| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,925,766:2 | +C | 25.2% | intergenic (‑102/+58) | holC ← / ← pepA | DNA polymerase III, chi subunit/aminopeptidase A, a cyteinylglycinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,925,766 | 2 | . | C | 25.2% | 200.3 / 142.9 | 111 | intergenic (‑102/+58) | holC/pepA | DNA polymerase III, chi subunit/aminopeptidase A, a cyteinylglycinase |
| Reads supporting (aligned to +/- strand): ref base . (42/41); new base C (18/10); total (60/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.42e-01 | |||||||||||
Only 100 of 113 total aligned reads displayed.
AGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCG > minE/2925701‑2925833 | aGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTa > 1:1670600/1‑69 (MQ=255)aGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAgg > 1:3317544/1‑71 (MQ=255)aGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAgg > 1:5562827/1‑71 (MQ=255)aGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAgg > 1:3846808/1‑71 (MQ=255) ataCACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCg > 1:1273586/1‑70 (MQ=255) taCACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGa < 1:210658/71‑1 (MQ=255) taCACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGa < 1:767328/71‑1 (MQ=255) cacCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGAt < 1:1314499/70‑1 (MQ=255) cacCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGAt > 1:5343328/1‑70 (MQ=255) cacCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGAt > 1:1518991/1‑70 (MQ=255) gCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGc < 1:428961/71‑1 (MQ=255) cAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCg < 1:3880629/71‑1 (MQ=255) cAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCg < 1:5350910/71‑1 (MQ=255) gctggaattgcctgatgcgctacgcttatcaggcctacgAGTTCAGTG‑‑CTCTACGTAGGCCGGATAAGGCg < 1:423301‑M2/32‑1 (MQ=255) gcgcCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGtt < 1:1483915/71‑1 (MQ=255) gcgcCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGtt < 1:726348/71‑1 (MQ=255) cgcCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGtt > 1:945448/1‑70 (MQ=255) gcCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑Ctgt‑‑g < 1:261892/51‑1 (MQ=255) gcCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCa > 1:2894623/1‑71 (MQ=255) ggaattgcctgatgcgctacgcttatcaggcctacgAGTTCAGTG‑‑CTCTACGTAGGCCGGATAAGGc < 1:2998829‑M2/31‑1 (MQ=255) ggaattgcctgatgcgctacgcttatcaggcctacgAGTTCAGTG‑‑CTCTACGTAGGCCGGATAAGGCGtt > 1:4305531‑M2/37‑70 (MQ=255) ggaattgcctgatgcgctacgcttatcaggcctacgAGTTCAGTG‑‑CTCTACGTAGGCCGGATAAGGCGtt > 1:1703141‑M2/37‑70 (MQ=255) ccTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGAt < 1:1373728/60‑1 (MQ=255) gaattgcctgatgcgctacgcttatcaggcctacgAGTTCAGTG‑‑CTCTACGTAGGCCGGATAAGGCGtt < 1:1040741‑M2/34‑1 (MQ=255) gaattgcctgatgcgctacgcttatcaggcctacgAGTTCAGTG‑‑CTCTACGTAGGCCGGATAAGGCGtt < 1:2241225‑M2/34‑1 (MQ=255) gaattgcctgatgcgctacgcttatcaggcctacgAGTTCAGTG‑‑CTCTACGTAGGCCGGATAAGGCGTTCa > 1:2826639‑M2/36‑71 (MQ=255) cTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCAcg > 1:4238028/1‑71 (MQ=255) tGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCAc < 1:2914178/69‑1 (MQ=255) aattgcctgatgcgctacgcttatcaggcctacgAGTTCAGTG‑‑CTCTACGTAGGCCGGATAAGGCGtt < 1:4719136‑M2/34‑1 (MQ=255) ttgcctgatgcgctacgcttatcaggcctacgAGTTCAGTG‑‑CTCTACGTAGGCCGGATAAGGCGTTCAcgc > 1:5025649‑M2/33‑71 (MQ=255) ttgcctgatgcgctacgcttatcaggcctacgAGTTCAGTG‑‑CTCTACGTAGGCCGGATAAGGCGTTCAcgc > 1:1888675‑M2/33‑71 (MQ=255) aTGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCAcgccg < 1:4559033/71‑1 (MQ=255) aTGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCAcgccg > 1:1721923/1‑71 (MQ=255) aTGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCAcgccg > 1:5057695/1‑71 (MQ=255) aTGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCAcgc > 1:491877/1‑69 (MQ=255) aTGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCAcgc > 1:1318144/1‑69 (MQ=255) tgcctgatgcgctacgcttatcaggcctacgAGTTCAGTG‑‑CTCTACGTAGGCCGGATAAGGCGTTCAcgcc > 1:4708630‑M2/32‑71 (MQ=255) tgcctgatgcgctacgcttatcaggcctacgAGTTCAGTG‑‑CTCTACGTAGGCCGGATAAGGCGTTCAcgcc > 1:4258136‑M2/32‑71 (MQ=255) tGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGAt > 1:1858267/1‑55 (MQ=255) gcctgatgcgctacgcttatcaggcctacgAGTTCAGTG‑‑CTCTACGTAGGCCGGATAAGGCGTTCAcgccg > 1:4888826‑M2/31‑71 (MQ=255) cGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCa < 1:4922827/70‑1 (MQ=255) cGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCa < 1:1819582/70‑1 (MQ=255) cGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCAt > 1:2017604/1‑71 (MQ=255) gatgcgctacgcttatcaggcctacgAGTTCAGTG‑‑CTCTACGTAGGCCGGATAAGGCGtt < 1:5394254‑M2/34‑1 (MQ=255) gatgcgctacgcttatcaggcctacgAGTTCAGTG‑‑CTCTACGTAGGCCGGATAAGGCGTTCACGCCGCATc > 1:4182421‑M2/27‑71 (MQ=255) gCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑Ctgt‑‑g > 1:359141/1‑39 (MQ=255) gCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATaa > 1:2659578/1‑51 (MQ=255) gCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATaa > 1:2968987/1‑51 (MQ=255) gCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATa > 1:5563422/1‑50 (MQ=255) gCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATa > 1:4471487/1‑50 (MQ=255) atgcgctacgcttatcaggcctacgAGTTCAGTG‑‑CTCTACGTAGGCCGGATAAGGCGTTCACGCCGCATcc > 1:3107430‑M2/26‑71 (MQ=255) atgcgctacgcttatcaggcctacgAGTTCAGTG‑‑CTCTACGTAGGCCGGATAAGGCGTTCACGCCGCATcc > 1:1673904‑M2/26‑71 (MQ=255) atgcgctacgcttatcaggcctacgAGTTCAGTG‑‑CTCTACGTAGGCCGGATAAGGCGTTCACGCCGCATc > 1:3474405‑M2/26‑70 (MQ=255) cTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGc < 1:4423034/71‑1 (MQ=255) tgttgtGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGc > 1:1607875/1‑70 (MQ=255) gttgtGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCaa > 1:5329858/1‑71 (MQ=255) ttgtGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAt < 1:4786068/71‑1 (MQ=255) acgcttatcaggcctacgAGTTCAGTG‑‑CTCTACGTAGGCCGGATAAGGCGTTCACGCCGCATCCGgcata > 1:2012476‑M2/19‑68 (MQ=255) gtCTTATCATGCCTACGAGTTCGATGCTCTGT‑‑GTAGGCCGGATAAGGTGTTCACGCCGCATCCGGCattg < 1:1293923/70‑4 (MQ=9) gcttatcaggcctacgAGTTCAGTG‑‑CTCTACGTAGGCCGGATAAGGCGTTCACGCCGCATCCGgcata < 1:271830‑M2/52‑3 (MQ=255) ttatcaggcctacgAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATaa > 1:1171725‑M2/15‑40 (MQ=255) ttatcaggcctacgAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATaa > 1:5583384‑M2/15‑40 (MQ=255) ttatcaggcctacgAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATaa > 1:4212871‑M2/15‑40 (MQ=255) aTCAGGCCTACGAGTTCAGTG‑‑CTCTACGTAGGCCGGATaa > 1:3305296/1‑40 (MQ=16) aTCAGGCCTACGAGTTCAGTG‑‑CTCTACGTAGGCCGGATAAGGCGtt < 1:1028943/46‑1 (MQ=12) aTCAGGCCTACGAGTTCAGTG‑‑CTCTACGTAGGCCGGATAAGGCGtt < 1:4962770/46‑1 (MQ=12) tcaggcctacgAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGc < 1:765018‑M2/47‑1 (MQ=255) tcaggcctacgAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGc < 1:2927832‑M2/47‑1 (MQ=255) tcaggcctacgAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTg < 1:1496778‑M2/60‑1 (MQ=255) tcaggcctacgAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTg < 1:3019297‑M2/60‑1 (MQ=255) caggcctacgAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGc > 1:1660041‑M2/11‑71 (MQ=255) caggcctacgAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGc > 1:5031944‑M2/11‑71 (MQ=255) cAGGCCTACGAGTTCAGTG‑‑CTCTACGTAGGCCGGATAAGGCGtt < 1:349833/44‑1 (MQ=12) cAGGCCTACGAGTTCAGTG‑‑CTCTACGTAGGCCGGATAAGGCGTTCa > 1:2941399/1‑46 (MQ=12) aggcctacgAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCAc < 1:2827852‑M2/35‑1 (MQ=255) ggcctacgAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCt < 1:3217045‑M2/63‑1 (MQ=255) gcctacgAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTg < 1:3302510‑M2/64‑1 (MQ=255) gcctacgAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTg < 1:270384‑M2/64‑1 (MQ=255) ctacgAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGc < 1:2745454‑M2/47‑1 (MQ=255) acgAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATaa > 1:4640862‑M2/4‑55 (MQ=255) acgAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATa > 1:4634188‑M2/4‑54 (MQ=255) acgAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACg > 1:1725065‑M2/4‑70 (MQ=255) acgAGTTCAGTG‑‑CTGT‑‑GTACGTCGGATAAGGCGTTCACGCCGCATCCGGCAATaa > 1:1313807‑M2/4‑55 (MQ=255) cgAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAt < 1:3222217‑M2/50‑1 (MQ=255) cgAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAt < 1:4216311‑M2/50‑1 (MQ=255) cgAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATaa > 1:2448353‑M2/3‑54 (MQ=255) cgAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATaa > 1:5196982‑M2/3‑54 (MQ=255) cgAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGc < 1:4029648‑M2/68‑1 (MQ=255) cgAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGc < 1:2146912‑M2/68‑1 (MQ=255) cgAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCa < 1:3559362‑M2/69‑1 (MQ=255) cgAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCCTGCCTGACGc < 1:137436‑M2/68‑1 (MQ=255) gAGTTCAGTG‑‑CTGT‑‑G‑AGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAAt > 1:2675517‑M2/2‑71 (MQ=255) ttCAGTG‑‑CTGT‑‑CTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGc < 1:5680109/54‑1 (MQ=255) cAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATa > 1:3135213/1‑47 (MQ=255) cAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTAct > 1:5107347/1‑71 (MQ=255) cAGTG‑‑CTGT‑‑GTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTAc < 1:2231166/70‑1 (MQ=255) aGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTActc < 1:5446806/71‑1 (MQ=255) aGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTAct < 1:2988778/70‑1 (MQ=255) gTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTActct < 1:5580577/71‑1 (MQ=255) cTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCg > 1:1452985/1‑71 (MQ=255) | AGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG‑‑CTGT‑‑GTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCG > minE/2925701‑2925833 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |