| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 836,462 | C→T | 25.4% | L394L (CTG→TTG) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 836,462 | 0 | C | T | 25.4% | 91.0 / 29.0 | 55 | L394L (CTG→TTG) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base C (24/17); new base T (8/6); total (32/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACG > minE/836396‑836532 | ggTAGCAACGGCATTCTGCCAGACGTCGAATGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGAc > 1:2362281/1‑71 (MQ=255)ggTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGa < 1:423820/70‑1 (MQ=255)ggTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGa < 1:2075581/70‑1 (MQ=255)ggTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGAc > 1:5250598/1‑71 (MQ=255) gTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGAcc > 1:1523098/1‑71 (MQ=255) gCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGAccgcc > 1:2224747/1‑71 (MQ=255) aaCGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCgg > 1:3747422/1‑71 (MQ=255) aCGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGt > 1:28786/1‑71 (MQ=255) aCGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGt < 1:1931873/71‑1 (MQ=255) tCTGCCAGACGTCAAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATAcc > 1:3861722/1‑70 (MQ=255) tGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCaaa > 1:2621459/1‑71 (MQ=255) aGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCgg < 1:2195677/71‑1 (MQ=255) gACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGt < 1:386904/71‑1 (MQ=255) gTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACt > 1:2060746/1‑71 (MQ=255) gTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACt > 1:2737099/1‑71 (MQ=255) tCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGag > 1:5576633/1‑45 (MQ=16) tCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCggc < 1:4330503/53‑2 (MQ=255) tCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCggc < 1:4324141/53‑2 (MQ=255) cGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTgc > 1:3698793/1‑71 (MQ=255) cGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTgc > 1:3473738/1‑71 (MQ=255) gAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTa > 1:1249682/1‑66 (MQ=255) aGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTgcgcg > 1:1105263/1‑70 (MQ=255) ccTGAGTATTCCGGTACAGTATCTGGCGAATCTGCTGa > 1:3340767/1‑38 (MQ=255) cTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCt < 1:2254244/71‑1 (MQ=255) cTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACt < 1:2687358/71‑1 (MQ=255) cGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGa < 1:4432431/70‑1 (MQ=255) cGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGaa < 1:4110428/71‑1 (MQ=255) cGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa > 1:3630906/1‑70 (MQ=255) gTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGaa > 1:1837881/1‑70 (MQ=255) tATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCt < 1:1188187/66‑1 (MQ=255) tATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACt < 1:1475079/57‑1 (MQ=255) ttCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACg > 1:401318/1‑70 (MQ=255) ttCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACg < 1:1596027/70‑1 (MQ=255) tCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTa > 1:65595/1‑71 (MQ=255) ccGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGAt > 1:1989129/1‑71 (MQ=255) ccGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTAt > 1:4710526/1‑71 (MQ=255) ccGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTAt > 1:1161640/1‑71 (MQ=255) gTACAGTATCTGGCGAATCTGCTGACCGCCGGAGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCt < 1:537934/71‑1 (MQ=255) aCAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCt < 1:1362446/69‑1 (MQ=255) caatATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGc > 1:982427/4‑71 (MQ=255) aatATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGc > 1:5160913/3‑70 (MQ=255) gTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCa > 1:603611/1‑52 (MQ=255) gTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCa > 1:4812093/1‑52 (MQ=255) gTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCa > 1:2672883/1‑52 (MQ=255) tATCTGGCGAATCTGCTGACCGCCGGTGATACCAAAc < 1:4700042/37‑1 (MQ=255) tATCTGGCGAATCTGCTGACCGCCGGTGATACCAAAc < 1:3942951/37‑1 (MQ=255) tATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTa > 1:5315952/1‑42 (MQ=255) aTCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa > 1:236465/1‑54 (MQ=255) aTCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGCGATg > 1:295549/1‑71 (MQ=255) tCTGGCGAATCTGCTGACCGCCGGTGATACCAAAc < 1:1542139/35‑1 (MQ=255) tGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa < 1:1838486/51‑1 (MQ=255) tGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa < 1:1812489/51‑1 (MQ=255) tGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa < 1:727777/51‑1 (MQ=255) aaTCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGaaa > 1:5265714/1‑48 (MQ=255) aaTCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa < 1:1351984/46‑1 (MQ=255) tgctgACCGCCGGTGATACCAAACCGGTACTGCTCGCACTGa < 1:4878885/42‑1 (MQ=255) tgctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa < 1:4524877/42‑1 (MQ=255) tgctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa < 1:2862653/42‑1 (MQ=255) ctgACCGCCGGTTATACCAAACCGGTACTGCGCGCACTGAAACGTa > 1:4896566/1‑46 (MQ=255) ctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTa > 1:5451852/1‑46 (MQ=255) ctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAAc < 1:3179718/70‑1 (MQ=255) ctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACg > 1:563775/1‑71 (MQ=255) | GGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACG > minE/836396‑836532 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |