| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 835,414 | G→C | 19.1% | V44V (GTG→GTC) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 835,414 | 0 | G | C | 19.1% | 97.6 / 17.4 | 47 | V44V (GTG→GTC) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base G (17/21); new base C (6/3); total (23/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
TTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGAT > minE/835348‑835482 | ttCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGaaa > 1:1289810/1‑71 (MQ=255)ttCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGaaa > 1:2403902/1‑71 (MQ=255)ttCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGa < 1:1053184/69‑1 (MQ=255) gtgaCCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGAAACCaa < 1:3125305/68‑1 (MQ=255) gtgaCCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGAAACCaa < 1:2572197/68‑1 (MQ=255) ttACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAg > 1:3842573/1‑71 (MQ=255) ttACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAg > 1:5082360/1‑71 (MQ=255) ttACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAg > 1:1586002/1‑71 (MQ=255) gaCCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGAAACCaaac > 1:3144337/2‑69 (MQ=255) ccTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCa < 1:314561/66‑1 (MQ=255) tGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCggg > 1:5021954/1‑71 (MQ=255) gTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGAAACCAAACCggg > 1:3579405/1‑70 (MQ=255) gTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGa > 1:2549367/1‑58 (MQ=255) gTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGa > 1:1070581/1‑58 (MQ=255) gTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCggg > 1:65983/1‑70 (MQ=255) tAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGAAACCAAACCggg < 1:5453935/69‑1 (MQ=255) tAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGa < 1:2172006/57‑1 (MQ=255) tAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGa < 1:2614785/57‑1 (MQ=255) tAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGc < 1:5391119/70‑1 (MQ=255) aaaaaCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGc > 1:763680/1‑69 (MQ=255) aaaaCGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGAAACCAAACCGGGcatt > 1:3831433/1‑68 (MQ=255) aaaaCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCaa > 1:4597067/1‑61 (MQ=255) aaaaCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCaa > 1:2311803/1‑61 (MQ=255) aaaaCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAg < 1:5291826/71‑1 (MQ=255) aaaCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTg < 1:2925466/52‑1 (MQ=255) aaaCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTg < 1:3860438/52‑1 (MQ=255) aaaCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTg < 1:3225637/52‑1 (MQ=255) aaaCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTg < 1:3669423/52‑1 (MQ=255) aaaCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCGGGGCCa < 1:7934/69‑1 (MQ=255) aaaCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCa < 1:1014431/69‑1 (MQ=255) aCGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGaaa > 1:1029114/1‑54 (MQ=255) gTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCtt > 1:1468629/1‑71 (MQ=255) tGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTccc > 1:2553376/1‑71 (MQ=255) gACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTccc < 1:116334/69‑1 (MQ=255) gACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTccc < 1:5587888/69‑1 (MQ=255) gCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCgactgac < 1:4536268/71‑1 (MQ=255) gCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCgactgac < 1:5170359/71‑1 (MQ=255) gCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCgactgac < 1:2235549/71‑1 (MQ=255) ccGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCgactgact < 1:2013259/71‑1 (MQ=255) gTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTgg < 1:1987430/71‑1 (MQ=255) aaGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGa < 1:591039/70‑1 (MQ=255) aGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGaaa < 1:4420474/71‑1 (MQ=255) cGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCtt < 1:4216180/51‑1 (MQ=255) tGGAATATGCGTGGTTCAACAACGTGGAAACCAAGc < 1:3736977/36‑1 (MQ=255) tGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGcc < 1:2714899/42‑1 (MQ=255) ggAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAg > 1:719327/1‑70 (MQ=255) gAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTccc > 1:2942501/1‑50 (MQ=255) gAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTcc > 1:3956612/1‑49 (MQ=255) tGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGaaaaa < 1:165773/70‑1 (MQ=255) gCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATa > 1:2757157/1‑71 (MQ=255) gTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGAt > 1:810920/1‑71 (MQ=255) | TTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGAT > minE/835348‑835482 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |