Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,303,892:1 +C 10.0% intergenic (+65/‑119) metJ → / → yiiX DNA‑binding transcriptional repressor, S‑adenosylmethionine‑binding/predicted peptidoglycan peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,303,8921.C10.0% 226.7 / 24.0 60intergenic (+65/‑119)metJ/yiiXDNA‑binding transcriptional repressor, S‑adenosylmethionine‑binding/predicted peptidoglycan peptidase
Reads supporting (aligned to +/- strand):  ref base . (26/28);  new base C (4/2);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

AAGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAA  >  minE/2303827‑2303961
                                                                  |                                                                      
aaGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCa                                                                   <  1:2753124/70‑1 (MQ=255)
  gCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAAtt                                                                >  1:2785279/1‑71 (MQ=255)
    aaaaaaGAGCGGCGGGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTa                                                              <  1:1439509/71‑1 (MQ=255)
      aaaaGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTAtt                                                            >  1:560992/1‑71 (MQ=255)
       aaaGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTAtt                                                            >  1:446652/1‑70 (MQ=255)
       aaaGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTg                                                           <  1:456896/71‑1 (MQ=255)
         agagCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTg                                                           <  1:3538734/69‑1 (MQ=255)
         agagCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTg                                                           <  1:2476149/69‑1 (MQ=255)
          gagCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAAt                                                        <  1:3732742/71‑1 (MQ=255)
                  cgGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTa                                                              <  1:3937039/57‑1 (MQ=255)
                      gTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACtt                                            <  1:4268655/71‑1 (MQ=255)
                       tGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAAATTATTGAATTTGCACGAACTTg                                           <  1:197399/71‑1 (MQ=255)
                         gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTg                                                     <  1:1978944/59‑1 (MQ=255)
                         gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGt                                          <  1:1434517/70‑1 (MQ=255)
                         gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGt                                          <  1:4594440/70‑1 (MQ=255)
                         gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGt                                          <  1:843407/70‑1 (MQ=255)
                         gAATCGCCTGATGCGCTACGCTTATCAGACCTACGTCATATTGCAATTTa                                                              >  1:674553/1‑50 (MQ=255)
                          aaTCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAAt                                                        <  1:4816554/55‑1 (MQ=255)
                          aaTCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTg                                           <  1:4278153/68‑1 (MQ=255)
                            tCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGa                                                          <  1:1657780/51‑1 (MQ=255)
                            tCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTACTGAATTTg                                                     >  1:327091/1‑56 (MQ=255)
                              gCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGc                                      >  1:4704022/1‑69 (MQ=255)
                                 tGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAAtt                                                                <  1:3939240/40‑1 (MQ=255)
                                 tGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTg                                                           <  1:4051071/45‑1 (MQ=255)
                                   aTGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTa                                                              >  1:4396494/1‑40 (MQ=255)
                                   aTGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt                                 <  1:157414/69‑1 (MQ=255)
                                   aTGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt                                 <  1:5246844/69‑1 (MQ=255)
                                   aTGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt                                 >  1:3972239/1‑69 (MQ=255)
                                   aTGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt                                 <  1:2440714/69‑1 (MQ=255)
                                   aTGCGCTACGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGa                                  >  1:3907292/1‑70 (MQ=2)
                                    tGCGCTACGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGAt                                 >  1:3803956/1‑70 (MQ=2)
                                    tGCGCTACGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGAt                                 >  1:3459869/1‑70 (MQ=2)
                                       gcTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCg                           >  1:3102765/1‑71 (MQ=255)
                                        cTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGc                                      <  1:3405734/59‑1 (MQ=255)
                                         tACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGtt                         >  1:5184782/1‑71 (MQ=255)
                                         tACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGtt                         >  1:4047786/1‑71 (MQ=255)
                                          aCGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGtt                         >  1:3891404/1‑70 (MQ=255)
                                           cGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAGGCGtt                         <  1:3029182/71‑1 (MQ=2)
                                            gCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTa                                         >  1:3582468/1‑52 (MQ=255)
                                             cTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATa                                >  1:5691889/1‑60 (MQ=255)
                                              ttATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAGGCGtt                         <  1:303607/68‑1 (MQ=2)
                                                aTCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCAcgccg                  >  1:3580256/1‑71 (MQ=255)
                                                aTCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAGGCGTTCAcgc                    >  1:3866421/1‑71 (MQ=2)
                                                   aGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAgg                                       >  1:4349288/1‑47 (MQ=255)
                                                   aGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt                                 <  1:3365622/53‑1 (MQ=255)
                                                   aGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCAt               <  1:5221789/71‑1 (MQ=255)
                                                    ggCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATc              <  1:3792219/71‑1 (MQ=255)
                                                     gCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATcc             >  1:3514639/1‑71 (MQ=255)
                                                     gCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATc              <  1:2469343/70‑1 (MQ=255)
                                                       cTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGt                          >  1:426077/1‑56 (MQ=255)
                                                       cTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg           <  1:5416851/71‑1 (MQ=255)
                                                         aCGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGa                                  <  1:2987731/46‑1 (MQ=255)
                                                          cGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATaa                               >  1:3843765/1‑48 (MQ=255)
                                                          cGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATaa                               >  1:2546639/1‑48 (MQ=255)
                                                          cGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa         >  1:3740576/1‑70 (MQ=255)
                                                          cGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa         >  1:4573581/1‑70 (MQ=255)
                                                          cGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa         >  1:3871214/1‑70 (MQ=255)
                                                           gTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCa                       >  1:3675332/1‑55 (MQ=255)
                                                            tCATATTGCAATTTATTGAATTTGCACGAACTTGTa                                         >  1:2644667/1‑36 (MQ=255)
                                                            tCATATTGCAATTTATTGAATTTGCACGAACTTGTa                                         >  1:188142/1‑36 (MQ=255)
                                                              atatTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaac    <  1:4141506/71‑1 (MQ=255)
                                                               tatTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaaca   <  1:856903/71‑1 (MQ=255)
                                                                atTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaacaa  >  1:2532159/1‑71 (MQ=255)
                                                                atTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaacaa  >  1:2453031/1‑71 (MQ=255)
                                                                  |                                                                      
AAGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAA  >  minE/2303827‑2303961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: