| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 835,011 | G→C | 21.6% | A1157P (GCA→CCA) ‡ | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 835,011 | 0 | G | C | 21.6% | 256.2 / 65.0 | 125 | A1157P (GCA→CCA) ‡ | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (44/54); new base C (16/11); total (60/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
Only 100 of 125 total aligned reads displayed.
GACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAA > minE/834942‑835079 | gACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGc < 1:3507533/71‑1 (MQ=255) gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg < 1:234117/69‑1 (MQ=255) gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg < 1:4852113/69‑1 (MQ=255) gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg < 1:4414455/69‑1 (MQ=255) gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg < 1:1166388/69‑1 (MQ=255) gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg < 1:3777645/69‑1 (MQ=255) gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGa > 1:3396998/1‑71 (MQ=255) gAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGAc > 1:2012149/1‑71 (MQ=255) aaGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGAc < 1:388643/70‑1 (MQ=255) aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGa > 1:1540180/1‑68 (MQ=255) aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa < 1:114199/71‑1 (MQ=255) aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa < 1:5112215/71‑1 (MQ=255) gtattCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCa > 1:4559720/4‑70 (MQ=255) gTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat > 1:277424/1‑71 (MQ=255) gTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat > 1:1337076/1‑71 (MQ=255) gTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat > 1:4671006/1‑71 (MQ=255) gTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat > 1:4921961/1‑71 (MQ=255) ttCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg < 1:3374290/59‑1 (MQ=255) ttCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg < 1:828517/59‑1 (MQ=255) ttCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgat > 1:1140239/1‑71 (MQ=255) cAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGAt > 1:783057/1‑60 (MQ=255) cAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGAt > 1:1074713/1‑60 (MQ=255) cAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTAcc < 1:3832943/71‑1 (MQ=255) gccAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCa > 1:1251324/3‑71 (MQ=255) ccAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCAc > 1:4609626/2‑71 (MQ=255) aaCGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCAcgc > 1:906405/1‑71 (MQ=255) aaCGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCAcgc > 1:828027/1‑71 (MQ=255) aCGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAg < 1:3711934/48‑1 (MQ=255) aCGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTat > 1:1890195/1‑64 (MQ=255) aCGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTat > 1:2962100/1‑64 (MQ=255) aCGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTat > 1:2708417/1‑64 (MQ=255) aCGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCAcgcc > 1:3788152/1‑70 (MQ=255) ggTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCAcgcg > 1:4350576/1‑69 (MQ=255) gTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAg < 1:1291937/71‑1 (MQ=255) tGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAgg < 1:859508/71‑1 (MQ=255) cgctGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGaa < 1:412202/69‑1 (MQ=255) tctGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGc < 1:5557475/39‑1 (MQ=255) ctGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACg > 1:447976/1‑71 (MQ=255) tGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTTCGGCAGGGATGACCATGATGTACCACGCGCAGGAACg > 1:4915988/1‑70 (MQ=255) gACTGCCCGTGCGGTTGTCAGGCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTa > 1:4388736/1‑71 (MQ=255) gACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTa > 1:2540680/1‑71 (MQ=255) aCTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCAt > 1:91440/1‑71 (MQ=255) tGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgatg < 1:1829981/49‑1 (MQ=255) cccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCg < 1:661469/69‑1 (MQ=255) cccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGtt < 1:4116101/71‑1 (MQ=255) cccGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCAttat < 1:3019946/70‑4 (MQ=255) cccGCGCGGTGGTCAACCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCAttat > 1:45183/1‑67 (MQ=255) ccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGt < 1:1621651/64‑1 (MQ=255) ccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGt < 1:3707454/64‑1 (MQ=255) tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa < 1:2461041/38‑1 (MQ=255) tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa < 1:2566865/38‑1 (MQ=255) tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTa > 1:641543/1‑45 (MQ=255) tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTa > 1:293482/1‑45 (MQ=255) tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGtt < 1:2860641/67‑1 (MQ=255) tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGtt < 1:679706/67‑1 (MQ=255) tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcc > 1:2168996/1‑71 (MQ=255) gCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcct > 1:1216563/1‑71 (MQ=255) cGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctg > 1:3045363/1‑71 (MQ=255) cGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctg > 1:4223642/1‑71 (MQ=255) gTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTAc < 1:4478773/42‑1 (MQ=255) ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctgcct < 1:2602551/71‑1 (MQ=255) ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctgcct < 1:1044911/71‑1 (MQ=255) ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctgcct < 1:3365965/71‑1 (MQ=255) gTCAGCCAGCGTGTTCCGGCAGGGATGACCatgatg > 1:1405320/1‑36 (MQ=255) gTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACg > 1:1033878/1‑53 (MQ=255) gTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTgg > 1:4585703/1‑71 (MQ=255) gTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCa < 1:2518405/47‑1 (MQ=16) gTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCa < 1:3277177/47‑1 (MQ=16) cagccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGtt > 1:4710332/1‑71 (MQ=255) cagccaacGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAgg > 1:2456129/1‑47 (MQ=16) cagccaacGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACgc > 1:5210683/1‑51 (MQ=16) agccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTc > 1:832669/1‑71 (MQ=255) agccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTc > 1:839341/1‑71 (MQ=255) agccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTc > 1:2082186/1‑71 (MQ=255) agccaacGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCATTATGAATATTCCTGGTTc > 1:3183756/1‑71 (MQ=255) gccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTa < 1:2997136/57‑1 (MQ=255) gccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTa < 1:4065311/57‑1 (MQ=255) gccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTa < 1:5384197/57‑1 (MQ=255) ccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCgg < 1:4492449/71‑1 (MQ=255) ccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCgg < 1:641250/71‑1 (MQ=255) ccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGCTTCgg < 1:2188890/71‑1 (MQ=255) agcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGa > 1:1111952/1‑70 (MQ=255) agcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGa < 1:5320507/70‑1 (MQ=255) aacGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCATTATGAATATTCCTGGTTCg < 1:1771948/66‑1 (MQ=255) acGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGaa > 1:4853511/2‑43 (MQ=12) cGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCgg < 1:5510869/67‑1 (MQ=255) cGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCgg < 1:3043868/67‑1 (MQ=255) cGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCgg < 1:2805172/67‑1 (MQ=255) gtgtACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCATTATGAATATTCCTGGTTCGGaagta < 1:4552860/71‑4 (MQ=255) gtgtACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCATTATGAATATTCCTGGTTCGGaagt > 1:540723/1‑68 (MQ=255) tgtTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGt < 1:5269924/48‑1 (MQ=255) tgtTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGt < 1:4029514/48‑1 (MQ=255) ttCCGGCAGGGATGACCATGATGTACCACGCGCAg > 1:5210523/1‑35 (MQ=255) ttCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGa < 1:3109862/64‑1 (MQ=255) ttCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTAcc > 1:3714975/1‑71 (MQ=255) ccGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGaaa > 1:5110952/1‑64 (MQ=255) ccGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTAccc > 1:1956105/1‑70 (MQ=255) ccGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTAccc > 1:1409111/1‑70 (MQ=255) cGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCaa < 1:100192/71‑1 (MQ=255) cGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCa < 1:2256105/70‑1 (MQ=255) | GACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAA > minE/834942‑835079 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |