| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 835,891 | G→A | 13.7% | K203K (AAG→AAA) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 835,891 | 0 | G | A | 13.7% | 238.8 / 22.9 | 95 | K203K (AAG→AAA) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base G (27/55); new base A (4/9); total (31/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGG > minE/835821‑835959 | tGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACATGCCCGAGCGGTGCGATTTACAAg > 1:4295982/1‑71 (MQ=255) ccGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCg > 1:4131440/1‑71 (MQ=255) ccGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGT‑GCGACCTGCCCGAGCGGTGCGATTTACAAGCGt > 1:4347390/1‑71 (MQ=255) gcCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTga < 1:1424948/70‑1 (MQ=255) gcCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACGCgaa < 1:4008683/71‑1 (MQ=255) tgtgCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACGCgaag < 1:5114094/69‑1 (MQ=255) gtgCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaa > 1:3075944/1‑67 (MQ=255) tgCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa < 1:5674081/69‑1 (MQ=255) tgCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACGCgaagaa < 1:1690358/69‑1 (MQ=255) tgCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACGCgaagaa < 1:5420271/69‑1 (MQ=255) cGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCg < 1:4920128/60‑1 (MQ=255) cGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTg > 1:1109417/1‑62 (MQ=255) gAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAagaaga > 1:5406577/1‑68 (MQ=255) aaCACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATgg > 1:2365420/1‑70 (MQ=255) aaCACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAagaagacg < 1:809713/69‑3 (MQ=255) cTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa < 1:4544790/61‑1 (MQ=255) cTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCg < 1:3433784/71‑1 (MQ=255) tGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATTGCATCGTCCt > 1:854048/1‑70 (MQ=255) tGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATc > 1:1163641/1‑65 (MQ=255) tGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCt > 1:5067144/1‑70 (MQ=255) aa‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaag > 1:3137770/1‑52 (MQ=255) aa‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGGGAAGAAGATGGCATCGTCCTGAt < 1:4859008/71‑1 (MQ=255) cccGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCg < 1:4446315/71‑1 (MQ=255) ccGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa < 1:998695/51‑1 (MQ=255) cGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGaagaag < 1:3814880/51‑1 (MQ=255) cGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGAc < 1:2858466/71‑1 (MQ=255) ggCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGaagaag < 1:2387615/50‑1 (MQ=255) ggCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGAcc < 1:4683953/71‑1 (MQ=255) cATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGaagaag < 1:4593058/48‑1 (MQ=255) tgtgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa < 1:2313650/45‑1 (MQ=255) tgcgtgGCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGa > 1:2592635/4‑71 (MQ=255) gtgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa < 1:1287185/44‑1 (MQ=255) gtgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa < 1:1390108/44‑1 (MQ=255) gtgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa < 1:5629327/44‑1 (MQ=255) gtgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa < 1:543383/44‑1 (MQ=255) gtgtgGCGACCTGCCCGAGCGGGGCGATTTACAAGCGTgaagaa < 1:1740031/44‑1 (MQ=255) gcgtgGCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGa > 1:4385442/3‑70 (MQ=255) tgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGAt > 1:456002/1‑70 (MQ=255) tgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGAt > 1:4396349/1‑70 (MQ=255) tgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGAt > 1:1775170/1‑70 (MQ=255) tgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGAt > 1:534635/1‑70 (MQ=255) tgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGAt > 1:2016248/1‑70 (MQ=255) gCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaaa > 1:5052181/1‑69 (MQ=255) cTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTcc > 1:2232410/1‑47 (MQ=255) cTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGtg < 1:4317549/71‑1 (MQ=255) gCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTg > 1:3817871/1‑47 (MQ=255) cccGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGtggc < 1:512664/70‑1 (MQ=255) cccAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGtggc < 1:2476477/70‑1 (MQ=255) cGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGtggctgg > 1:5396429/1‑71 (MQ=255) aGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCg < 1:4305586/71‑1 (MQ=255) cGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATg < 1:4212846/57‑1 (MQ=255) cGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGtt < 1:3102966/71‑2 (MQ=255) ggTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAt < 1:2869767/71‑1 (MQ=255) gTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGAcc < 1:4924439/45‑1 (MQ=255) gTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGAcc < 1:3894254/45‑1 (MQ=255) gTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGAcc < 1:3645624/45‑1 (MQ=255) tGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATa < 1:4264622/50‑1 (MQ=255) tGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGtggc < 1:2644161/61‑1 (MQ=255) tGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTa < 1:1157081/68‑1 (MQ=255) tGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgt < 1:3130205/71‑1 (MQ=255) tGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATAGACCAGGATa < 1:4733414/50‑1 (MQ=255) gCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgtg > 1:3749414/1‑71 (MQ=255) gCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgtg > 1:3141955/1‑71 (MQ=255) cGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgtg > 1:5115664/1‑70 (MQ=255) aTTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCAt < 1:3329174/71‑1 (MQ=255) aTTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCAt < 1:2886675/71‑1 (MQ=255) aTTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCAt < 1:4883487/71‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATc < 1:936548/35‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATc < 1:821844/35‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATc < 1:1611923/35‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATc < 1:2013520/35‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATa < 1:3165576/45‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATa < 1:262897/45‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:870498/71‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:2778101/71‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:564971/71‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:371163/71‑1 (MQ=255) tctACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGtt < 1:4137480/61‑2 (MQ=255) ctACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCAt > 1:4756963/2‑69 (MQ=255) tACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:4344729/69‑1 (MQ=255) tACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:2259651/69‑1 (MQ=255) tACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:4979942/69‑1 (MQ=255) tACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:2745206/69‑1 (MQ=255) tACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCa > 1:4708042/1‑70 (MQ=255) tACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCa < 1:1514189/70‑1 (MQ=255) tACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCAc > 1:5361167/1‑71 (MQ=255) tACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCAc > 1:5483122/1‑71 (MQ=255) tACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCAtaag > 1:3331763/1‑68 (MQ=255) cAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg < 1:2055133/71‑1 (MQ=255) cAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg < 1:1800628/71‑1 (MQ=255) cAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg < 1:1970781/71‑1 (MQ=255) cAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg < 1:5134126/71‑1 (MQ=255) cAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCAtaag < 1:1404041/69‑4 (MQ=255) aaGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTgg < 1:1942584/71‑1 (MQ=255) aGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGtg > 1:1531544/1‑48 (MQ=255) aGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGtg > 1:2214513/1‑48 (MQ=255) gCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaa > 1:755488/1‑39 (MQ=255) gCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaa > 1:903774/1‑39 (MQ=255) gCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtg > 1:128815/1‑58 (MQ=255) | TGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGG > minE/835821‑835959 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |