| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 199,598 | A→C | 6.7% | V52V (GTT→GTG) | metQ ← | DL‑methionine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 199,598 | 0 | A | C | 6.7% | 235.7 / 9.4 | 90 | V52V (GTT→GTG) | metQ | DL‑methionine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (53/31); new base C (4/2); total (57/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
TGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTG > minE/199529‑199665 | tGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCaa > 1:3594144/1‑71 (MQ=255)tGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCaa > 1:84407/1‑71 (MQ=255) gCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAAc > 1:4811834/1‑71 (MQ=255) cGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACtt < 1:2010151/69‑1 (MQ=255) gTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATATGTCTTTCGCa > 1:1984489/1‑64 (MQ=255) tGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTgcg > 1:4789289/1‑71 (MQ=255) tGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTgcg > 1:2401569/1‑71 (MQ=255) gCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTgcgc < 1:3550483/70‑1 (MQ=255) aGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCTCAACTTTCTGCGCGAc < 1:2215594/71‑1 (MQ=255) cATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACt > 1:4177215/1‑56 (MQ=255) cATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACt > 1:5668060/1‑56 (MQ=255) cATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACt > 1:5157729/1‑56 (MQ=255) cATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCt > 1:4673807/1‑71 (MQ=255) aTAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCt < 1:1337058/70‑1 (MQ=255) aGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACtt > 1:2920160/1‑54 (MQ=255) aGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACtt > 1:358721/1‑54 (MQ=255) aGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCt > 1:4783052/1‑57 (MQ=255) aGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCt > 1:2191452/1‑57 (MQ=255) gTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCa < 1:3098313/70‑1 (MQ=255) gTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCa < 1:4693274/70‑1 (MQ=255) gTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCa < 1:2439409/70‑1 (MQ=255) gTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTc > 1:1118941/1‑52 (MQ=255) gTTGAAGGTTACCAGCTCAACGTCCAGGCCATATATGTCTTTCGCAACTTTc > 1:135150/1‑52 (MQ=255) ttGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCaa < 1:3199998/67‑1 (MQ=255) gAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACttt > 1:4082564/1‑48 (MQ=255) aaGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACctgctg > 1:498431/1‑71 (MQ=255) aaGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACctgctg > 1:3125641/1‑71 (MQ=255) gTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTc < 1:237018/71‑1 (MQ=255) cctACCAGTTCAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTc > 1:44007/3‑71 (MQ=255) ttACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTgc < 1:1820407/47‑1 (MQ=255) ttACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTgc < 1:444054/47‑1 (MQ=255) aCCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTgcgc > 1:3396982/1‑47 (MQ=255) aCCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTgcgc > 1:2015192/1‑47 (MQ=255) aCCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACctg > 1:1912610/1‑62 (MQ=255) ccAGTTCAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTCTGcg > 1:4296210/1‑70 (MQ=255) cAGTTCAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTCTGcgc < 1:4101298/71‑3 (MQ=255) cAGCTCAACGTCCAGGCCATATATCACTTTCGCAACATTCTGCCCGACTTCTGCAACCTGCTGtt > 1:4735878/1‑65 (MQ=255) agttCAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTCTGCGcc < 1:1546961/68‑1 (MQ=255) cAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACt > 1:4723275/1‑44 (MQ=255) cAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACcaac > 1:3109691/1‑70 (MQ=255) cAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACcaac > 1:4920648/1‑70 (MQ=255) cAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTCTGCGCcat > 1:2335649/1‑68 (MQ=255) cAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTCTGCGCcat > 1:661592/1‑68 (MQ=255) aaCGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACcaac < 1:553301/69‑1 (MQ=255) aCGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTACTGCAACCTGCTGTTCGGCACCAACAAt < 1:2688514/71‑1 (MQ=255) tCCAGGCCATATTTGTCTTTCGCAACTTTCTTCGCGAc < 1:2473881/38‑1 (MQ=255) tCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGAc < 1:989113/38‑1 (MQ=255) tCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGAc < 1:3847435/38‑1 (MQ=255) tCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCAc < 1:3783933/71‑1 (MQ=255) tCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCAc > 1:3192328/1‑71 (MQ=255) cAGGCCTTATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGc > 1:732471/1‑71 (MQ=255) cAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTc < 1:5571799/54‑1 (MQ=255) cAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTc < 1:2996453/54‑1 (MQ=255) aGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGcc < 1:946684/71‑1 (MQ=255) ggCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAAc > 1:624659/1‑43 (MQ=255) ggCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAAc > 1:4501396/1‑43 (MQ=255) ggCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCa > 1:2362217/1‑66 (MQ=255) ccATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACg > 1:4402586/1‑66 (MQ=255) cATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCgg > 1:2293087/1‑51 (MQ=255) cATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCgg > 1:2581423/1‑51 (MQ=255) cATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACt > 1:2912895/1‑71 (MQ=255) cATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACt > 1:4797009/1‑71 (MQ=255) atatTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGAc > 1:1435920/1‑69 (MQ=255) atatTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCAGCACCAACAATCACGCCGACtt < 1:5226942/71‑1 (MQ=255) tatTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACttt < 1:4599779/71‑1 (MQ=255) atTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACttt < 1:4870568/70‑1 (MQ=255) tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAAc > 1:5120446/1‑36 (MQ=255) tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAAc > 1:548530/1‑36 (MQ=255) tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAAc > 1:2795265/1‑36 (MQ=255) tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGc > 1:3245063/1‑48 (MQ=255) tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCa > 1:3503704/1‑49 (MQ=255) tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCACCAATc > 1:1993699/1‑58 (MQ=255) tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTaa > 1:4701123/1‑71 (MQ=255) tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTa < 1:4414412/70‑1 (MQ=255) ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAAc > 1:1296223/1‑35 (MQ=255) ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACctgctg < 1:5334055/41‑1 (MQ=255) ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACctgctg < 1:2149686/41‑1 (MQ=255) ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACctgctg < 1:904095/41‑1 (MQ=255) ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACctgctg < 1:4268615/41‑1 (MQ=255) ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAt > 1:5197455/1‑71 (MQ=255) ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAt > 1:4436240/1‑71 (MQ=255) ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAt > 1:2851138/1‑71 (MQ=255) ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAt > 1:3959560/1‑71 (MQ=255) tGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAtg > 1:3724639/1‑71 (MQ=255) tGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAtg > 1:3717946/1‑71 (MQ=255) tCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGa > 1:3354070/1‑61 (MQ=255) tCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAtgtg < 1:2101692/71‑1 (MQ=255) cTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTgg > 1:2120852/1‑71 (MQ=255) tttCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATc > 1:291920/1‑52 (MQ=255) tCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTgg > 1:5155188/1‑68 (MQ=255) tCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGtt < 1:345309/70‑1 (MQ=255) cGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTg > 1:121920/1‑71 (MQ=255) cAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACcaac < 1:1999374/43‑1 (MQ=255) | TGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTG > minE/199529‑199665 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |