| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 837,705 | C→T | 26.5% | L60L (CTG→TTG) | narI → | nitrate reductase 1, gamma (cytochrome b(NR)) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 837,705 | 0 | C | T | 26.5% | 113.1 / 40.1 | 68 | L60L (CTG→TTG) | narI | nitrate reductase 1, gamma (cytochrome b(NR)) subunit |
| Reads supporting (aligned to +/- strand): ref base C (20/30); new base T (3/15); total (23/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.81e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
GGCGTCCAGCCAGATGCTGGATCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGAT > minE/837638‑837775 | ggcgTCCAGCCAGATGCTGGATCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTg < 1:5327366/70‑1 (MQ=255) ccagccagATGCTGGATCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTc > 1:4871605/1‑63 (MQ=255) cagccagATGCTGGATCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGAttt > 1:3464297/1‑71 (MQ=255) cagccagATGCTGGATCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGAttt > 1:3620679/1‑71 (MQ=255) cagccagATGCTGGATCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGAttt < 1:5195918/71‑1 (MQ=255) cagccagATGCTGGATCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGAttt > 1:2337270/1‑71 (MQ=255) cagccagATGCTGGATCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGAtt < 1:3364014/70‑1 (MQ=255) aTGCTGGATCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCgg < 1:20724/71‑1 (MQ=255) tGCTGGATCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGAttttt > 1:2796961/1‑65 (MQ=255) gCTGGATCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGt > 1:3104546/1‑70 (MQ=255) cTGGATCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTggg < 1:1202644/56‑1 (MQ=255) ggATCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCt > 1:5453522/1‑51 (MQ=255) ggATCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCt > 1:4506375/1‑51 (MQ=255) ggATCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCt > 1:1940993/1‑51 (MQ=255) gATCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCActt < 1:537561/71‑1 (MQ=255) gATCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCAc < 1:753902/69‑1 (MQ=255) aTCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGAttttt < 1:3764596/59‑1 (MQ=255) aTCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGAttttt < 1:3595563/59‑1 (MQ=255) aTCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGAttttt < 1:3333333/59‑1 (MQ=255) tCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCActtct < 1:2740018/71‑1 (MQ=255) tCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCActtct > 1:5613210/1‑71 (MQ=255) cGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGAttt < 1:5295160/55‑1 (MQ=255) gCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCa < 1:3957735/64‑1 (MQ=255) gCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCActtctt < 1:4692638/70‑1 (MQ=255) aaaGGGATGAACCTGGCGTCGAACCTGTTCCATGTCGGGATTCTGGGGAt > 1:4840042/1‑50 (MQ=255) aaaGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCgg < 1:1799919/71‑1 (MQ=255) aGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTa > 1:633968/1‑71 (MQ=255) gggATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCg > 1:4080490/1‑67 (MQ=255) gggATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCg > 1:1547980/1‑67 (MQ=255) gATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTa > 1:1127883/1‑68 (MQ=255) aTGAACCTGGCGTCGAACCTGTTCCATATCGGGATTc < 1:1394125/37‑1 (MQ=255) gAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGa > 1:4804580/1‑71 (MQ=255) ccTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACttcttc < 1:5253222/58‑1 (MQ=255) cTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGAcgcc < 1:1498757/71‑1 (MQ=255) tGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGAcgccg > 1:1827984/1‑71 (MQ=255) tCGAATCTGTTCCATATCGGCATTTTGGGGATTTTCTTCGGGCAc < 1:1052377/45‑1 (MQ=16) tCGAATCTGTTCCATATCGGCATTTTGGGGATTTTCTTCGGGCAc < 1:5684795/45‑1 (MQ=16) tCGAATCTGTTCCATATCGGCATTTTGGGGATTTTCTTCGGGCAc < 1:2456482/45‑1 (MQ=16) tCGAATCTGTTCCATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATGTTAACGCCGCACTg < 1:5667574/71‑1 (MQ=255) gAATCTGTTCCATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATGTTAACGCCGCACTGGa > 1:1772263/1‑71 (MQ=255) aatcTGTTCCATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATGTTAACGCCGCACTGGAt > 1:4149972/4‑71 (MQ=255) atcTGTTCCATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATGTTAACGCCGCACt < 1:4370513/64‑1 (MQ=255) atcTGTTCCATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATGTTAACGCCGCACTGGATg < 1:3530681/69‑1 (MQ=255) atcTGTTCCATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATGTTAACGCCGCACTGGATg < 1:4836129/69‑1 (MQ=255) aCCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTa < 1:3078725/51‑1 (MQ=255) aCCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTa < 1:4547925/51‑1 (MQ=255) tGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTAt > 1:3813579/1‑71 (MQ=255) tGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTAt > 1:3413289/1‑71 (MQ=255) gTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGt < 1:1693949/68‑1 (MQ=255) ttCCATATCGGGATTCTGGGGATTTTTGTCGGTCActtctt > 1:2709699/1‑41 (MQ=255) ttCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCgg < 1:3601887/44‑1 (MQ=255) ttCCATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCgg < 1:2088660/44‑1 (MQ=255) ttCCATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCAt < 1:1660383/47‑1 (MQ=16) ttCCATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCAt < 1:4474413/47‑1 (MQ=16) ccATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATgt > 1:453948/1‑46 (MQ=255) atatCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATGTTAACCCCGCACTGGATg < 1:1901880/62‑1 (MQ=255) tatCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCt < 1:625380/45‑1 (MQ=255) tatCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCt < 1:1612111/45‑1 (MQ=255) tatCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGcc < 1:2106917/70‑1 (MQ=255) tatCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCgg < 1:2237207/39‑1 (MQ=16) tatCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATgtt < 1:4838486/45‑3 (MQ=255) tatCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATgtt < 1:2480542/45‑3 (MQ=255) tatCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATgtt < 1:1282650/45‑3 (MQ=255) cGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGc > 1:4708655/1‑71 (MQ=255) aTTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGt < 1:3749098/55‑1 (MQ=255) aTTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGcc < 1:3701736/71‑1 (MQ=255) ttCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCt < 1:5320668/37‑1 (MQ=255) ttCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCt < 1:208765/37‑1 (MQ=255) ttCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCt < 1:3504586/37‑1 (MQ=255) ttCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGcc < 1:4949025/70‑1 (MQ=255) cTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTg > 1:2619851/1‑47 (MQ=255) cTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGAt > 1:365107/1‑71 (MQ=255) cTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGAt > 1:974300/1‑71 (MQ=255) cTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGACGCACTGGATGTATGAAGCCTGGCTGCCGAt > 1:5258217/1‑71 (MQ=255) | GGCGTCCAGCCAGATGCTGGATCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGAT > minE/837638‑837775 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |