| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 836,510 | C→A | 10.8% | L410M (CTG→ATG) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 836,510 | 0 | C | A | 10.8% | 284.1 / 13.4 | 92 | L410M (CTG→ATG) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base C (35/47); new base A (4/6); total (39/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
GTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGAAGAG > minE/836441‑836578 | gTACAGTATCTGGCGAATCTGCTGACCGCCGGAGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCt < 1:537934/71‑1 (MQ=255) aCAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCt < 1:1362446/69‑1 (MQ=255) caatATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGc > 1:982427/4‑71 (MQ=255) aatATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGc > 1:5160913/3‑70 (MQ=255) aTCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGCGATg > 1:295549/1‑71 (MQ=255) ctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAAc < 1:3179718/70‑1 (MQ=255) ctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACg > 1:563775/1‑71 (MQ=255) tgACCGCCGGTGATACCAAACCGGTACTGCGTGCACTGAAACGTATGCTGGCGATGCGTCAt > 1:2935420/1‑62 (MQ=255) tgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCAt > 1:1198279/1‑62 (MQ=255) tgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCAt > 1:2774006/1‑62 (MQ=255) cgccgGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCt > 1:3694536/1‑70 (MQ=255) ccgGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTg < 1:1610253/44‑1 (MQ=255) cgGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGCGATGCGCCact < 1:1111407/56‑3 (MQ=255) gTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAAcc > 1:5142085/1‑71 (MQ=255) tGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTCGCGATGCGTCATTACAAACGTGCTGAAACCg < 1:4679175/71‑1 (MQ=255) gATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGt > 1:3463473/1‑71 (MQ=255) gATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGt > 1:2490242/1‑71 (MQ=255) aTACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGGGATGCGTCATTACAAACGTGCTGAAACCGtt < 1:4286664/71‑1 (MQ=255) aCCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGa > 1:1714345/1‑40 (MQ=255) aCCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGa > 1:200883/1‑71 (MQ=255) ccAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGAc > 1:4891903/1‑71 (MQ=255) ccAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGAc > 1:5016305/1‑71 (MQ=255) ccAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGAc > 1:3452521/1‑71 (MQ=255) aaaCCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCTTTACaaa > 1:1548044/1‑51 (MQ=255) aaaCCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGa < 1:2536256/68‑1 (MQ=255) aaaCCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGa > 1:4140248/1‑68 (MQ=255) aaaCCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGa < 1:20819/68‑1 (MQ=255) gtccGGTACTGCGGGCGCTGAAACGGATGATGGCGATGCGCCACTATATGCGTTCACAAACCGTGGAAggc > 1:3326425/3‑70 (MQ=255) gtccGGTACTGCGGGCGCTGAAACGGATGATGGCGATGCGCCACTATATGCGTTCACAAACCGTGGAAgg < 1:2408245/68‑1 (MQ=255) aCCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGt > 1:4052406/1‑70 (MQ=255) aCCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTa < 1:1353678/71‑1 (MQ=255) ccGGTACTGCGCTCACTTAAACGTATGCTGGCGATGCGTGATTACa < 1:1580587/46‑1 (MQ=38) ccGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTaa < 1:4350344/71‑1 (MQ=255) ccGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTa > 1:5233940/1‑70 (MQ=255) tACTGCGGGCGCTGAAACGGATGATGGCGATGCGCCACTa < 1:2596377/40‑1 (MQ=12) tACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTAc < 1:4777029/41‑1 (MQ=255) tACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTAc < 1:3921070/41‑1 (MQ=255) tACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGtt < 1:534458/71‑1 (MQ=255) tACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGtt < 1:1352645/71‑1 (MQ=255) tACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGtt < 1:981980/71‑1 (MQ=255) tACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGtt < 1:2113090/71‑1 (MQ=255) aCTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGtt < 1:5254307/70‑1 (MQ=255) cTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTg < 1:5259652/46‑1 (MQ=255) cTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTg < 1:2688951/46‑1 (MQ=255) cTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTg < 1:671869/46‑1 (MQ=255) cTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGa < 1:5091374/71‑1 (MQ=255) cTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGa < 1:5137903/71‑1 (MQ=255) cTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGa < 1:3764270/71‑1 (MQ=255) tGCGGGCGCTGAAACGGATGATGGCGATGCGCCACTATATGCGTTCACAAACCGt < 1:2457603/55‑1 (MQ=255) tGCGGGCGCTGAAACGGATGATGGCGATGCGCCACTATATGCGTTCACAAACCGt < 1:4503339/55‑1 (MQ=255) tGCGGGCGCTGAAACGGATGATGGCGATGCGCCACTATATGCGTTCACAAACCGt < 1:4745926/55‑1 (MQ=255) tGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTg < 1:1062416/45‑1 (MQ=255) gcgcgcACTGAAACGTATGCTGGCGATGCGTCATTACaa > 1:4222580/1‑39 (MQ=255) gcgcgcACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCt > 1:3547417/1‑46 (MQ=255) gcgcgcACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCt > 1:4805507/1‑46 (MQ=255) gcgcgcAATGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATa > 1:5299741/1‑71 (MQ=255) cgcgcACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATa > 1:4197050/1‑70 (MQ=255) cgcgcACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATAc > 1:2717866/1‑71 (MQ=255) cTGAAACGTATGCTGTCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTg < 1:3156322/61‑1 (MQ=255) cTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGtt < 1:2870660/48‑1 (MQ=255) cTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGtt < 1:2356256/48‑1 (MQ=255) cTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGtt < 1:2218809/48‑1 (MQ=255) cTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTg < 1:2086649/61‑1 (MQ=255) cTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGa < 1:3262806/62‑1 (MQ=255) cTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGa < 1:2798985/62‑1 (MQ=255) cTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGa < 1:1230990/62‑1 (MQ=255) tGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTTAAACCGTTGACGGTAAAGtt < 1:5454375/59‑1 (MQ=255) tGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGtt < 1:3389093/59‑1 (MQ=255) tGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGtt < 1:2103654/59‑1 (MQ=255) tGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTgcg > 1:1802740/1‑71 (MQ=255) gAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATa > 1:2508319/1‑62 (MQ=255) gAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATa > 1:4124399/1‑62 (MQ=255) gAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTgcgc > 1:4077020/1‑71 (MQ=255) gAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTgcgc > 1:2254745/1‑71 (MQ=255) aaaCGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTaa < 1:4062642/53‑1 (MQ=255) aaaCGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTaa < 1:3286745/53‑1 (MQ=255) aaaCGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGa < 1:1762796/59‑1 (MQ=255) aaaCGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGa > 1:5472843/1‑59 (MQ=255) aaaCGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCt > 1:5257954/1‑71 (MQ=255) aaaCGTATGCTGGCGATGCGTAATTACAAACGTGCTGAAACCGTTGACGGTaa < 1:3407170/53‑1 (MQ=255) cGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGt > 1:5248609/1‑53 (MQ=255) cGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGt > 1:2676723/1‑53 (MQ=255) gTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGaa < 1:4576868/71‑1 (MQ=255) tGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGa < 1:1677112/52‑1 (MQ=255) tGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGa < 1:2508483/52‑1 (MQ=255) tGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGa < 1:3795849/52‑1 (MQ=255) tGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGaa < 1:1645623/68‑1 (MQ=255) tGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGaa < 1:5386644/68‑1 (MQ=255) tGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGaa > 1:3141503/1‑68 (MQ=255) tGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGaa < 1:4291632/68‑1 (MQ=255) tGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGAagag > 1:619894/1‑71 (MQ=255) tGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGAagag > 1:2160778/1‑71 (MQ=255) cTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAg > 1:2451687/1‑46 (MQ=255) | GTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGAAGAG > minE/836441‑836578 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |