| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 836,008 | G→A | 31.6% | E242E (GAG→GAA) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 836,008 | 0 | G | A | 31.6% | 120.8 / 73.1 | 95 | E242E (GAG→GAA) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base G (33/32); new base A (22/8); total (55/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.60e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
CGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGT > minE/835943‑836077 | cGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTg > 1:5184138/1‑71 (MQ=255) tATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGc < 1:1566502/70‑1 (MQ=255) ttgtgCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCAt > 1:2078164/2‑71 (MQ=255) tgtgCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAg > 1:1521174/1‑65 (MQ=255) tgtgCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAg < 1:1032385/65‑1 (MQ=255) tgtgCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAg > 1:1602462/1‑65 (MQ=255) tgtgCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAg > 1:4618052/1‑65 (MQ=255) tgtgCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCAtc > 1:2845228/1‑71 (MQ=255) tgtgCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCAt < 1:1071002/70‑1 (MQ=255) tgCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGTTAAGTCTGAGAAGTGCAtct < 1:573285/70‑1 (MQ=255) tgCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAtct > 1:1468494/1‑70 (MQ=255) cATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAtcttct < 1:1729687/71‑1 (MQ=255) tCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTg < 1:4083360/70‑1 (MQ=255) tCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGc < 1:4894376/71‑1 (MQ=255) cACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGc > 1:4567232/1‑70 (MQ=255) aCTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTa > 1:1327085/1‑71 (MQ=255) aCTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTa > 1:4679511/1‑71 (MQ=255) tGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCt < 1:1762226/68‑1 (MQ=255) ggATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTAt > 1:1794705/1‑69 (MQ=255) ggATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATcc > 1:563713/1‑71 (MQ=255) gtccGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTACCcacg > 1:3361669/3‑70 (MQ=255) gtccGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTACCcacg > 1:5212552/3‑70 (MQ=255) gtccGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTACCcacg > 1:4130164/3‑70 (MQ=255) gtccGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTACCcacg > 1:1200793/3‑70 (MQ=255) gtccGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTACCcacg > 1:1697622/3‑70 (MQ=255) gtccGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTACCcacg > 1:1433109/3‑70 (MQ=255) tccGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGc > 1:146300/2‑52 (MQ=255) tccGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGc > 1:439229/2‑52 (MQ=255) tccGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTACCcacga < 1:5500711/69‑2 (MQ=255) tccGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTACCcacga < 1:4161664/69‑2 (MQ=255) cGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTACCCACGAAtt < 1:2113350/71‑1 (MQ=255) gTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTgaga < 1:420468/44‑1 (MQ=255) tACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAtgtg > 1:2158642/1‑47 (MQ=255) tACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTg > 1:573306/1‑47 (MQ=255) tACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTg > 1:3215381/1‑47 (MQ=255) tACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGc < 1:4130008/48‑1 (MQ=255) tACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCcgcg < 1:3612143/65‑1 (MQ=255) tACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTAt > 1:5108599/1‑68 (MQ=255) tACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTAt > 1:1815495/1‑68 (MQ=255) tACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGa < 1:2635482/71‑1 (MQ=255) aCAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCcgcg < 1:3179711/64‑1 (MQ=255) cAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAt > 1:5326631/1‑48 (MQ=255) cAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAt > 1:3184983/1‑48 (MQ=255) cAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGaa < 1:2470981/70‑1 (MQ=255) cAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAg > 1:975136/1‑71 (MQ=255) cAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGc > 1:2391424/1‑46 (MQ=255) cAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGc > 1:5032092/1‑46 (MQ=255) cAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGc > 1:2516933/1‑46 (MQ=255) cAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTa > 1:3127156/1‑57 (MQ=255) aaaaaaTCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTACCcacg < 1:5193543/61‑1 (MQ=255) aaaaaTCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTgt > 1:5508231/1‑51 (MQ=255) aaaaTCTACTTCAACTGGAAGAGCGGTAAGTCTGAGaa > 1:4816670/1‑38 (MQ=255) aaaaTCTACTTCAACTGGAAGAGCGGTAAGTCTGAGaa > 1:2843613/1‑38 (MQ=255) aaaaTCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCggg > 1:4372422/1‑71 (MQ=255) aaaTCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGt < 1:1879659/71‑1 (MQ=255) aaaTCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCa > 1:3619306/1‑42 (MQ=19) tACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTACCCACGAAt > 1:892203/1‑56 (MQ=255) aCTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGc > 1:168946/1‑35 (MQ=255) cTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTac > 1:2908237/1‑45 (MQ=255) cTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTACCCACGAATTGagtc > 1:640696/1‑57 (MQ=255) cTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAAcc < 1:3325358/69‑1 (MQ=255) ttCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTa < 1:2808234/44‑1 (MQ=255) ttCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTa < 1:2488452/44‑1 (MQ=255) ttCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTa < 1:4537458/44‑1 (MQ=255) ttCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTAtt < 1:4046841/54‑1 (MQ=255) cAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAtctt > 1:2473592/1‑36 (MQ=255) cAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAg > 1:688396/1‑64 (MQ=255) cAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAg > 1:1748580/1‑64 (MQ=255) cAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGAGGGTCAg > 1:5459720/1‑64 (MQ=255) cAACTGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTacc > 1:218124/1‑42 (MQ=16) aaCTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCg < 1:1274678/70‑1 (MQ=255) aCTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCcg < 1:5354137/44‑1 (MQ=255) tGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCTGCGTATTGa < 1:4650891/50‑1 (MQ=255) tGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGa < 1:42145/50‑1 (MQ=255) tGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGa < 1:566801/50‑1 (MQ=255) tGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGa < 1:1284842/50‑1 (MQ=255) tGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGa < 1:3360763/50‑1 (MQ=255) tGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTACCCACGAAtt < 1:4161880/48‑1 (MQ=255) ggAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAACCGACCGTGTGc > 1:1181434/1‑71 (MQ=255) gAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAg > 1:1831097/1‑50 (MQ=255) gAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAg > 1:3881858/1‑50 (MQ=255) gAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAg > 1:4733433/1‑50 (MQ=255) gAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGc < 1:2373275/70‑1 (MQ=255) gCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGaa < 1:3124529/71‑1 (MQ=255) gCGGCAAGTCAGAAAAATGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAACCGACCGTGTGCTCAGaa < 1:2603101/71‑1 (MQ=255) gTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCg < 1:3512480/57‑1 (MQ=255) tAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGa < 1:4125431/39‑1 (MQ=255) tAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGTGTCAGCCGACCGTGTGCTCAGAAACCt < 1:3052700/71‑1 (MQ=255) aGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCg > 1:807190/1‑41 (MQ=255) aGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCtgt > 1:1209816/1‑71 (MQ=255) aGTCAGAAAAATGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAACCGACCGTGTGCTCAGAAACCtgc > 1:1711302/1‑70 (MQ=255) tCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGc < 1:3714398/57‑1 (MQ=255) tCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGc < 1:3871235/57‑1 (MQ=255) tCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGc < 1:4415156/57‑1 (MQ=255) tGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCgg > 1:4145982/1‑38 (MQ=255) tGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCgg > 1:3008409/1‑38 (MQ=255) agaAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTgtcggt < 1:4389993/71‑1 (MQ=255) agaAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTgtcgg < 1:508103/70‑1 (MQ=255) | CGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGT > minE/835943‑836077 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |