| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 835,291 | T→C | 27.3% | I3I (ATT→ATC) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 835,291 | 0 | T | C | 27.3% | 209.3 / 92.7 | 139 | I3I (ATT→ATC) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base T (49/52); new base C (23/15); total (72/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 140 total aligned reads displayed.
ATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGT > minE/835224‑835360 | tGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGtt < 1:4242196/71‑1 (MQ=255) tGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGt > 1:5310696/1‑70 (MQ=255) gaagaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGtt > 1:2637404/1‑70 (MQ=255) aagaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTc < 1:4788100/70‑1 (MQ=255) agaaCGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTcac < 1:2320362/71‑1 (MQ=255) agaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTca > 1:3760167/1‑70 (MQ=255) gaaCGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTcaca < 1:3837257/71‑1 (MQ=255) gaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcaca < 1:5561609/71‑1 (MQ=255) gaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcaca < 1:1974923/71‑1 (MQ=255) cATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGt > 1:4031414/1‑71 (MQ=255) gACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCgg < 1:4125809/70‑1 (MQ=255) cTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCg > 1:4727742/1‑56 (MQ=255) ggTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACaa < 1:1517586/61‑1 (MQ=255) ggTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACaa < 1:5350169/61‑1 (MQ=255) gTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATg < 1:5165659/69‑1 (MQ=255) gATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCt > 1:3769060/1‑70 (MQ=255) gATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTg < 1:236991/71‑1 (MQ=255) ggCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcaca > 1:2531585/1‑52 (MQ=255) gCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtc < 1:1585040/71‑1 (MQ=255) gAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATTGTGCTGAAtctc < 1:5349937/71‑1 (MQ=255) aaGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCg < 1:3902747/71‑1 (MQ=255) ggCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt < 1:4381332/71‑1 (MQ=255) gCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcac < 1:5511454/44‑1 (MQ=255) gCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt < 1:4920049/70‑1 (MQ=255) cAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATaa < 1:3129607/71‑1 (MQ=255) aaTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGtt > 1:3066947/1‑39 (MQ=255) aaTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGtt > 1:3968704/1‑39 (MQ=255) aaTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt > 1:178519/1‑68 (MQ=255) aaTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:4613436/1‑71 (MQ=255) aTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCgg < 1:771026/48‑1 (MQ=255) aCCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGtt > 1:1177935/1‑35 (MQ=255) aCCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCa > 1:233830/1‑71 (MQ=255) tcAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTAt > 1:3005167/2‑71 (MQ=255) tcAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTAt > 1:2954352/2‑71 (MQ=255) ccAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCa < 1:3122629/70‑1 (MQ=255) cAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATgt > 1:484887/1‑67 (MQ=255) cAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATc > 1:2118422/1‑71 (MQ=255) cAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATc > 1:556670/1‑71 (MQ=255) aaGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGt > 1:3302333/2‑40 (MQ=12) aGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCg > 1:1446184/1‑71 (MQ=255) aGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACaaat > 1:4363597/1‑63 (MQ=255) aCAGGAGAGCG‑TAAAATGAGAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTg > 1:2227744/1‑71 (MQ=255) aCAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATg > 1:383081/1‑42 (MQ=255) aCAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATg > 1:916219/1‑42 (MQ=255) gagaGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCt < 1:2098594/43‑1 (MQ=255) gagaGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCcac < 1:3211809/71‑1 (MQ=255) agagCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtct > 1:3287573/1‑48 (MQ=255) agCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACAcc < 1:104920/71‑1 (MQ=255) cG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGa > 1:3072600/1‑40 (MQ=255) cG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGa > 1:2775739/1‑40 (MQ=255) aaaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGt < 1:2601292/69‑1 (MQ=255) aaaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGt < 1:892720/69‑1 (MQ=255) tAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGtt < 1:829840/71‑1 (MQ=255) aaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGc > 1:1924013/2‑61 (MQ=255) aaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGtt < 1:3431545/70‑1 (MQ=255) aaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGtt < 1:4462691/70‑1 (MQ=255) aaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGATTAACCTCGAGAAATGTATCGGCTGCCATACCTGtt < 1:515880/70‑1 (MQ=255) aaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTAt > 1:3815755/1‑53 (MQ=255) aaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTc > 1:2363345/1‑71 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGaa > 1:926853/1‑37 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGaa > 1:3621258/1‑37 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGaa > 1:3377056/1‑37 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGaa > 1:2832921/1‑37 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGa < 1:5611580/36‑1 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGa > 1:4103550/1‑43 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGa > 1:485090/1‑43 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATa < 1:2027004/45‑1 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATa < 1:5139248/45‑1 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATa < 1:4720835/45‑1 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGt < 1:4634673/48‑1 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGtt > 1:1677326/1‑69 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTc < 1:4398764/70‑1 (MQ=255) aaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTaac > 1:1661258/1‑37 (MQ=38) aaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTaac > 1:4297083/1‑37 (MQ=38) aaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTaac > 1:1654626/1‑37 (MQ=38) aaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTcg > 1:2178191/1‑70 (MQ=255) aaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATgt < 1:1707439/49‑2 (MQ=255) aaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATgt < 1:2977072/49‑2 (MQ=255) aTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGt > 1:2942982/1‑71 (MQ=255) tGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTc < 1:2007373/67‑1 (MQ=255) tGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATg < 1:1345615/46‑1 (MQ=255) aaaaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTTCCACACCTGTTCAGTTAc > 1:1753273/1‑71 (MQ=255) aaaaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTa < 1:2111745/70‑1 (MQ=255) aaaaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTAc > 1:2988737/1‑71 (MQ=255) aaaaTCCGTTCACAAGTCGGCATGGTGCTTAACCTCGaca < 1:5583323/40‑3 (MQ=255) aaaaTCCGTTCACAAGTCGGCATGGTGCTTAACCTCGaca < 1:5576613/40‑3 (MQ=255) aaaTCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGAcc > 1:3033300/1‑71 (MQ=255) aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:1649166/1‑40 (MQ=255) aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:844935/1‑40 (MQ=255) aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:3674212/1‑40 (MQ=255) aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:1941724/1‑40 (MQ=255) aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:4641487/1‑40 (MQ=255) aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTc < 1:643041/63‑1 (MQ=255) aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTAcc < 1:5463800/70‑1 (MQ=255) aaTCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCt < 1:3422085/71‑1 (MQ=255) aTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCAt > 1:1975393/1‑44 (MQ=255) aTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCAt > 1:2679041/1‑44 (MQ=255) aTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCAt > 1:4538003/1‑44 (MQ=255) aTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAg > 1:5531578/1‑64 (MQ=255) ttCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGt < 1:1202460/71‑1 (MQ=255) | ATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGT > minE/835224‑835360 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |