| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 106,043 | A→G | 16.6% | A83A (GCT→GCC) | aroP ← | aromatic amino acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 106,043 | 0 | A | G | 16.6% | 88.4 / 11.4 | 42 | A83A (GCT→GCC) | aroP | aromatic amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base A (21/14); new base G (2/5); total (23/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
AACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATG > minE/105974‑106106 | aaCTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAg > 1:725031/1‑71 (MQ=255) cTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGc > 1:4870818/1‑70 (MQ=255) cTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGc > 1:4028542/1‑70 (MQ=255) tAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCa > 1:4298674/1‑70 (MQ=255) aaCGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGt < 1:1876714/71‑1 (MQ=255) aCGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTg > 1:2065810/1‑71 (MQ=255) aCGAACATTACCCAGTAGTTCCAGCCAGAGAGGAAGCCCGCAAACGGTCCCCAGTATTTATAGGCaa < 1:4883786/67‑1 (MQ=255) aCATTACCCAGTAGTTCCAGCCAGAGAGGAAGCCCGCAAACGGTCCCCAGTATTTATAGGCAAAGTgggca < 1:1351023/71‑4 (MQ=255) aCAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCa < 1:1130490/62‑1 (MQ=255) aCAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCa < 1:5635643/62‑1 (MQ=255) cAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTaa < 1:4489955/71‑1 (MQ=255) gTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAg < 1:2718070/71‑1 (MQ=255) cccAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAg > 1:4906245/1‑59 (MQ=255) cccAGTAGTTCCAGCCAGAGAGGAAGCCCGCAAACGGTCCCCAGTATTTATAGGCa < 1:4990146/56‑1 (MQ=255) cAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGcc > 1:2739150/1‑71 (MQ=255) agtagtTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAg > 1:3061920/1‑56 (MQ=255) ttCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCa < 1:1213210/48‑1 (MQ=255) ttCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGAc < 1:3509339/71‑1 (MQ=255) ttCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGAc < 1:361639/71‑1 (MQ=255) tCCAGCCAGAGGCGAAACCGGCTAAACTGCCCCAGTATTTATAAGCAAAGTGGCTaaa > 1:2531373/1‑58 (MQ=255) ccagccagAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTaaa > 1:1183856/1‑57 (MQ=255) ccagccagAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTaaa > 1:5387778/1‑57 (MQ=255) ccagccagAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAg > 1:1694569/1‑71 (MQ=255) ccagccagAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAg > 1:994333/1‑71 (MQ=255) ccagccagAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAg > 1:4309736/1‑71 (MQ=255) cagAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGttct > 1:1899357/1‑71 (MQ=255) agagGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAg > 1:963747/1‑43 (MQ=255) gagGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTa < 1:3860395/48‑1 (MQ=255) gCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGac > 1:4109909/1‑71 (MQ=255) gCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGac > 1:2519690/1‑71 (MQ=255) aaaaCTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGaccac > 1:5197546/1‑63 (MQ=255) cTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAg < 1:5270303/71‑1 (MQ=255) tccccAGTATTTATAGGCAAAGTGGGCAAATGAACCGGATACCGGCTCCTCAACCACCATTTCGCCAAGCt < 1:5349701/70‑1 (MQ=255) tccccAGTATTTATAGGCAAAGTGGGCAAATGAACCGGATACCGGCTCCTCAACCACCATTTCGCCAAGCt < 1:1085254/70‑1 (MQ=255) gCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCt > 1:2700314/1‑71 (MQ=255) aGTATTTATAGGCAAAGTGGGCAAATGAACCGGATACCGGCTCCTCAACCACCATTTCGCCAAGCTGGCg > 1:1605763/1‑70 (MQ=255) aGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGc < 1:606703/71‑1 (MQ=255) gTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCa > 1:805067/1‑58 (MQ=255) tATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGttcttc < 1:2509002/44‑1 (MQ=255) aTTTATAGGCAAAGTGGGCAAATGAACCGGATACCGGCTCCTCAACCACCATTTCGCCAAGCTGGCGCAt > 1:1419409/1‑70 (MQ=255) tttATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTg < 1:856727/63‑1 (MQ=255) tttATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATg < 1:5494395/70‑1 (MQ=255) | AACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATG > minE/105974‑106106 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |