| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 836,748 | C→G | 27.9% | T489S (ACC→AGC) ‡ | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 836,748 | 0 | C | G | 27.9% | 103.9 / 46.0 | 68 | T489S (ACC→AGC) ‡ | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base C (24/25); new base G (12/7); total (36/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.86e-01 | |||||||||||
GCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCA > minE/836678‑836818 | gCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATAc < 1:4704751/71‑1 (MQ=255)gCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATAc > 1:4960792/1‑71 (MQ=255) gAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAAt > 1:1311103/1‑70 (MQ=255) ttCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCt > 1:5374951/1‑71 (MQ=255) ttCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCt > 1:1639148/1‑71 (MQ=255) cccGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCa > 1:2921608/1‑65 (MQ=255) ccGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAAtat < 1:36201/68‑3 (MQ=255) gagaAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCaa < 1:4171227/71‑1 (MQ=255) gagaAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCaa < 1:1179091/71‑1 (MQ=255) agaAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAAc < 1:2411600/71‑1 (MQ=255) aaaaTGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGc > 1:3696734/1‑71 (MQ=255) aTGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCcgt < 1:1872508/71‑1 (MQ=255) tGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCcgtc < 1:5382576/71‑1 (MQ=255) cggCTGCGGTTTTACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCaa < 1:559492/62‑1 (MQ=255) cggCTGCGGTTTTACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCaa < 1:455325/62‑1 (MQ=255) gCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCcgtcg > 1:2837936/1‑70 (MQ=255) gCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCcgtcg < 1:166639/70‑1 (MQ=255) cGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTAt < 1:5664473/69‑1 (MQ=255) ttttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCt < 1:135681/47‑1 (MQ=12) ttttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCaa < 1:503579/53‑1 (MQ=255) ttttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCaa < 1:4524248/53‑1 (MQ=255) ttttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCaa < 1:1566295/53‑1 (MQ=255) cTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATg > 1:2882609/1‑71 (MQ=255) cTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATg > 1:3789776/1‑71 (MQ=255) tttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGc > 1:5645257/1‑71 (MQ=255) tttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGc > 1:1247521/1‑71 (MQ=255) ttACCTTTGGTGATGGCTGCCACGGTTCAGATACCaaa > 1:5224641/1‑38 (MQ=255) ttACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCa < 1:3268082/42‑1 (MQ=255) ttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCaac > 1:2580879/1‑43 (MQ=12) ttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGt > 1:3234015/1‑48 (MQ=12) ttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGa > 1:5199273/1‑67 (MQ=255) ttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGa > 1:4812277/1‑67 (MQ=255) ttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGc > 1:1749722/1‑70 (MQ=255) ttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGc > 1:1399900/1‑70 (MQ=255) aCCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGCCAt > 1:3829882/1‑71 (MQ=255) ccTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATc > 1:629293/1‑71 (MQ=255) ccTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGCCAt > 1:2334146/1‑70 (MQ=255) ccTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGCCAt > 1:890104/1‑70 (MQ=255) cTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCg < 1:4851786/71‑1 (MQ=255) cTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCg < 1:3580117/71‑1 (MQ=255) tttGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGa > 1:4820286/1‑71 (MQ=255) ttGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTc < 1:4573182/44‑1 (MQ=255) ttGGTGATGGCTGACACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGAt > 1:4055775/1‑71 (MQ=255) ttGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGCCATcaa > 1:812827/1‑68 (MQ=255) tGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGCCATcaac < 1:1709982/70‑4 (MQ=255) gTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGAtgt < 1:2375236/70‑1 (MQ=255) gTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGAtgt < 1:330699/70‑1 (MQ=255) gTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGAtgt > 1:4309356/1‑70 (MQ=255) gCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAg > 1:4026836/1‑70 (MQ=255) cTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATc < 1:4582762/49‑1 (MQ=255) cTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATc < 1:716228/49‑1 (MQ=255) gCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGa > 1:2145847/1‑49 (MQ=255) ccACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGc > 1:1045964/1‑51 (MQ=255) ccACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCaa < 1:3848394/69‑1 (MQ=255) cACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCaaaa > 1:2136498/1‑70 (MQ=255) cACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAAc < 1:3058433/71‑1 (MQ=255) cACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATCCCATCGATGTGACCAGCaaaa > 1:128532/1‑70 (MQ=255) cGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGc > 1:3199538/1‑64 (MQ=255) cGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACgg < 1:2870932/71‑1 (MQ=255) ggTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCGGCAAAACGGa < 1:5431492/71‑1 (MQ=255) gTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATc < 1:5630494/50‑1 (MQ=255) ttCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGa > 1:299193/1‑42 (MQ=255) cAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGAtg > 1:1454265/1‑51 (MQ=255) gATACCAAATTCAATCTGTTCAACAGCCGTCGTATc < 1:5222642/36‑1 (MQ=255) gATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATc < 1:2853566/45‑1 (MQ=255) aTACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATc < 1:367954/44‑1 (MQ=255) aTACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATc < 1:300315/44‑1 (MQ=255) aCCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGa > 1:2860480/1‑44 (MQ=255) aCCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATc > 1:4385657/1‑70 (MQ=255) aCCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATc > 1:3407759/1‑70 (MQ=255) ccAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATc < 1:3684094/41‑1 (MQ=255) ccAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATc < 1:1058943/41‑1 (MQ=255) ccAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATcc < 1:1749367/70‑1 (MQ=255) ccAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCa > 1:5092478/1‑71 (MQ=255) ccAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCa > 1:1329710/1‑71 (MQ=255) | GCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCA > minE/836678‑836818 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |