| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 835,946 | A→T | 28.7% | M222L (ATG→TTG) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 835,946 | 0 | A | T | 28.7% | 175.3 / 85.5 | 108 | M222L (ATG→TTG) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base A (32/45); new base T (16/15); total (48/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 109 total aligned reads displayed.
GGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGC > minE/835877‑836014 | ggTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAt < 1:2869767/71‑1 (MQ=255) tGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTa < 1:1157081/68‑1 (MQ=255) tGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgt < 1:3130205/71‑1 (MQ=255) aTTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCAt < 1:4883487/71‑1 (MQ=255) aTTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCAt < 1:3329174/71‑1 (MQ=255) aTTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCAt < 1:2886675/71‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:870498/71‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:564971/71‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:371163/71‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:2778101/71‑1 (MQ=255) ctACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCAt > 1:4756963/2‑69 (MQ=255) tACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:2745206/69‑1 (MQ=255) tACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:4344729/69‑1 (MQ=255) tACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:2259651/69‑1 (MQ=255) tACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:4979942/69‑1 (MQ=255) tACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCa < 1:1514189/70‑1 (MQ=255) tACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCa > 1:4708042/1‑70 (MQ=255) tACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCAc > 1:5361167/1‑71 (MQ=255) tACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCAc > 1:5483122/1‑71 (MQ=255) tACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCAtaag > 1:3331763/1‑68 (MQ=255) cAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg < 1:1970781/71‑1 (MQ=255) cAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg < 1:2055133/71‑1 (MQ=255) cAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg < 1:5134126/71‑1 (MQ=255) cAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg < 1:1800628/71‑1 (MQ=255) cAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCAtaag < 1:1404041/69‑4 (MQ=255) aaGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTgg < 1:1942584/71‑1 (MQ=255) gCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtg > 1:128815/1‑58 (MQ=255) gaagaTGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCa > 1:1324929/1‑58 (MQ=255) gaagaTGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCAc > 1:704110/1‑59 (MQ=255) gaagaTGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTa > 1:3800940/1‑71 (MQ=255) acggCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTAaaaa < 1:5016134/69‑1 (MQ=255) aTGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgt < 1:3741857/48‑1 (MQ=255) cggCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACaaaa < 1:309817/70‑1 (MQ=255) cggCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACaaaa < 1:1296422/70‑1 (MQ=255) gCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACaaaaaa < 1:386177/71‑1 (MQ=255) gCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACaaaaaa < 1:4788190/71‑1 (MQ=255) cATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCAt < 1:3364544/48‑1 (MQ=255) aTCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACaaaaaaa < 1:2707284/70‑1 (MQ=255) tCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACt > 1:527203/1‑50 (MQ=255) tCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACt > 1:527778/1‑50 (MQ=255) gTCCTGATCGACCAGGATAAATGGCGTGGCTGGCGTATGTGCATCACTGGa > 1:1190904/1‑51 (MQ=255) gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtg > 1:3298731/1‑39 (MQ=255) gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtg > 1:766584/1‑39 (MQ=255) gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtg > 1:1471938/1‑39 (MQ=255) gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTTGATGCCCGTACAAAAAAATCTa > 1:3125095/1‑71 (MQ=255) gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCt > 1:5307946/1‑70 (MQ=255) gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCt > 1:4170749/1‑70 (MQ=255) gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTa < 1:1226672/71‑1 (MQ=255) gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTa < 1:172016/71‑1 (MQ=255) gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTa > 1:2096562/1‑71 (MQ=255) gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTa > 1:3710167/1‑71 (MQ=255) tgcTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACaaaa < 1:2870610/61‑1 (MQ=255) tCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTa < 1:5596636/70‑1 (MQ=255) cTGATTGATCAGGATAAATGCCGTGGCTGGCGTTtgtg < 1:2373668/38‑1 (MQ=25) cTGATTGATCAGGATAAATGCCGTGGCTGGCGTTtgtg < 1:5369587/38‑1 (MQ=25) cTGATTGATCAGGATAAATGCCGTGGCTGGCGTTtgtg < 1:3140944/38‑1 (MQ=25) cTGATTGATCAGGATAAATGCCGTGGCTGGCGTTtgtg < 1:1542559/38‑1 (MQ=25) cTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATc > 1:5600705/1‑66 (MQ=255) cTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACtt < 1:4867959/71‑1 (MQ=255) cTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACtt > 1:363937/1‑71 (MQ=255) cTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGAt > 1:604633/1‑49 (MQ=255) cTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGAt > 1:1489037/1‑49 (MQ=255) cTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACtt > 1:3408598/1‑71 (MQ=255) cTGATCGACCAGGATAAATGACGTGGCTGGCGTAtg > 1:2422380/1‑36 (MQ=255) tGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:4282501/41‑1 (MQ=255) tGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:5099237/41‑1 (MQ=255) tGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:4979727/41‑1 (MQ=255) tGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATc < 1:3647417/65‑1 (MQ=255) tGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATc < 1:259987/65‑1 (MQ=255) gATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCa > 1:1703642/1‑38 (MQ=25) gATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACaaaaaa > 1:4017352/1‑61 (MQ=255) gATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACaaaaaa > 1:1618407/1‑61 (MQ=255) aTCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTc < 1:5626474/69‑1 (MQ=255) cGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACt > 1:2304528/1‑40 (MQ=255) cGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACt > 1:2126145/1‑40 (MQ=255) cAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGa > 1:5192835/1‑70 (MQ=255) cAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGa > 1:5075210/1‑70 (MQ=255) cAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGa > 1:4951460/1‑70 (MQ=255) cAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTgg > 1:3114638/1‑69 (MQ=255) cAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGaa < 1:2101557/71‑1 (MQ=255) aGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGaa > 1:1516311/1‑70 (MQ=255) ggATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAaga < 1:1550963/71‑1 (MQ=255) aTAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTc < 1:408491/59‑1 (MQ=255) aTAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTc < 1:3073298/59‑1 (MQ=255) tAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGc < 1:4141719/70‑1 (MQ=255) tAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCg < 1:5317603/71‑1 (MQ=255) aaTGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCggc < 1:768488/71‑2 (MQ=255) aTGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATc > 1:4430594/1‑49 (MQ=255) tGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACaaaaaa > 1:5000875/1‑45 (MQ=255) ccGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGt > 1:1074032/1‑71 (MQ=255) gTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCt < 1:1570587/71‑1 (MQ=255) ggctggcGTATGTGCATCACTGGATGCCCGTACAAAAAAAt > 1:3078671/1‑41 (MQ=255) ggctggcGTATGTGCATCACTGGATGCCCGTACAAAAAAAt > 1:769644/1‑41 (MQ=255) ggctggcGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAaga < 1:854765/58‑1 (MQ=255) tctggcGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAAt > 1:468652/2‑40 (MQ=12) ctggcGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAgaaa < 1:3476060/71‑3 (MQ=255) ctggcGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAgaaa < 1:1918336/71‑3 (MQ=255) gcGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTa < 1:5520125/59‑1 (MQ=255) cGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTg > 1:5184138/1‑71 (MQ=255) tATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGc < 1:1566502/70‑1 (MQ=255) | GGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGC > minE/835877‑836014 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |