| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 837,734 | T→C | 19.7% | G69G (GGT→GGC) | narI → | nitrate reductase 1, gamma (cytochrome b(NR)) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 837,734 | 0 | T | C | 19.7% | 133.1 / 26.5 | 66 | G69G (GGT→GGC) | narI | nitrate reductase 1, gamma (cytochrome b(NR)) subunit |
| Reads supporting (aligned to +/- strand): ref base T (27/26); new base C (3/10); total (30/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAGTGAAACAGAAAATGGCAATGT > minE/837665‑837801 | aGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTa > 1:633968/1‑71 (MQ=255) gATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTa > 1:1127883/1‑68 (MQ=255) gAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGa > 1:4804580/1‑71 (MQ=255) cTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGAcgcc < 1:1498757/71‑1 (MQ=255) tGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGAcgccg > 1:1827984/1‑71 (MQ=255) tCGAATCTGTTCCATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATGTTAACGCCGCACTg < 1:5667574/71‑1 (MQ=255) gAATCTGTTCCATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATGTTAACGCCGCACTGGa > 1:1772263/1‑71 (MQ=255) aatcTGTTCCATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATGTTAACGCCGCACTGGAt > 1:4149972/4‑71 (MQ=255) atcTGTTCCATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATGTTAACGCCGCACt < 1:4370513/64‑1 (MQ=255) atcTGTTCCATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATGTTAACGCCGCACTGGATg < 1:3530681/69‑1 (MQ=255) atcTGTTCCATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATGTTAACGCCGCACTGGATg < 1:4836129/69‑1 (MQ=255) aCCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTa < 1:3078725/51‑1 (MQ=255) aCCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTa < 1:4547925/51‑1 (MQ=255) tGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTAt > 1:3413289/1‑71 (MQ=255) tGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTAt > 1:3813579/1‑71 (MQ=255) gTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGt < 1:1693949/68‑1 (MQ=255) ttCCATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCAt < 1:4474413/47‑1 (MQ=16) ttCCATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCAt < 1:1660383/47‑1 (MQ=16) ccATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATgt > 1:453948/1‑46 (MQ=255) atatCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATGTTAACCCCGCACTGGATg < 1:1901880/62‑1 (MQ=255) tatCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCt < 1:1612111/45‑1 (MQ=255) tatCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCt < 1:625380/45‑1 (MQ=255) tatCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGcc < 1:2106917/70‑1 (MQ=255) tatCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATgtt < 1:2480542/45‑3 (MQ=255) tatCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATgtt < 1:4838486/45‑3 (MQ=255) tatCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATgtt < 1:1282650/45‑3 (MQ=255) cGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGc > 1:4708655/1‑71 (MQ=255) aTTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGt < 1:3749098/55‑1 (MQ=255) aTTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGcc < 1:3701736/71‑1 (MQ=255) ttCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCt < 1:3504586/37‑1 (MQ=255) ttCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCt < 1:208765/37‑1 (MQ=255) ttCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCt < 1:5320668/37‑1 (MQ=255) ttCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGcc < 1:4949025/70‑1 (MQ=255) cTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTg > 1:2619851/1‑47 (MQ=255) cTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGAt > 1:974300/1‑71 (MQ=255) cTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGAt > 1:365107/1‑71 (MQ=255) cTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGACGCACTGGATGTATGAAGCCTGGCTGCCGAt > 1:5258217/1‑71 (MQ=255) gggATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTg < 1:878421/70‑1 (MQ=255) gggATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGa < 1:3201461/71‑1 (MQ=255) ggATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTAt < 1:15432/50‑1 (MQ=255) gATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAg > 1:3198810/1‑71 (MQ=255) aTTTTTGTCGGTCACTTCTTCGGTATGCTGAcgcc > 1:3579565/1‑35 (MQ=255) aTTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTg > 1:3820107/1‑41 (MQ=255) aTTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTg > 1:4697662/1‑41 (MQ=255) aTTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAGt < 1:488473/71‑1 (MQ=255) ttttGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTg < 1:2713587/39‑1 (MQ=255) ttttGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTg < 1:4893634/39‑1 (MQ=255) ttttGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTg < 1:4550046/39‑1 (MQ=255) ttttGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAg > 1:174615/1‑68 (MQ=255) ttttGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAGt > 1:3932177/1‑69 (MQ=255) ttttGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAGTg < 1:3362383/70‑1 (MQ=255) ttttGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAGTg > 1:24118/1‑70 (MQ=255) cGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGa > 1:5669370/1‑56 (MQ=255) cGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGa > 1:3958079/1‑56 (MQ=255) cGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGa > 1:1508084/1‑56 (MQ=255) ggTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTg > 1:3190265/1‑47 (MQ=255) ggTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAGTGAAACAGa > 1:2060130/1‑70 (MQ=255) ggTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAGTGAAACAGa > 1:5684658/1‑70 (MQ=255) ggTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAGTGAAACAGa > 1:3720324/1‑70 (MQ=255) tCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAGTGAAACATaaaa < 1:2201898/71‑1 (MQ=255) aCTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGAtt < 1:5530285/53‑1 (MQ=255) ttcttcGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAGTGAAACAGaaaa < 1:3202934/67‑1 (MQ=255) cttcGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAGTGAAACAGAAAATGGCa < 1:868563/70‑1 (MQ=255) cGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAg > 1:2293735/1‑50 (MQ=255) cGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAGTGAAACAGAAAATGGCaa < 1:5480955/68‑1 (MQ=255) cGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAGTGAAACAGAAAATGGCAATGt > 1:1658256/1‑71 (MQ=255) | AGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAGTGAAACAGAAAATGGCAATGT > minE/837665‑837801 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |