| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 835,148 | T→C | 22.6% | Y1202Y (TAT→TAC) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 835,148 | 0 | T | C | 22.6% | 207.4 / 60.6 | 115 | Y1202Y (TAT→TAC) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (38/51); new base C (16/10); total (54/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 | |||||||||||
Only 100 of 116 total aligned reads displayed.
CAGCGTGGTGGTATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGT > minE/835080‑835216 | cAGCGTGGTGGTATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGc > 1:1272962/1‑71 (MQ=255) gtggtgGTATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCAt < 1:3182946/71‑1 (MQ=255) gtggtgGTATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCAt < 1:3274302/71‑1 (MQ=255) gtggtgGTATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCAt < 1:3347137/71‑1 (MQ=255) ggtggtATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCt > 1:1600360/1‑71 (MQ=255) gtATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGc > 1:5168995/1‑70 (MQ=255) gtATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCa > 1:325779/1‑71 (MQ=255) gtATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCa < 1:3411705/71‑1 (MQ=255) gtATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCa > 1:2730619/1‑71 (MQ=255) cATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACg < 1:33495/70‑1 (MQ=255) aTAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGc > 1:1804288/1‑71 (MQ=255) aCTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCa < 1:2132096/62‑1 (MQ=255) aCTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCttt < 1:5049240/71‑1 (MQ=255) aCTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCtt < 1:4279327/70‑1 (MQ=255) aCTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCt > 1:1977432/1‑69 (MQ=255) aCTCGGTCACCCGTATCACGCAGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCttt < 1:1174669/71‑1 (MQ=255) tCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGCCTATGCCCATCTGGCATACGGCttt < 1:1404137/69‑1 (MQ=255) cGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAac > 1:5690437/1‑71 (MQ=255) gTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCa < 1:3962265/57‑1 (MQ=255) aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAActacta > 1:4035309/1‑71 (MQ=255) ccGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGccc < 1:3886358/44‑1 (MQ=255) ccGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGccc < 1:3776229/44‑1 (MQ=255) ccGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATg > 1:636062/1‑71 (MQ=255) ccGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATg > 1:2121472/1‑71 (MQ=255) cagTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAActacta > 1:629274/3‑69 (MQ=255) gTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGc < 1:3519468/71‑1 (MQ=255) gTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGc < 1:4671923/71‑1 (MQ=255) aTCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCAt > 1:2578909/1‑42 (MQ=255) tCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCGATCTGGCATACGGCTTTAACTACTATGGCAcc > 1:2397428/1‑71 (MQ=255) tCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTgg < 1:4887088/45‑1 (MQ=255) tCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTgg < 1:874635/45‑1 (MQ=255) tCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCAcc > 1:5184292/1‑71 (MQ=255) tCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCAcc > 1:916686/1‑71 (MQ=255) tgcccGAAACCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGt > 1:1140659/4‑71 (MQ=255) tgcccGAAACCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGt > 1:1028244/4‑71 (MQ=255) aCGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCg < 1:4760013/70‑1 (MQ=255) aCGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCg < 1:3495520/70‑1 (MQ=255) aCGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCg > 1:5120562/1‑70 (MQ=255) aCGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCg < 1:659377/70‑1 (MQ=255) aCGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGt < 1:263755/71‑1 (MQ=255) gcccGAAACCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGtc > 1:1715357/3‑70 (MQ=255) gCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCttt < 1:2557802/52‑1 (MQ=255) gCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAg < 1:829241/71‑1 (MQ=255) ccGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGt < 1:2645374/68‑1 (MQ=255) cGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGt > 1:188003/1‑71 (MQ=255) gAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCttt < 1:4849503/49‑1 (MQ=255) gAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAg > 1:1201454/1‑68 (MQ=255) gAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGtt > 1:936588/1‑71 (MQ=255) gAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGtt < 1:2364718/71‑1 (MQ=255) gAAACCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCggat > 1:1957960/1‑69 (MQ=255) gAAACCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCggat > 1:1801658/1‑69 (MQ=255) aaaCCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTc > 1:1058404/1‑71 (MQ=255) aaCCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTaa > 1:462480/1‑49 (MQ=255) aaCCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCt < 1:5628827/71‑1 (MQ=255) aaCCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATcg < 1:2752210/71‑2 (MQ=255) aaCCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATc > 1:2485470/1‑70 (MQ=255) aCCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCttt < 1:4869451/46‑1 (MQ=255) aCCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATcga < 1:1155060/71‑3 (MQ=255) ccaaCGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATCGaa < 1:2363058/68‑1 (MQ=255) ccGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTaa < 1:3133452/71‑1 (MQ=255) gACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCttt < 1:3878354/43‑1 (MQ=255) aCGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATCGAACcg > 1:194914/1‑71 (MQ=255) gCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACcgc > 1:1627846/1‑70 (MQ=255) atatGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATCGAACCGCGAt < 1:2074863/71‑1 (MQ=255) atatGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGAt < 1:1623867/71‑1 (MQ=255) tatGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCGCCGtcg < 1:1800593/56‑3 (MQ=255) tatGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGtcg > 1:4731269/1‑54 (MQ=255) tatGATCGGCGGCTATGCCCATCTGGCATACGGCttt < 1:4150475/37‑1 (MQ=255) tatGATCGGCGGCTATGCCCATCTGGCATACGGCttt < 1:3473633/37‑1 (MQ=255) tatGATCGGCGGCTATGCCCATCTGGCATACGGCttt < 1:737849/37‑1 (MQ=255) tatGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCt < 1:2636473/61‑1 (MQ=255) tatGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATg < 1:387810/71‑1 (MQ=255) tatGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATg < 1:5284471/71‑1 (MQ=255) atGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCt < 1:3015777/60‑1 (MQ=255) tGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATCGAACCGCGAt > 1:480016/1‑68 (MQ=255) tGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATCGAACCGCGATg < 1:1835323/69‑1 (MQ=255) tGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATCGAACCGCGATg < 1:1093244/69‑1 (MQ=255) tGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATCGAACCGCGATGa > 1:3248356/1‑70 (MQ=255) tGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATCGAACCGCGATGa < 1:3098752/70‑1 (MQ=255) tGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATCGAACCGCGATGAg > 1:4471661/1‑71 (MQ=255) tGATCGGCGGCTATGCCCATCTGGCATACGGCTTTaa > 1:5210063/1‑37 (MQ=255) tGATCGGCGGCTATGCCCATCTGGCATACGGCTTTaa > 1:4231638/1‑37 (MQ=255) tGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAg < 1:5188796/71‑1 (MQ=255) gATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATCGAACCGCGATGAGt > 1:805842/1‑71 (MQ=255) gATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCg > 1:2545977/1‑50 (MQ=255) gATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCg > 1:2255828/1‑50 (MQ=255) gATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCg > 1:3029433/1‑50 (MQ=255) gATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCg > 1:3139929/1‑50 (MQ=255) gATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGt > 1:2967366/1‑71 (MQ=255) ttggcggtTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATCGAACCGCGATGAGttc < 1:4466787/69‑2 (MQ=255) ttggcggtTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATCGAACCGCGATGAGttc < 1:3090538/69‑2 (MQ=255) tggcggtTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATCGAACCGCGATGAGttca > 1:4320174/2‑69 (MQ=255) gcggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGt < 1:2652078/46‑1 (MQ=255) cggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg < 1:3619986/71‑1 (MQ=255) cggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg < 1:809850/71‑1 (MQ=255) cTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTa > 1:3173179/1‑70 (MQ=255) cTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACAGCGATGAGttt > 1:4370012/1‑64 (MQ=255) aTGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTATGTTCTAACcgcg < 1:3713206/54‑1 (MQ=255) aTGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACcgcg < 1:324015/54‑1 (MQ=255) tGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttg < 1:4504329/62‑1 (MQ=255) | CAGCGTGGTGGTATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGT > minE/835080‑835216 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |