Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 836,462 C→T 25.4% L394L (CTG→TTG)  narH → nitrate reductase 1, beta (Fe‑S) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE836,4620CT25.4% 91.0 / 29.0 55L394L (CTG→TTG) narHnitrate reductase 1, beta (Fe‑S) subunit
Reads supporting (aligned to +/- strand):  ref base C (24/17);  new base T (8/6);  total (32/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACG  >  minE/836396‑836532
                                                                  |                                                                      
ggTAGCAACGGCATTCTGCCAGACGTCGAATGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGAc                                                                    >  1:2362281/1‑71 (MQ=255)
ggTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGa                                                                     <  1:423820/70‑1 (MQ=255)
ggTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGa                                                                     <  1:2075581/70‑1 (MQ=255)
ggTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGAc                                                                    >  1:5250598/1‑71 (MQ=255)
 gTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGAcc                                                                   >  1:1523098/1‑71 (MQ=255)
    gCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGAccgcc                                                                >  1:2224747/1‑71 (MQ=255)
      aaCGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCgg                                                              >  1:3747422/1‑71 (MQ=255)
       aCGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGt                                                             >  1:28786/1‑71 (MQ=255)
       aCGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGt                                                             <  1:1931873/71‑1 (MQ=255)
              tCTGCCAGACGTCAAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATAcc                                                       >  1:3861722/1‑70 (MQ=255)
                tGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCaaa                                                    >  1:2621459/1‑71 (MQ=255)
                    aGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCgg                                                <  1:2195677/71‑1 (MQ=255)
                     gACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGt                                               <  1:386904/71‑1 (MQ=255)
                        gTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACt                                            >  1:2060746/1‑71 (MQ=255)
                        gTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACt                                            >  1:2737099/1‑71 (MQ=255)
                         tCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGag                                                                    >  1:5576633/1‑45 (MQ=16)
                         tCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCggc                                                             <  1:4330503/53‑2 (MQ=255)
                         tCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCggc                                                             <  1:4324141/53‑2 (MQ=255)
                          cGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTgc                                          >  1:3698793/1‑71 (MQ=255)
                          cGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTgc                                          >  1:3473738/1‑71 (MQ=255)
                           gAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTa                                              >  1:1249682/1‑66 (MQ=255)
                              aGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTgcgcg                                       >  1:1105263/1‑70 (MQ=255)
                                ccTGAGTATTCCGGTACAGTATCTGGCGAATCTGCTGa                                                                     >  1:3340767/1‑38 (MQ=255)
                                 cTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCt                                   <  1:2254244/71‑1 (MQ=255)
                                 cTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACt                                   <  1:2687358/71‑1 (MQ=255)
                                    cGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGa                                 <  1:4432431/70‑1 (MQ=255)
                                    cGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGaa                                <  1:4110428/71‑1 (MQ=255)
                                    cGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa                                 >  1:3630906/1‑70 (MQ=255)
                                     gTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGaa                                >  1:1837881/1‑70 (MQ=255)
                                      tATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCt                                   <  1:1188187/66‑1 (MQ=255)
                                      tATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACt                                            <  1:1475079/57‑1 (MQ=255)
                                        ttCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACg                             >  1:401318/1‑70 (MQ=255)
                                        ttCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACg                             <  1:1596027/70‑1 (MQ=255)
                                         tCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTa                           >  1:65595/1‑71 (MQ=255)
                                          ccGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGAt                          >  1:1989129/1‑71 (MQ=255)
                                          ccGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTAt                          >  1:4710526/1‑71 (MQ=255)
                                          ccGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTAt                          >  1:1161640/1‑71 (MQ=255)
                                             gTACAGTATCTGGCGAATCTGCTGACCGCCGGAGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCt                       <  1:537934/71‑1 (MQ=255)
                                               aCAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCt                       <  1:1362446/69‑1 (MQ=255)
                                                caatATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGc                    >  1:982427/4‑71 (MQ=255)
                                                 aatATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGc                    >  1:5160913/3‑70 (MQ=255)
                                                  gTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCa                                     >  1:603611/1‑52 (MQ=255)
                                                  gTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCa                                     >  1:4812093/1‑52 (MQ=255)
                                                  gTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCa                                     >  1:2672883/1‑52 (MQ=255)
                                                   tATCTGGCGAATCTGCTGACCGCCGGTGATACCAAAc                                                   <  1:4700042/37‑1 (MQ=255)
                                                   tATCTGGCGAATCTGCTGACCGCCGGTGATACCAAAc                                                   <  1:3942951/37‑1 (MQ=255)
                                                   tATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTa                                              >  1:5315952/1‑42 (MQ=255)
                                                    aTCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa                                 >  1:236465/1‑54 (MQ=255)
                                                    aTCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGCGATg                >  1:295549/1‑71 (MQ=255)
                                                     tCTGGCGAATCTGCTGACCGCCGGTGATACCAAAc                                                   <  1:1542139/35‑1 (MQ=255)
                                                       tGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa                                 <  1:1838486/51‑1 (MQ=255)
                                                       tGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa                                 <  1:1812489/51‑1 (MQ=255)
                                                       tGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa                                 <  1:727777/51‑1 (MQ=255)
                                                            aaTCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGaaa                               >  1:5265714/1‑48 (MQ=255)
                                                            aaTCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa                                 <  1:1351984/46‑1 (MQ=255)
                                                                tgctgACCGCCGGTGATACCAAACCGGTACTGCTCGCACTGa                                 <  1:4878885/42‑1 (MQ=255)
                                                                tgctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa                                 <  1:4524877/42‑1 (MQ=255)
                                                                tgctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa                                 <  1:2862653/42‑1 (MQ=255)
                                                                  ctgACCGCCGGTTATACCAAACCGGTACTGCGCGCACTGAAACGTa                           >  1:4896566/1‑46 (MQ=255)
                                                                  ctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTa                           >  1:5451852/1‑46 (MQ=255)
                                                                  ctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAAc   <  1:3179718/70‑1 (MQ=255)
                                                                  ctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACg  >  1:563775/1‑71 (MQ=255)
                                                                  |                                                                      
GGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACG  >  minE/836396‑836532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: