Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 836,748 C→G 27.9% T489S (ACC→AGC) ‡ narH → nitrate reductase 1, beta (Fe‑S) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE836,7480CG27.9% 103.9 / 46.0 68T489S (ACC→AGC) ‡narHnitrate reductase 1, beta (Fe‑S) subunit
Reads supporting (aligned to +/- strand):  ref base C (24/25);  new base G (12/7);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.86e-01

GCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCA  >  minE/836678‑836818
                                                                      |                                                                      
gCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATAc                                                                        <  1:4704751/71‑1 (MQ=255)
gCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATAc                                                                        >  1:4960792/1‑71 (MQ=255)
      gAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAAt                                                                   >  1:1311103/1‑70 (MQ=255)
            ttCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCt                                                            >  1:5374951/1‑71 (MQ=255)
            ttCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCt                                                            >  1:1639148/1‑71 (MQ=255)
              cccGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCa                                                                >  1:2921608/1‑65 (MQ=255)
               ccGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAAtat                                                            <  1:36201/68‑3 (MQ=255)
                  gagaAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCaa                                                      <  1:4171227/71‑1 (MQ=255)
                  gagaAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCaa                                                      <  1:1179091/71‑1 (MQ=255)
                   agaAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAAc                                                     <  1:2411600/71‑1 (MQ=255)
                      aaaaTGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGc                                                  >  1:3696734/1‑71 (MQ=255)
                         aTGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCcgt                                               <  1:1872508/71‑1 (MQ=255)
                          tGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCcgtc                                              <  1:5382576/71‑1 (MQ=255)
                          cggCTGCGGTTTTACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCaa                                                      <  1:559492/62‑1 (MQ=255)
                          cggCTGCGGTTTTACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCaa                                                      <  1:455325/62‑1 (MQ=255)
                            gCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCcgtcg                                             >  1:2837936/1‑70 (MQ=255)
                            gCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCcgtcg                                             <  1:166639/70‑1 (MQ=255)
                                cGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTAt                                          <  1:5664473/69‑1 (MQ=255)
                                   ttttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCt                                                            <  1:135681/47‑1 (MQ=12)
                                   ttttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCaa                                                      <  1:503579/53‑1 (MQ=255)
                                   ttttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCaa                                                      <  1:4524248/53‑1 (MQ=255)
                                   ttttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCaa                                                      <  1:1566295/53‑1 (MQ=255)
                                   cTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATg                                     >  1:2882609/1‑71 (MQ=255)
                                   cTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATg                                     >  1:3789776/1‑71 (MQ=255)
                                    tttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGc                                    >  1:5645257/1‑71 (MQ=255)
                                    tttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGc                                    >  1:1247521/1‑71 (MQ=255)
                                     ttACCTTTGGTGATGGCTGCCACGGTTCAGATACCaaa                                                                    >  1:5224641/1‑38 (MQ=255)
                                     ttACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCa                                                                <  1:3268082/42‑1 (MQ=255)
                                     ttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCaac                                                              >  1:2580879/1‑43 (MQ=12)
                                     ttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGt                                                          >  1:3234015/1‑48 (MQ=12)
                                     ttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGa                                       >  1:5199273/1‑67 (MQ=255)
                                     ttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGa                                       >  1:4812277/1‑67 (MQ=255)
                                     ttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGc                                    >  1:1749722/1‑70 (MQ=255)
                                     ttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGc                                    >  1:1399900/1‑70 (MQ=255)
                                       aCCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGCCAt                                 >  1:3829882/1‑71 (MQ=255)
                                        ccTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATc                                >  1:629293/1‑71 (MQ=255)
                                        ccTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGCCAt                                 >  1:2334146/1‑70 (MQ=255)
                                        ccTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGCCAt                                 >  1:890104/1‑70 (MQ=255)
                                         cTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCg                               <  1:4851786/71‑1 (MQ=255)
                                         cTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCg                               <  1:3580117/71‑1 (MQ=255)
                                          tttGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGa                              >  1:4820286/1‑71 (MQ=255)
                                           ttGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTc                                                        <  1:4573182/44‑1 (MQ=255)
                                           ttGGTGATGGCTGACACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGAt                             >  1:4055775/1‑71 (MQ=255)
                                           ttGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGCCATcaa                              >  1:812827/1‑68 (MQ=255)
                                            tGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGCCATcaac                             <  1:1709982/70‑4 (MQ=255)
                                              gTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGAtgt                           <  1:2375236/70‑1 (MQ=255)
                                              gTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGAtgt                           <  1:330699/70‑1 (MQ=255)
                                              gTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGAtgt                           >  1:4309356/1‑70 (MQ=255)
                                                    gCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAg                     >  1:4026836/1‑70 (MQ=255)
                                                     cTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATc                                         <  1:4582762/49‑1 (MQ=255)
                                                     cTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATc                                         <  1:716228/49‑1 (MQ=255)
                                                       gCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGa                                       >  1:2145847/1‑49 (MQ=255)
                                                        ccACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGc                                    >  1:1045964/1‑51 (MQ=255)
                                                        ccACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCaa                  <  1:3848394/69‑1 (MQ=255)
                                                         cACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCaaaa                >  1:2136498/1‑70 (MQ=255)
                                                         cACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAAc               <  1:3058433/71‑1 (MQ=255)
                                                         cACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATCCCATCGATGTGACCAGCaaaa                >  1:128532/1‑70 (MQ=255)
                                                           cGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGc                    >  1:3199538/1‑64 (MQ=255)
                                                           cGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACgg             <  1:2870932/71‑1 (MQ=255)
                                                            ggTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCGGCAAAACGGa            <  1:5431492/71‑1 (MQ=255)
                                                             gTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATc                                <  1:5630494/50‑1 (MQ=255)
                                                              ttCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGa                                       >  1:299193/1‑42 (MQ=255)
                                                                cAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGAtg                            >  1:1454265/1‑51 (MQ=255)
                                                                  gATACCAAATTCAATCTGTTCAACAGCCGTCGTATc                                         <  1:5222642/36‑1 (MQ=255)
                                                                  gATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATc                                <  1:2853566/45‑1 (MQ=255)
                                                                   aTACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATc                                <  1:367954/44‑1 (MQ=255)
                                                                   aTACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATc                                <  1:300315/44‑1 (MQ=255)
                                                                     aCCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGa                              >  1:2860480/1‑44 (MQ=255)
                                                                     aCCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATc    >  1:4385657/1‑70 (MQ=255)
                                                                     aCCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATc    >  1:3407759/1‑70 (MQ=255)
                                                                      ccAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATc                                <  1:3684094/41‑1 (MQ=255)
                                                                      ccAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATc                                <  1:1058943/41‑1 (MQ=255)
                                                                      ccAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATcc   <  1:1749367/70‑1 (MQ=255)
                                                                      ccAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCa  >  1:5092478/1‑71 (MQ=255)
                                                                      ccAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCa  >  1:1329710/1‑71 (MQ=255)
                                                                      |                                                                      
GCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCA  >  minE/836678‑836818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: