| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 837,040 | T→C | 23.9% | L75L (TTG→CTG) | narJ → | molybdenum‑cofactor‑assembly chaperone subunit (delta subunit) of nitrate reductase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 837,040 | 0 | T | C | 23.9% | 112.8 / 35.4 | 67 | L75L (TTG→CTG) | narJ | molybdenum‑cofactor‑assembly chaperone subunit (delta subunit) of nitrate reductase 1 |
| Reads supporting (aligned to +/- strand): ref base T (20/31); new base C (8/8); total (28/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
TCCGCTCGATGCCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAG > minE/836979‑837108 | tCCGCTCGATGCCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGttt > 1:1243331/1‑70 (MQ=255)tCCGCTCGATGCCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTg < 1:2925923/71‑1 (MQ=255)tCCGCTCGATGCCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTg < 1:3830050/71‑1 (MQ=255) cGCTCGATGCCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGaa < 1:2634176/71‑1 (MQ=255) cGCTCGATGCCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGaa < 1:4486640/71‑1 (MQ=255) gCTCGATGCCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGaa < 1:3021745/70‑1 (MQ=255) cTCGATGCCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTg < 1:3115401/60‑1 (MQ=255) cTCGATGCCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTg < 1:3730991/60‑1 (MQ=255) cTCGATGCCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGaa < 1:679740/69‑1 (MQ=255) cTCGATGCCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACa < 1:5285151/71‑1 (MQ=255) gATGCCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACAtgt > 1:4743326/1‑71 (MQ=255) cccAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCa < 1:2142867/70‑1 (MQ=255) cccAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCa < 1:5129755/70‑1 (MQ=255) ccAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACg > 1:4304606/1‑71 (MQ=255) ccAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACg < 1:3982208/71‑1 (MQ=255) cAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCAc > 1:1774486/1‑69 (MQ=255) cAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCAc > 1:528943/1‑69 (MQ=255) cAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACgg > 1:4123467/1‑71 (MQ=255) cAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACgg > 1:5328383/1‑71 (MQ=255) ggCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCg < 1:1142602/71‑1 (MQ=255) gcgcAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCg > 1:1429854/1‑70 (MQ=255) gcgcAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCg > 1:1968832/1‑70 (MQ=255) cAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATc < 1:1273041/71‑1 (MQ=255) cAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATc < 1:3038748/71‑1 (MQ=255) tACAGCGAACTGTTCGACCGTGGCCGCTCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCcg > 1:5331585/1‑71 (MQ=255) aCAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCcgc > 1:4351798/1‑71 (MQ=255) cAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCcgc < 1:3180810/70‑1 (MQ=255) cAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCcgc > 1:3275029/1‑70 (MQ=255) cAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCcgc < 1:4655449/70‑1 (MQ=255) cAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCcgc > 1:1954183/1‑70 (MQ=255) gCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTg < 1:5411941/45‑1 (MQ=255) gAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCcgc < 1:3267845/66‑1 (MQ=255) aaCTGTTCGACCGTGGCCGCGCCACGTCACTGTTg < 1:4796508/35‑1 (MQ=255) gTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGt < 1:2861544/35‑1 (MQ=255) ttCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGa > 1:2390564/1‑38 (MQ=255) ttCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGa > 1:3726861/1‑62 (MQ=255) ttCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGa > 1:1313003/1‑62 (MQ=255) gACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGc < 1:347441/71‑1 (MQ=255) gACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGAACGCGGCCAGGc < 1:5569837/71‑1 (MQ=255) aCCGCGGGCGCACCACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGc < 1:248931/70‑1 (MQ=255) ccGCGGGCGCACCACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGcaa < 1:2033718/71‑3 (MQ=255) ccGCGGGCGCACCACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGcaa < 1:4219983/71‑3 (MQ=255) gTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGAtg < 1:3691372/71‑1 (MQ=255) gCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGAtggt < 1:1477056/70‑1 (MQ=255) gcgcaCCACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAAtggtgg < 1:2844976/70‑1 (MQ=255) gcgcaCCACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAAtggtgg < 1:4929275/70‑1 (MQ=255) cgcgCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCg > 1:4555090/1‑40 (MQ=255) ccACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACct > 1:5264352/1‑70 (MQ=255) cACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAAtgttgg < 1:5638980/65‑1 (MQ=255) cACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAAtggtgg < 1:4664664/65‑1 (MQ=255) cACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAAtgg > 1:3892172/1‑62 (MQ=255) cACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACctgc > 1:4472078/1‑71 (MQ=255) cACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACctgc > 1:4174436/1‑71 (MQ=255) cACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACctgc > 1:3472542/1‑71 (MQ=255) cACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACctgc > 1:2444782/1‑71 (MQ=255) cACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGAtggtgg < 1:3683966/65‑1 (MQ=255) aCGTCCCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGcaa > 1:3736215/1‑56 (MQ=255) cGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACctgctg > 1:3029830/1‑71 (MQ=255) gTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGc < 1:5464509/54‑1 (MQ=255) tcgctgctgctgTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGgc < 1:1388791/68‑1 (MQ=255) cgctgctgctgTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGgcg > 1:4414028/3‑71 (MQ=255) gctgctgctgTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAAtg > 1:3958707/2‑55 (MQ=255) gctgctgctgTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGgcg < 1:1021282/69‑1 (MQ=255) gctgctgctgTTCGAACATATTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGgcg < 1:3803419/69‑1 (MQ=255) aCTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGc < 1:900444/51‑1 (MQ=255) aCTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGgcg > 1:4511865/1‑70 (MQ=255) cTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGgcg > 1:955244/1‑69 (MQ=255) tGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGAtggtgg < 1:352813/57‑1 (MQ=255) tGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGAtggtgg < 1:4333687/57‑1 (MQ=255) tGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAg > 1:2359348/1‑71 (MQ=255) gTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGc < 1:2335640/48‑1 (MQ=255) gTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGc < 1:973012/48‑1 (MQ=255) ttGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGAtg > 1:1548587/1‑51 (MQ=255) | TCCGCTCGATGCCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAG > minE/836979‑837108 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |