| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 835,315 | G→T | 39.9% | L11L (CTG→CTT) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 835,315 | 0 | G | T | 39.9% | 146.5 / 125.6 | 135 | L11L (CTG→CTT) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base G (41/40); new base T (29/25); total (70/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 136 total aligned reads displayed.
GATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGA > minE/835245‑835383 | gATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTg < 1:236991/71‑1 (MQ=255) aTGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGa < 1:2242913/71‑1 (MQ=255) gCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtc < 1:1585040/71‑1 (MQ=255) gAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATTGTGCTGAAtctc < 1:5349937/71‑1 (MQ=255) aaGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCg < 1:3902747/71‑1 (MQ=255) ggCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt < 1:4381332/71‑1 (MQ=255) aaTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:4613436/1‑71 (MQ=255) aaTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:4002944/1‑71 (MQ=255) aaTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGGGCTGAATCTCGATaa < 1:5613257/70‑1 (MQ=255) aTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:1670486/1‑70 (MQ=255) tcAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTAt > 1:3005167/2‑71 (MQ=255) tcAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTAt > 1:2954352/2‑71 (MQ=255) ccAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCa < 1:3122629/70‑1 (MQ=255) cAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATc > 1:556670/1‑71 (MQ=255) cAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATc > 1:2118422/1‑71 (MQ=255) aGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCg > 1:1446184/1‑71 (MQ=255) aGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACaaat > 1:2580379/1‑63 (MQ=255) aGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACaaat > 1:2366678/1‑63 (MQ=255) aGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACaaat > 1:2155698/1‑63 (MQ=255) gTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt < 1:4453539/59‑1 (MQ=255) aCAGGAGAGCG‑TAAAATGAGAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTg > 1:2227744/1‑71 (MQ=255) aCAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTg > 1:1495309/1‑71 (MQ=255) gagaGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCcac < 1:3211809/71‑1 (MQ=255) agagCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtct > 1:3287573/1‑48 (MQ=255) agCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACAcc < 1:104920/71‑1 (MQ=255) cG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGa > 1:3072600/1‑40 (MQ=255) cG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGa > 1:2775739/1‑40 (MQ=255) aaaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGt < 1:892720/69‑1 (MQ=255) aaaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGt < 1:2601292/69‑1 (MQ=255) tAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGtt < 1:829840/71‑1 (MQ=255) aaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGc > 1:458403/2‑61 (MQ=255) aaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGc > 1:1924013/2‑61 (MQ=255) aaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGc > 1:124944/2‑61 (MQ=255) aaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGtt < 1:4462691/70‑1 (MQ=255) aaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGtt < 1:3431545/70‑1 (MQ=255) aaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTAt > 1:3815755/1‑53 (MQ=255) aaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTc > 1:2363345/1‑71 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGaa > 1:3377056/1‑37 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGaa > 1:3621258/1‑37 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGa < 1:5326309/36‑1 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGa > 1:4103550/1‑43 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGa > 1:485090/1‑43 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATa < 1:2027004/45‑1 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATa < 1:5139248/45‑1 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATa < 1:4720835/45‑1 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGt < 1:4634673/48‑1 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGt < 1:3425474/48‑1 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTc < 1:4398764/70‑1 (MQ=255) aaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTaac > 1:4297083/1‑37 (MQ=38) aaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTaac > 1:1661258/1‑37 (MQ=38) aaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTaac > 1:1654626/1‑37 (MQ=38) aaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTcg > 1:2178191/1‑70 (MQ=255) aaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATgt < 1:2977072/49‑2 (MQ=255) aaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATgt < 1:1707439/49‑2 (MQ=255) aTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGt > 1:2942982/1‑71 (MQ=255) tGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTc < 1:2007373/67‑1 (MQ=255) tGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATg < 1:3807655/46‑1 (MQ=255) tGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATg < 1:1345615/46‑1 (MQ=255) aaaaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTTCCACACCTGTTCAGTTAc > 1:1753273/1‑71 (MQ=255) aaaaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTAc > 1:2988737/1‑71 (MQ=255) aaaaTCCGTTCACAAGTCGGCATGGTGCTTAACCTCGaca < 1:5576613/40‑3 (MQ=255) aaaaTCCGTTCACAAGTCGGCATGGTGCTTAACCTCGaca < 1:5583323/40‑3 (MQ=255) aaaTCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGAcc > 1:3033300/1‑71 (MQ=255) aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:844935/1‑40 (MQ=255) aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:1941724/1‑40 (MQ=255) aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:4641487/1‑40 (MQ=255) aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:31596/1‑40 (MQ=255) aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTAcc < 1:5463800/70‑1 (MQ=255) aTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCAt > 1:4538003/1‑44 (MQ=255) aTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCAt > 1:2679041/1‑44 (MQ=255) aTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAg > 1:5531578/1‑64 (MQ=255) ttCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGt < 1:1202460/71‑1 (MQ=255) tccGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGtg < 1:2521365/63‑2 (MQ=255) ccGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTg > 1:4929890/2‑69 (MQ=255) gTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTaaa < 1:39445/71‑1 (MQ=255) gTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTaa < 1:3824596/70‑1 (MQ=255) gTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTaa < 1:2735648/70‑1 (MQ=255) ttCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGtt < 1:1244187/56‑1 (MQ=255) ttCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTaaa < 1:2080557/70‑1 (MQ=255) tCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGc < 1:2813408/45‑1 (MQ=255) cacaAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCca > 1:5225096/1‑46 (MQ=255) cacaAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAAc > 1:2548851/1‑71 (MQ=255) acaAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACg > 1:3183088/1‑71 (MQ=255) caAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGtt < 1:807893/52‑1 (MQ=255) aaGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTaaaa > 1:5685662/1‑66 (MQ=255) aGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCt > 1:503056/1‑71 (MQ=255) tCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTgg < 1:5621635/71‑1 (MQ=255) ggCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGAc < 1:4918992/71‑1 (MQ=255) aTGGTGCTGAATCTCTATAAGTGCATCGGCTGCCACAcc < 1:1000773/39‑1 (MQ=255) tGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGtt < 1:1798066/42‑1 (MQ=255) tGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTc < 1:2512733/43‑1 (MQ=255) tGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTc < 1:836334/43‑1 (MQ=255) tGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTc < 1:4865397/43‑1 (MQ=255) gTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTg > 1:4608998/1‑62 (MQ=255) gTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCg < 1:696788/71‑1 (MQ=255) gCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTa > 1:921225/1‑50 (MQ=255) gCTGAATCTCGATAAGTGCATCGGCTTCCACACCTGTTCAGTTACCTGTaa < 1:757831/51‑1 (MQ=255) gCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGt > 1:4773351/1‑70 (MQ=255) cTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGt > 1:4223772/1‑69 (MQ=255) cTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTg < 1:4804319/70‑1 (MQ=255) | GATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGA > minE/835245‑835383 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |