| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 836,446 | G→A | 31.6% | Q388Q (CAG→CAA) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 836,446 | 0 | G | A | 31.6% | 76.1 / 41.4 | 57 | Q388Q (CAG→CAA) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base G (21/18); new base A (6/12); total (27/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01 | |||||||||||
AGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCT > minE/836380‑836511 | agACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATc > 1:1267636/1‑71 (MQ=255)agACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATc > 1:2603199/1‑71 (MQ=255)agACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATc > 1:57277/1‑71 (MQ=255) gACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCt < 1:56326/71‑1 (MQ=255) gACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCt < 1:5278793/71‑1 (MQ=255) tGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATc < 1:3902555/71‑1 (MQ=255) tGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATc < 1:4638752/71‑1 (MQ=255) ggTAGCAACGGCATTCTGCCAGACGTCGAATGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGAc > 1:2362281/1‑71 (MQ=255) ggTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGa < 1:423820/70‑1 (MQ=255) ggTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGa < 1:2075581/70‑1 (MQ=255) ggTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGAc > 1:5250598/1‑71 (MQ=255) gTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGAcc > 1:1523098/1‑71 (MQ=255) gCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGAccgcc > 1:2224747/1‑71 (MQ=255) aaCGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGa < 1:2640002/55‑1 (MQ=255) aaCGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGa < 1:136734/55‑1 (MQ=255) aaCGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGa < 1:5342355/55‑1 (MQ=255) aaCGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCgg > 1:3747422/1‑71 (MQ=255) aCGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATc < 1:1697224/57‑1 (MQ=255) aCGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGt < 1:1931873/71‑1 (MQ=255) aCGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGt > 1:28786/1‑71 (MQ=255) tCTGCCAGACGTCAAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATAcc > 1:3861722/1‑70 (MQ=255) tGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCaaa > 1:2621459/1‑71 (MQ=255) aGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCgg < 1:2195677/71‑1 (MQ=255) gACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGt < 1:386904/71‑1 (MQ=255) gTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACt > 1:2060746/1‑71 (MQ=255) gTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACt > 1:2737099/1‑71 (MQ=255) tCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCaa < 1:2988772/37‑1 (MQ=21) tCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCaa < 1:1288919/37‑1 (MQ=21) tCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCaa < 1:5196247/37‑1 (MQ=21) tCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCaa < 1:210936/37‑1 (MQ=21) tCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCaa < 1:5113463/37‑1 (MQ=21) tCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATatgtt < 1:5129562/43‑3 (MQ=16) tCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGag > 1:5576633/1‑45 (MQ=16) tCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCggc < 1:4324141/53‑2 (MQ=255) tCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCggc < 1:4330503/53‑2 (MQ=255) cGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTgc > 1:3698793/1‑71 (MQ=255) cGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTgc > 1:3473738/1‑71 (MQ=255) gAAAGCCTGCGTATTCCGGTGCAATATCTCGCCaa > 1:3710952/1‑35 (MQ=16) gAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTa > 1:1249682/1‑66 (MQ=255) aGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTgcgcg > 1:1105263/1‑70 (MQ=255) ccTGAGTATTCCGGTACAGTATCTGGCGAATCTGCTGa > 1:3340767/1‑38 (MQ=255) cTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCt < 1:2254244/71‑1 (MQ=255) cTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACt < 1:2687358/71‑1 (MQ=255) cGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGa < 1:4432431/70‑1 (MQ=255) cGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGaa < 1:4110428/71‑1 (MQ=255) cGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa > 1:3630906/1‑70 (MQ=255) gTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGaa > 1:1837881/1‑70 (MQ=255) tATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCt < 1:1188187/66‑1 (MQ=255) tATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACt < 1:1475079/57‑1 (MQ=255) ttCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACg > 1:401318/1‑70 (MQ=255) ttCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACg < 1:1596027/70‑1 (MQ=255) tCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTa > 1:65595/1‑71 (MQ=255) ccGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGAt > 1:1989129/1‑71 (MQ=255) ccGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTAt > 1:4710526/1‑71 (MQ=255) ccGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTAt > 1:1161640/1‑71 (MQ=255) gTACAGTATCTGGCGAATCTGCTGACCGCCGGAGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCt < 1:537934/71‑1 (MQ=255) aCAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCt < 1:1362446/69‑1 (MQ=255) gTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCa > 1:4812093/1‑52 (MQ=255) gTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCa > 1:2672883/1‑52 (MQ=255) gTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCa > 1:603611/1‑52 (MQ=255) | AGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCT > minE/836380‑836511 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |