < 1:5339693/71‑1 (MQ=255) aTGTAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGc < 1:3050926/71‑1 (MQ=255) aTGTAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGc < 1:5402383/71‑1 (MQ=255) gTAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCAt > 1:555788/1‑71 (MQ=255) tAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCAtg > 1:5614669/1‑71 (MQ=255) ggTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCAtgtg < 1:2626366/71‑1 (MQ=255) gTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATgtgt < 1:1008766/71‑1 (MQ=255) gTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATgtgt < 1:5467348/71‑1 (MQ=255) ggCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAgg > 1:3997628/1‑71 (MQ=255) ggCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAgg < 1:2687919/71‑1 (MQ=255) cTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGc > 1:3974633/1‑67 (MQ=255) cTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGa < 1:2086222/71‑1 (MQ=255) tgCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGAt < 1:60408/69‑1 (MQ=255) tgCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATaa < 1:2136262/71‑1 (MQ=255) gCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAg > 1:5442851/1‑71 (MQ=255) cTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCAtgt < 1:4765372/55‑1 (MQ=255) cTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGc < 1:61392/71‑1 (MQ=255) cTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGc < 1:839346/71‑1 (MQ=255) aaaaTGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATgtgt < 1:4665323/55‑1 (MQ=255) gggtGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGc < 1:3300383/66‑1 (MQ=18) gggtGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCg < 1:5224143/67‑1 (MQ=18) aaaTGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGtt < 1:2659720/71‑1 (MQ=255) aaaTGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGtt > 1:3545806/1‑71 (MQ=255) aaaTGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGt < 1:5583964/70‑1 (MQ=255) ggtGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGtt < 1:2093581/69‑1 (MQ=21) aTGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTa > 1:3678413/1‑71 (MQ=255) tGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTAc > 1:4195416/1‑71 (MQ=255) tCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTAcg < 1:226097/70‑1 (MQ=255) tCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTAcg < 1:1206975/70‑1 (MQ=255) cGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTAcgcc > 1:1009605/1‑71 (MQ=255) ggATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTAcgcc < 1:1886594/70‑1 (MQ=255) gATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTAcgccg < 1:3502571/71‑1 (MQ=18) gATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTAcgccg < 1:2220199/71‑1 (MQ=18) aTGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCg > 1:106473/1‑51 (MQ=255) gCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCAt < 1:342817/70‑1 (MQ=255) cGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATc < 1:5260542/70‑1 (MQ=255) cGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATc < 1:2446669/70‑1 (MQ=255) gACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATcc > 1:4586736/1‑71 (MQ=18) gTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTc > 1:3734995/1‑71 (MQ=255) gTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTc > 1:4468053/1‑71 (MQ=255) ttATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAt > 1:3773046/1‑62 (MQ=255) ttATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAt > 1:2486958/1‑62 (MQ=255) tCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATg > 1:1056081/1‑71 (MQ=255) ccGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGgtgt > 1:381741/1‑63 (MQ=255) cGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGtt < 1:521248/40‑1 (MQ=17) cGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGc < 1:2103605/70‑1 (MQ=255) cGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGc < 1:1676696/70‑1 (MQ=255) cGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGCGTCCAAATGc < 1:150420/70‑1 (MQ=255) ccTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTa > 1:170489/1‑39 (MQ=14) cTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAacac > 1:3485102/1‑71 (MQ=255) aCGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTAcgcc > 1:2492745/1‑40 (MQ=11) aCGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTAcgcc > 1:617926/1‑40 (MQ=11) aCGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGt > 1:1425518/1‑71 (MQ=255) cGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc > 1:4126872/1‑47 (MQ=255) cGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc > 1:1035101/1‑47 (MQ=255) cGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACg < 1:4216816/69‑1 (MQ=255) | GATGTAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGT > minE/1823257‑1823389 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |