| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 837,057 | G→T | 38.6% | V80V (GTG→GTT) | narJ → | molybdenum‑cofactor‑assembly chaperone subunit (delta subunit) of nitrate reductase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 837,057 | 0 | G | T | 38.6% | 98.9 / 77.5 | 88 | V80V (GTG→GTT) | narJ | molybdenum‑cofactor‑assembly chaperone subunit (delta subunit) of nitrate reductase 1 |
| Reads supporting (aligned to +/- strand): ref base G (25/29); new base T (17/17); total (42/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.97e-01 | |||||||||||
CCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTG > minE/836990‑837126 | cccAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCa < 1:2142867/70‑1 (MQ=255)cccAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCa < 1:5129755/70‑1 (MQ=255) ccAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACg < 1:3982208/71‑1 (MQ=255) ccAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACg > 1:4304606/1‑71 (MQ=255) cAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCAc > 1:1774486/1‑69 (MQ=255) cAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCAc > 1:528943/1‑69 (MQ=255) cAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACgg > 1:4123467/1‑71 (MQ=255) cAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACgg > 1:5328383/1‑71 (MQ=255) ggCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCg < 1:1142602/71‑1 (MQ=255) gcgcAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCg > 1:1429854/1‑70 (MQ=255) gcgcAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCg > 1:1968832/1‑70 (MQ=255) cAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATc < 1:3038748/71‑1 (MQ=255) cAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATc < 1:1273041/71‑1 (MQ=255) tACAGCGAACTGTTCGACCGTGGCCGCTCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCcg > 1:5331585/1‑71 (MQ=255) aCAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCcgc > 1:4351798/1‑71 (MQ=255) cAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCcgc < 1:4655449/70‑1 (MQ=255) cAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCcgc < 1:3180810/70‑1 (MQ=255) cAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCcgc > 1:3275029/1‑70 (MQ=255) cAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCcgc > 1:1954183/1‑70 (MQ=255) gAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCcgc < 1:3267845/66‑1 (MQ=255) ttCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGa > 1:3726861/1‑62 (MQ=255) ttCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGa > 1:1313003/1‑62 (MQ=255) gACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGc < 1:347441/71‑1 (MQ=255) gACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGAACGCGGCCAGGc < 1:5569837/71‑1 (MQ=255) aCCGCGGGCGCACCACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGc < 1:248931/70‑1 (MQ=255) ccGCGGGCGCACCACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGcaa < 1:4219983/71‑3 (MQ=255) ccGCGGGCGCACCACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGcaa < 1:2033718/71‑3 (MQ=255) gTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGAtg < 1:3691372/71‑1 (MQ=255) gCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGAtggt < 1:1477056/70‑1 (MQ=255) gcgcaCCACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAAtggtgg < 1:4929275/70‑1 (MQ=255) gcgcaCCACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAAtggtgg < 1:2844976/70‑1 (MQ=255) cgcgCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCg > 1:4555090/1‑40 (MQ=255) ccACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACct > 1:5264352/1‑70 (MQ=255) cACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAAtgttgg < 1:5638980/65‑1 (MQ=255) cACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAAtggtgg < 1:4664664/65‑1 (MQ=255) cACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAAtgg > 1:3892172/1‑62 (MQ=255) cACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACctgc > 1:2444782/1‑71 (MQ=255) cACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACctgc > 1:4174436/1‑71 (MQ=255) cACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACctgc > 1:3472542/1‑71 (MQ=255) cACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACctgc > 1:4472078/1‑71 (MQ=255) cACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGAtggtgg < 1:3683966/65‑1 (MQ=255) aCGTCCCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGcaa > 1:3736215/1‑56 (MQ=255) cGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACctgctg > 1:3029830/1‑71 (MQ=255) gTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGc < 1:5464509/54‑1 (MQ=255) tcgctgctgctgTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGgc < 1:1388791/68‑1 (MQ=255) cgctgctgctgTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGgcg > 1:4414028/3‑71 (MQ=255) gctgctgctgTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAAtg > 1:3958707/2‑55 (MQ=255) gctgctgctgTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGgcg < 1:1021282/69‑1 (MQ=255) gctgctgctgTTCGAACATATTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGgcg < 1:3803419/69‑1 (MQ=255) aCTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGc < 1:900444/51‑1 (MQ=255) aCTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGgcg > 1:4511865/1‑70 (MQ=255) cTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGgcg > 1:955244/1‑69 (MQ=255) tGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGAtggtgg < 1:4333687/57‑1 (MQ=255) tGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGAtggtgg < 1:352813/57‑1 (MQ=255) tGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAg > 1:2359348/1‑71 (MQ=255) gctGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGGCGGAg > 1:2021444/3‑70 (MQ=255) gTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGc < 1:973012/48‑1 (MQ=255) gTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGc < 1:2335640/48‑1 (MQ=255) ttGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGAtg > 1:1548587/1‑51 (MQ=255) ctGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACctgc < 1:3437199/60‑1 (MQ=255) ctGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGGCGGAGt < 1:4773093/69‑1 (MQ=255) ctGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGGCGGAGTa < 1:1249391/70‑1 (MQ=255) ctGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGGCGGAGTa > 1:2197833/2‑71 (MQ=255) tGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTgg > 1:2438912/1‑63 (MQ=255) cTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACg < 1:241880/70‑1 (MQ=255) cTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGa < 1:1022181/71‑1 (MQ=255) cTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGcaa < 1:1369561/46‑3 (MQ=16) cTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGGCGGAGTATGa < 1:229261/71‑1 (MQ=255) cTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGGCGGAGTATGa < 1:2408335/71‑1 (MQ=255) gTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGag > 1:386382/1‑70 (MQ=255) gTTTGAACATGTGCACGGCGAATCCCGCGAC‑‑CGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGagcag > 1:2686838/1‑71 (MQ=255) tttGAACTTGTGCACGGCGAATCCCGCGACCGCGGCCAGGc < 1:1025207/41‑1 (MQ=255) tttGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGc < 1:3737028/41‑1 (MQ=255) tttGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACctg < 1:2986800/54‑1 (MQ=255) tttGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGag < 1:2469044/69‑1 (MQ=255) ttGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGa > 1:4370747/1‑42 (MQ=255) ttGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGagca < 1:600224/70‑1 (MQ=255) tGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGagcag < 1:2064394/70‑1 (MQ=255) cgAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAAtggtg > 1:5423722/2‑46 (MQ=16) gAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGa < 1:2838369/65‑1 (MQ=255) tcATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACctgc > 1:719290/2‑50 (MQ=255) aCATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACctgc > 1:4525617/1‑50 (MQ=255) aCATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACctgc > 1:456750/1‑50 (MQ=255) aCATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACctg > 1:2669587/1‑49 (MQ=255) aCATGTTCATGCCGAGTCCAGCGATCGCGGCCAGGCAATGGTGGACctgc > 1:5371076/1‑50 (MQ=255) aCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACctgc > 1:848913/1‑50 (MQ=255) aCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACctgc > 1:644956/1‑50 (MQ=255) aTGTGCACGGCGAATCCCGCGACC‑CGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAgcagca < 1:4636300/66‑1 (MQ=255) tgCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTg < 1:2243148/71‑1 (MQ=255) | CCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTG > minE/836990‑837126 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |