| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 835,037 | G→C | 19.1% | A1165A (GCG→GCC) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 835,037 | 0 | G | C | 19.1% | 262.2 / 49.5 | 115 | A1165A (GCG→GCC) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (35/58); new base C (7/15); total (42/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 124 total aligned reads displayed.
GGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCGGTCA > minE/834969‑835107 | ggTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCAcgcg > 1:4350576/1‑69 (MQ=255) gTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAg < 1:1291937/71‑1 (MQ=255) tGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAgg < 1:859508/71‑1 (MQ=255) cgctGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGaa < 1:412202/69‑1 (MQ=255) ctGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACg > 1:447976/1‑71 (MQ=255) tGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTTCGGCAGGGATGACCATGATGTACCACGCGCAGGAACg > 1:4915988/1‑70 (MQ=255) gACTGCCCGTGCGGTTGTCAGGCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTa > 1:4388736/1‑71 (MQ=255) gACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTa > 1:2540680/1‑71 (MQ=255) cccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCg < 1:661469/69‑1 (MQ=255) cccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGtt < 1:4116101/71‑1 (MQ=255) cccGCGCGGTGGTCAACCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCAttat > 1:45183/1‑67 (MQ=255) ccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGt < 1:1621651/64‑1 (MQ=255) ccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGt < 1:3891855/64‑1 (MQ=255) ccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGt < 1:3707454/64‑1 (MQ=255) gTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTAt < 1:2908133/64‑1 (MQ=255) tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGtt < 1:3040612/67‑1 (MQ=255) tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGtt < 1:2860641/67‑1 (MQ=255) tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGtt < 1:679706/67‑1 (MQ=255) tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcc > 1:2168996/1‑71 (MQ=255) gCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcct > 1:1216563/1‑71 (MQ=255) cGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctg > 1:3045363/1‑71 (MQ=255) ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctgcct < 1:1044911/71‑1 (MQ=255) ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctgcct < 1:3365965/71‑1 (MQ=255) ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctgcct < 1:2602551/71‑1 (MQ=255) gTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACg > 1:1033878/1‑53 (MQ=255) gTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTgg > 1:4585703/1‑71 (MQ=255) gTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCa < 1:2518405/47‑1 (MQ=16) tCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCAt < 1:3224699/55‑1 (MQ=255) cagccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGtt > 1:4710332/1‑71 (MQ=255) cagccaacGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAgg > 1:2456129/1‑47 (MQ=16) cagccaacGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACgc > 1:5210683/1‑51 (MQ=16) agccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTc > 1:2082186/1‑71 (MQ=255) agccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTc > 1:839341/1‑71 (MQ=255) agccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTc > 1:832669/1‑71 (MQ=255) agccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTc > 1:168575/1‑71 (MQ=255) agccaacGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCATTATGAATATTCCTGGTTc > 1:3183756/1‑71 (MQ=255) gccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTa < 1:4065311/57‑1 (MQ=255) gccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTa < 1:5384197/57‑1 (MQ=255) gccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTa < 1:2997136/57‑1 (MQ=255) ccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCgg < 1:641250/71‑1 (MQ=255) ccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCgg < 1:4492449/71‑1 (MQ=255) ccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGCTTCgg < 1:2188890/71‑1 (MQ=255) agcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGa < 1:5320507/70‑1 (MQ=255) agcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGa > 1:1111952/1‑70 (MQ=255) acGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGaa > 1:4853511/2‑43 (MQ=12) cGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCgg < 1:3043868/67‑1 (MQ=255) gtgtACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCATTATGAATATTCCTGGTTCGGaagta < 1:4552860/71‑4 (MQ=255) gtgtACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCATTATGAATATTCCTGGTTCGGaagt > 1:540723/1‑68 (MQ=255) tgtTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGt < 1:5269924/48‑1 (MQ=255) tgtTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGt < 1:4029514/48‑1 (MQ=255) ccGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGaaa > 1:630580/1‑64 (MQ=255) ccGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGaaa > 1:5110952/1‑64 (MQ=255) ccGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTAccc > 1:1956105/1‑70 (MQ=255) ccGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTAccc > 1:1409111/1‑70 (MQ=255) ccGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTAccc > 1:5149989/1‑70 (MQ=255) ccGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTAccc < 1:266157/70‑1 (MQ=255) cGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCaa < 1:100192/71‑1 (MQ=255) cGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCa < 1:2256105/70‑1 (MQ=255) cAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAg < 1:5439251/71‑1 (MQ=255) aGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCaa > 1:3655850/1‑67 (MQ=255) aGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGc < 1:4290125/71‑1 (MQ=255) aGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGc < 1:2919289/71‑1 (MQ=255) aGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGc > 1:2105595/1‑71 (MQ=255) aGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGc < 1:3324322/71‑1 (MQ=255) aGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGc > 1:5220430/1‑71 (MQ=255) aGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGc < 1:711814/71‑1 (MQ=255) gggATGACCATGATGTACCACGCGCAGTAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCCACAGCg < 1:740230/71‑1 (MQ=255) gggATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCg < 1:1873344/71‑1 (MQ=255) gggATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCg > 1:32498/1‑71 (MQ=255) aTGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCgtgg < 1:4157222/71‑1 (MQ=255) aTGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCgtgg < 1:1870127/71‑1 (MQ=255) tGACCATGATGTATCACGCCCAGGAACGCATTATGAATATTCCTGGTTCGGaagt < 1:5571842/55‑3 (MQ=255) tGACCATGATGTATCACGCCCAGGAACGCATTATGAATATTCCTGGTTCGGaagt < 1:5560455/55‑3 (MQ=255) gACCATGATGTATCACGCCCAGGAACGCATTATGAATATTCCTGGTTCgg < 1:3391191/50‑1 (MQ=255) gACCATGATGTACCACGCGCAGGAACGTATCGTTAAcc < 1:4424211/38‑1 (MQ=255) gACCATGATGTACCACGCGCAGGAACGTATCGTTAAcc < 1:4516214/38‑1 (MQ=255) gACCATGATGTACCACGCGCAGGAACGTATCGTTAAcc < 1:1715527/38‑1 (MQ=255) aCCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTAc > 1:3980459/1‑56 (MQ=255) ccATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTggtggt < 1:3594538/71‑1 (MQ=255) tgatgTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGtggtgg > 1:3078849/1‑67 (MQ=255) tgatgTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATc > 1:4629983/1‑71 (MQ=255) gatgTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTggtggt < 1:5001685/67‑1 (MQ=255) atgTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATcc < 1:4719251/70‑1 (MQ=255) atgTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATcc < 1:5501726/70‑1 (MQ=255) atgTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCa < 1:2565994/71‑1 (MQ=255) tgTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCAt < 1:1066530/71‑1 (MQ=255) tgTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCAt < 1:755176/71‑1 (MQ=255) tgTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCAt < 1:2338144/71‑1 (MQ=255) tACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATaa > 1:4669266/1‑71 (MQ=255) ccACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACt < 1:1293248/71‑1 (MQ=255) cACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGaaa > 1:2583663/1‑40 (MQ=255) cACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCa < 1:4578529/65‑1 (MQ=255) aCGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCg > 1:316830/1‑71 (MQ=255) cgcgCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCg < 1:1304411/51‑1 (MQ=255) cgcgCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCg < 1:3891796/51‑1 (MQ=255) cgcgCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCg < 1:347520/51‑1 (MQ=255) cgcgCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCgg > 1:3817086/1‑71 (MQ=255) cgcgCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCgg > 1:3692524/1‑71 (MQ=255) cgcgCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCgg > 1:4904930/1‑71 (MQ=255) gcAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCGGTCa > 1:179856/1‑71 (MQ=255) | GGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCGGTCA > minE/834969‑835107 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |