| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 836,771 | C→A | 17.1% | R497S (CGT→AGT) ‡ | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 836,771 | 0 | C | A | 17.1% | 199.3 / 17.8 | 75 | R497S (CGT→AGT) ‡ | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base C (29/33); new base A (11/2); total (40/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.54e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGT > minE/836703‑836837 | aTGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCcgt < 1:1872508/71‑1 (MQ=255) tGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCcgtc < 1:5382576/71‑1 (MQ=255) gCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCcgtcg > 1:2837936/1‑70 (MQ=255) gCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCcgtcg < 1:166639/70‑1 (MQ=255) cGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTAt < 1:5664473/69‑1 (MQ=255) cTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATg > 1:2882609/1‑71 (MQ=255) cTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATg > 1:3789776/1‑71 (MQ=255) tttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGc > 1:5645257/1‑71 (MQ=255) tttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGc > 1:1247521/1‑71 (MQ=255) ttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGa > 1:5199273/1‑67 (MQ=255) ttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGa > 1:4812277/1‑67 (MQ=255) ttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGc > 1:1399900/1‑70 (MQ=255) ttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGc > 1:1749722/1‑70 (MQ=255) aCCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGCCAt > 1:3829882/1‑71 (MQ=255) ccTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATc > 1:629293/1‑71 (MQ=255) ccTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGCCAt > 1:890104/1‑70 (MQ=255) ccTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGCCAt > 1:2334146/1‑70 (MQ=255) cTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCg < 1:3580117/71‑1 (MQ=255) cTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCg < 1:4851786/71‑1 (MQ=255) tttGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGa > 1:4820286/1‑71 (MQ=255) ttGGTGATGGCTGACACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGAt > 1:4055775/1‑71 (MQ=255) ttGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGCCATcaa > 1:812827/1‑68 (MQ=255) tGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGCCATcaac < 1:1709982/70‑4 (MQ=255) gTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGAtgt < 1:330699/70‑1 (MQ=255) gTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGAtgt < 1:2375236/70‑1 (MQ=255) gTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGAtgt > 1:4309356/1‑70 (MQ=255) gCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAg > 1:4026836/1‑70 (MQ=255) cTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATc < 1:716228/49‑1 (MQ=255) cTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATc < 1:4582762/49‑1 (MQ=255) gCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGa > 1:2145847/1‑49 (MQ=255) ccACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGc > 1:1045964/1‑51 (MQ=255) ccACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCaa < 1:3848394/69‑1 (MQ=255) cACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCaaaa > 1:2136498/1‑70 (MQ=255) cACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAAc < 1:3058433/71‑1 (MQ=255) cACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATCCCATCGATGTGACCAGCaaaa > 1:128532/1‑70 (MQ=255) cGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGc > 1:3199538/1‑64 (MQ=255) cGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACgg < 1:2870932/71‑1 (MQ=255) ggTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCGGCAAAACGGa < 1:5431492/71‑1 (MQ=255) gTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATc < 1:5630494/50‑1 (MQ=255) ttCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGa > 1:299193/1‑42 (MQ=255) cAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGAtg > 1:1454265/1‑51 (MQ=255) gATACCAAATTCAATCTGTTCAACAGCCGTCGTATc < 1:5222642/36‑1 (MQ=255) gATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATc < 1:2853566/45‑1 (MQ=255) aTACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATc < 1:300315/44‑1 (MQ=255) aTACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATc < 1:367954/44‑1 (MQ=255) aCCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGa > 1:2860480/1‑44 (MQ=255) aCCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATc > 1:3407759/1‑70 (MQ=255) aCCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATc > 1:4385657/1‑70 (MQ=255) ccAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATc < 1:1058943/41‑1 (MQ=255) ccAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATc < 1:3684094/41‑1 (MQ=255) ccAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATcc < 1:1749367/70‑1 (MQ=255) ccAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCa > 1:1329710/1‑71 (MQ=255) ccAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCa > 1:5092478/1‑71 (MQ=255) cAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGAt > 1:3869012/1‑43 (MQ=255) cAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGAt > 1:1751439/1‑43 (MQ=255) cAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGAt > 1:3497106/1‑43 (MQ=255) aaaTTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCa > 1:1214244/1‑65 (MQ=255) aaaTTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATg < 1:3233404/71‑1 (MQ=255) aaaTTCAACCTGTTCAACAGTAGCCGTATCGATGc > 1:5453585/1‑35 (MQ=19) aaTTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAATACgg < 1:4772659/57‑1 (MQ=255) aaTTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACgg < 1:1290494/57‑1 (MQ=255) aaTTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACgg < 1:1474416/57‑1 (MQ=255) aaTTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGa > 1:4456266/1‑71 (MQ=255) aTTCAACCTGTTCAACAGTAGCCGTATCGATGCCATc < 1:2306834/37‑1 (MQ=25) tCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCaaaa > 1:798479/1‑51 (MQ=255) aTCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCaa < 1:5214504/46‑1 (MQ=255) aTCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCaa < 1:1959302/46‑1 (MQ=255) cTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCaaaa > 1:4316314/1‑46 (MQ=255) ttCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGt < 1:5443660/70‑1 (MQ=255) cAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGAt > 1:3510515/1‑71 (MQ=255) aaCAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGAt < 1:3183229/70‑1 (MQ=255) aCAGCCGTCGTATCGATGCCATCGATGTGACCAGCaa < 1:4853204/37‑1 (MQ=255) cAGCCGTCGTATCGATGCCATCGATGTGACCAGCaa < 1:5079524/36‑1 (MQ=255) cAGCCGTCGTATCGATGCCATCGATGTGACCAGCaa < 1:2951740/36‑1 (MQ=255) cAGCCGTCGTATCGATGCCATCGATGTGACCAGCaa < 1:3970209/36‑1 (MQ=255) cAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGc > 1:2238341/1‑47 (MQ=255) cAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGc > 1:2802857/1‑47 (MQ=255) cAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGt < 1:5296764/71‑1 (MQ=255) cAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGt < 1:1541486/71‑1 (MQ=255) | ATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGT > minE/836703‑836837 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |