| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 836,481 | A→G | 18.4% | K400R (AAA→AGA) ‡ | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 836,481 | 0 | A | G | 18.4% | 131.9 / 23.7 | 65 | K400R (AAA→AGA) ‡ | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base A (30/23); new base G (7/5); total (37/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.98e-01 | |||||||||||
TGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGAC > minE/836412‑836548 | tGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCaaa > 1:2621459/1‑71 (MQ=255) aGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCgg < 1:2195677/71‑1 (MQ=255) gACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGt < 1:386904/71‑1 (MQ=255) gTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACt > 1:2737099/1‑71 (MQ=255) gTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACt > 1:2060746/1‑71 (MQ=255) cGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTgc > 1:3698793/1‑71 (MQ=255) cGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTgc > 1:3473738/1‑71 (MQ=255) gAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTa > 1:1249682/1‑66 (MQ=255) aGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTgcgcg > 1:1105263/1‑70 (MQ=255) cTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCt < 1:2254244/71‑1 (MQ=255) cTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACt < 1:2687358/71‑1 (MQ=255) cGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGa < 1:4432431/70‑1 (MQ=255) cGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGaa < 1:4110428/71‑1 (MQ=255) cGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa > 1:3630906/1‑70 (MQ=255) gTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGaa > 1:1837881/1‑70 (MQ=255) tATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCt < 1:1188187/66‑1 (MQ=255) tATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACt < 1:1475079/57‑1 (MQ=255) ttCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACg > 1:401318/1‑70 (MQ=255) ttCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACg < 1:1596027/70‑1 (MQ=255) tCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTa > 1:65595/1‑71 (MQ=255) ccGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGAt > 1:1989129/1‑71 (MQ=255) ccGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTAt > 1:1161640/1‑71 (MQ=255) ccGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTAt > 1:4710526/1‑71 (MQ=255) gTACAGTATCTGGCGAATCTGCTGACCGCCGGAGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCt < 1:537934/71‑1 (MQ=255) aCAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCt < 1:1362446/69‑1 (MQ=255) caatATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGc > 1:982427/4‑71 (MQ=255) aatATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGc > 1:5160913/3‑70 (MQ=255) gTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCa > 1:2672883/1‑52 (MQ=255) gTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCa > 1:4812093/1‑52 (MQ=255) gTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCa > 1:603611/1‑52 (MQ=255) tATCTGGCGAATCTGCTGACCGCCGGTGATACCAAAc < 1:3942951/37‑1 (MQ=255) tATCTGGCGAATCTGCTGACCGCCGGTGATACCAAAc < 1:4700042/37‑1 (MQ=255) tATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTa > 1:5315952/1‑42 (MQ=255) aTCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa > 1:236465/1‑54 (MQ=255) aTCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGCGATg > 1:295549/1‑71 (MQ=255) tCTGGCGAATCTGCTGACCGCCGGTGATACCAAAc < 1:1542139/35‑1 (MQ=255) tGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa < 1:1812489/51‑1 (MQ=255) tGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa < 1:1838486/51‑1 (MQ=255) tGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa < 1:727777/51‑1 (MQ=255) aaTCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGaaa > 1:5265714/1‑48 (MQ=255) aaTCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa < 1:1351984/46‑1 (MQ=255) tgctgACCGCCGGTGATACCAAACCGGTACTGCTCGCACTGa < 1:4878885/42‑1 (MQ=255) tgctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa < 1:4524877/42‑1 (MQ=255) tgctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa < 1:2862653/42‑1 (MQ=255) ctgACCGCCGGTTATACCAAACCGGTACTGCGCGCACTGAAACGTa > 1:4896566/1‑46 (MQ=255) ctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTa > 1:5451852/1‑46 (MQ=255) ctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAAc < 1:3179718/70‑1 (MQ=255) ctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACg > 1:563775/1‑71 (MQ=255) tgACCGCCGGTGATACCAAACCGGTACTGCGTGCACTGAAACGTATGCTGGCGATGCGTCAt > 1:2935420/1‑62 (MQ=255) tgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCAt > 1:1198279/1‑62 (MQ=255) tgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCAt > 1:2774006/1‑62 (MQ=255) cgccgGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCt > 1:3694536/1‑70 (MQ=255) ccgGTGATACCAAACCGGTACTGCGCGCACTGAAACg < 1:4073761/37‑1 (MQ=255) ccgGTGATACCAAACCGGTACTGCGCGCACTGAAACg < 1:1461203/37‑1 (MQ=255) ccgGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTg < 1:1610253/44‑1 (MQ=255) cgGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGCGATGCGCCact < 1:1111407/56‑3 (MQ=255) gTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAAcc > 1:5142085/1‑71 (MQ=255) tGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTCGCGATGCGTCATTACAAACGTGCTGAAACCg < 1:4679175/71‑1 (MQ=255) gATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGt > 1:3463473/1‑71 (MQ=255) gATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGt > 1:2490242/1‑71 (MQ=255) aTACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGGGATGCGTCATTACAAACGTGCTGAAACCGtt < 1:4286664/71‑1 (MQ=255) aCCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGa > 1:1714345/1‑40 (MQ=255) aCCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGa > 1:200883/1‑71 (MQ=255) ccAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGAc > 1:5016305/1‑71 (MQ=255) ccAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGAc > 1:4891903/1‑71 (MQ=255) ccAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGAc > 1:3452521/1‑71 (MQ=255) aaaCCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCTTTACaaa > 1:1548044/1‑51 (MQ=255) aaaCCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGa < 1:20819/68‑1 (MQ=255) aaaCCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGa < 1:2536256/68‑1 (MQ=255) aaaCCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGa > 1:4140248/1‑68 (MQ=255) | TGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGAC > minE/836412‑836548 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |