Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 836,362 T→A 36.0% S360S (TCT→TCA narH → nitrate reductase 1, beta (Fe‑S) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE836,3620TA36.0% 48.6 / 49.1 50S360S (TCT→TCAnarHnitrate reductase 1, beta (Fe‑S) subunit
Reads supporting (aligned to +/- strand):  ref base T (19/13);  new base A (16/2);  total (35/15)
Fisher's exact test for biased strand distribution p-value = 5.17e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

GAAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGA  >  minE/836293‑836424
                                                                     |                                                              
gaaACTGGCGCTGCCGCTGCATCCGGAATATCGCACTCTGCCGATGGTCTGGTACGTGCCGCCTCTGtctc                                                               >  1:898755/1‑71 (MQ=255)
gaaACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGtctc                                                               >  1:1106516/1‑71 (MQ=255)
  aaCTGGCGCTGCCGCTGCATCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGa                                                            >  1:1745074/1‑71 (MQ=255)
   aCTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGa                                                            >  1:3532809/1‑71 (MQ=255)
   aCTGGCACTACCGTTGCACCCTGAATACCGCACCCTGCCGATGGTCTGGTACGTTCCTCCGCTGTCACCGa                                                            >  1:2629192/1‑71 (MQ=255)
    cTGGCACTACCGTTGCACCCTGAATACCGCACCCTGCCGATGGTCTGGTACGTTCCTCCGCTGTCACCGAt                                                           >  1:3961595/1‑71 (MQ=255)
       gcgcTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTc                                                         <  1:3956426/70‑1 (MQ=255)
          ctgccgctgcATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTc                                                     <  1:798081/71‑1 (MQ=255)
          ctgccgctgcATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTc                                                     <  1:1295124/71‑1 (MQ=255)
          ctaccgttgcACCCTGAATACCGCACCCTGCCGATGGTCTGGTACGTTCCTCCGCTGTCACCGATTCAGTc                                                     <  1:3645200/68‑1 (MQ=255)
            accgttgcACCCTGAATACCGCACCCTGCCGATGGTCTGGTACGTTCCTCCGCTGTCACCGATTCAGTcc                                                    >  1:5270579/2‑69 (MQ=255)
            accgttgcACCCTGAATACCGCACCCTGCCGATGGTCTGGTACGTTCCTCCGCTGTCACCGATTCAGTcc                                                    >  1:3151648/2‑69 (MQ=255)
             ccgttgcACCCTGAATACCGCACCCTGCCGATGGTCTGGTACGTTCCTCCGCTGTCACCGATTCAGTccta                                                  >  1:1586877/1‑68 (MQ=255)
             ccgttgcACCCTGAATACCGCACCCTGCCGATGGTCTGGTACGTTCCTCCGCTGTCACCGATTCAGTccta                                                  >  1:1899481/1‑68 (MQ=255)
               gctgcATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTgcag                                                >  1:5023683/1‑71 (MQ=255)
                  gcATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTgcag                                                >  1:2268040/1‑68 (MQ=255)
                  gcATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTgcag                                                >  1:5697420/1‑68 (MQ=255)
                    aTCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGAc                                           <  1:1530561/71‑1 (MQ=255)
                      ccGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGAcgc                                         >  1:5116879/1‑71 (MQ=255)
                      ccGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGAcgc                                         >  1:4999635/1‑71 (MQ=255)
                       cGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGAcgcg                                        >  1:4769901/1‑71 (MQ=255)
                         gAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGAcg                                          >  1:1222459/1‑67 (MQ=255)
                         gAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGAcg                                          >  1:1221790/1‑67 (MQ=255)
                         gAATACCGCACCCTGCCGATGGTCTGGTACGTTCCTCCGCTGTCACCGATTCAGTCCTACGCAGATGCggg                                      >  1:3054384/1‑71 (MQ=255)
                                gCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCt                                <  1:2644087/70‑1 (MQ=255)
                                gCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCt                                >  1:4586144/1‑70 (MQ=255)
                                gCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTg                               >  1:4592256/1‑71 (MQ=255)
                                   cccTGCCGATGGTCTGGTACGTTCCTCCGCTGTCACCGAtt                                                          <  1:21667/39‑1 (MQ=19)
                                     cTGCCGATGGTCTGGTACGTTCCTCCGCTGTCACCGATTCa                                                        >  1:2470089/1‑41 (MQ=255)
                                     cTGCCGATGGTCTGGTACGTTCCTCCGCTGTCACCGATTCa                                                        >  1:1838256/1‑41 (MQ=255)
                                     cTGCCGATGGTCTGGTACGTTCCTCCGCTGTCACCGATTCa                                                        >  1:641615/1‑41 (MQ=255)
                                     cTGCCGATGGTCTGGTACGTTCCTCCGCTGTCACCGATTCa                                                        >  1:1628605/1‑41 (MQ=255)
                                     cTGCCGATGGTCTGGTACGTTCCTCCGCTGTCACCGATTCa                                                        >  1:3991079/1‑41 (MQ=255)
                                     cTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGAcgc                                         <  1:1046456/56‑1 (MQ=255)
                                      tGCCGATGGTCTGGTACGTTCCTCCGCTGTCACCGATTCAGt                                                      >  1:1442418/1‑42 (MQ=255)
                                      tGCCGATGGTCTGGTACGTTCCTCCGCTGTCACCGATTCAGTc                                                     >  1:3977702/1‑43 (MQ=255)
                                      tGCCGATGGTCTGGTACGTTCCTCCGCTGTCACCGATTCAGTc                                                     >  1:3143858/1‑43 (MQ=255)
                                       gCCGATGGTCTGGTACGTTCCTCCGCTGTCACCGATTCa                                                        >  1:3657054/1‑39 (MQ=25)
                                        ccGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCaa                       >  1:592186/1‑71 (MQ=255)
                                              gTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGACGCgg                                       <  1:3494288/49‑1 (MQ=255)
                                                 tGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCt              >  1:3697889/1‑71 (MQ=255)
                                                 tGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCt              >  1:1209312/1‑71 (MQ=255)
                                                   gTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGc            <  1:1394618/71‑1 (MQ=255)
                                                      cGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCa                  <  1:1512568/62‑1 (MQ=255)
                                                        tGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGAc                                           <  1:40820/35‑1 (MQ=255)
                                                          ccgccTCTGTCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGt     <  1:2813391/71‑1 (MQ=255)
                                                           cgccTCTGTCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTc    >  1:3174540/1‑71 (MQ=255)
                                                            gccTCTGTCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTc    >  1:5416584/1‑70 (MQ=255)
                                                            gccTCTGTCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTc    >  1:4489950/1‑70 (MQ=255)
                                                             ccTCTGTCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCg   <  1:4985548/70‑1 (MQ=255)
                                                               tctGTCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGt                            <  1:1116983/43‑1 (MQ=255)
                                                               tctGTCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGa  >  1:3588407/1‑69 (MQ=255)
                                                                     tcCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAAc                      >  1:523614/1‑43 (MQ=255)
                                                                     |                                                              
GAAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGA  >  minE/836293‑836424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: