Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 852,453 C→T 7.3% V55V (GTG→GTA yciC ← predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE852,4530CT 7.3% 320.2 / 9.9 109V55V (GTG→GTAyciCpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (54/47);  new base T (4/4);  total (58/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 110 total aligned reads displayed.

TGTTGCTGTTCCGGTGACATATTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCTAACACCACTGTGATA  >  minE/852391‑852522
                                                              |                                                                     
tgttgCTGTTCCGGTGACATATTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGt                                                                >  1:762126/1‑70 (MQ=255)
tgttgCTGTTCCGGTGACATATTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGt                                                                <  1:5365350/70‑1 (MQ=255)
 gttgCTGTTCCGGTGACATATTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTc                                                               <  1:1548866/70‑1 (MQ=255)
    gCTGTTCCGGTGACATATTCTGAACCAGGTCTAACAACCCACTACTGCCGCTAACGGGTACGcc                                                                  <  1:713777/64‑1 (MQ=255)
    gCTGTTCCGGTGACATATTCTGAACCAGGTCGAACATCCCACTACTGCCGCTAACGGGTACGcc                                                                  <  1:2213749/64‑1 (MQ=255)
    gCTGTTCCGGTGACATATTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGTACTcc                                                                  <  1:3811013/64‑1 (MQ=255)
    gCTGTTCCGGTGACATATTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGTACGcc                                                                  <  1:273236/64‑1 (MQ=255)
     cTGTTCCGGTGACATATTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTg                                                           <  1:2823166/70‑1 (MQ=255)
     cTGTTCCGGTGACATATTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTg                                                           <  1:5592115/70‑1 (MQ=255)
       gTTCCGGTGACATATTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTg                                                           <  1:612631/68‑1 (MQ=255)
       gTTCCGGTGACATATTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGc                                                        >  1:370519/1‑71 (MQ=255)
        ttCCGGTGACATATTCTGAACCAGGTCGAACAACCCACTGCTGCCGCTAACGGGCACGCCGTCATTGAg                                                         <  1:4850546/69‑1 (MQ=255)
          ccGGTGACATATTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCAtt                                                            >  1:3646624/1‑64 (MQ=255)
          ccGGTGACATATTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTg                                                      >  1:2989942/1‑70 (MQ=255)
          ccGGTGACATATTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTg                                                      >  1:436728/1‑70 (MQ=255)
              tGACATATTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCAt                                                             >  1:5208042/1‑59 (MQ=255)
                aCATATTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCa                                                              <  1:1187161/56‑1 (MQ=255)
                aCATATTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGc                                                        >  1:5092337/1‑62 (MQ=255)
                aCATATTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGc                                               >  1:382566/1‑71 (MQ=255)
                 cATATTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGc                                               <  1:4351183/70‑1 (MQ=255)
                 cATATTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCt                                              >  1:3317050/1‑71 (MQ=255)
                   tatTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCtgt                                            >  1:5017172/1‑71 (MQ=255)
                     ttCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTc                                                               >  1:2809535/1‑50 (MQ=255)
                     ttCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCaa                                                 >  1:4819584/1‑64 (MQ=255)
                     ttCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCaa                                                 >  1:2818712/1‑64 (MQ=255)
                     ttCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCtgtg                                           >  1:1521602/1‑70 (MQ=255)
                        tGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGTACGCCGTCATTGAGCTGCGCAAGCTGTGCAt                                        >  1:5303775/1‑70 (MQ=255)
                        tGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGTACGCCGTCATTGAGCTGCGCAAGCTGTGCAt                                        >  1:493347/1‑70 (MQ=255)
                        tGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGTACGCCGTCATTGAGCTGCGCAAGCTGTGCAt                                        >  1:799163/1‑70 (MQ=255)
                        tGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGTACGCCGTCATTGAGCTGCGCAAGCTGTGCAt                                        >  1:2282734/1‑70 (MQ=255)
                        tGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAg                                                         >  1:345141/1‑53 (MQ=255)
                        tGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAg                                                         >  1:5614644/1‑53 (MQ=255)
                          aaCCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCAt                                        <  1:3747225/68‑1 (MQ=255)
                          aaCCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCAt                                        <  1:535223/68‑1 (MQ=255)
                          aaCCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCAt                                        <  1:5310957/68‑1 (MQ=255)
                          aaCCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCAt                                        <  1:1612361/68‑1 (MQ=255)
                          aaCCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCAt                                        <  1:4598481/68‑1 (MQ=255)
                          aaCCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCa                                      >  1:3964711/1‑70 (MQ=255)
                             cAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTg                                                           <  1:186943/46‑1 (MQ=255)
                             cAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTgg                                  <  1:5637260/71‑1 (MQ=255)
                             cAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTgg                                  <  1:4702492/71‑1 (MQ=255)
                              aGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTggg                                 >  1:2870781/1‑71 (MQ=255)
                               ggTGGAACAACCCACTACTGACGCTAACGGGCACGCCGt                                                                <  1:3423423/39‑1 (MQ=38)
                               ggTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAg                                                         >  1:3351792/1‑46 (MQ=255)
                               ggTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAg                                                         >  1:2091644/1‑46 (MQ=255)
                               ggTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAg                                                         >  1:2234618/1‑46 (MQ=255)
                               ggTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGt                                <  1:118444/71‑1 (MQ=255)
                                  cGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCAtt                                                            >  1:3490919/1‑40 (MQ=255)
                                  cGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCAtt                                                            >  1:432366/1‑40 (MQ=255)
                                  cGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCAt                                                             >  1:2359823/1‑39 (MQ=255)
                                  cGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCAt                                                             >  1:2626718/1‑39 (MQ=255)
                                  cGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCAt                                                             >  1:253208/1‑39 (MQ=255)
                                  cGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAg                                                         >  1:3482779/1‑43 (MQ=255)
                                  cGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAg                                                         >  1:3404492/1‑43 (MQ=255)
                                   gAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTgaga                            <  1:2283804/71‑1 (MQ=255)
                                   gAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTgag                             <  1:1118205/70‑1 (MQ=255)
                                    aacaacCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGaa                           <  1:1230569/71‑1 (MQ=255)
                                    aacaacCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGaa                           <  1:377924/71‑1 (MQ=255)
                                     acaacCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGaa                           >  1:1223714/1‑70 (MQ=255)
                                     acaacCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGaa                           >  1:3915901/1‑70 (MQ=255)
                                     acaacCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGaa                           >  1:2768570/1‑70 (MQ=255)
                                      caacCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGaaaa                         >  1:5492717/1‑71 (MQ=255)
                                          ccACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATg                     <  1:5162158/71‑1 (MQ=255)
                                           cACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGc                                               <  1:1689320/44‑1 (MQ=255)
                                           cACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGc                    <  1:1099037/71‑1 (MQ=255)
                                           cACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGc                    >  1:3881396/1‑71 (MQ=255)
                                           cACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGc                    <  1:3800349/71‑1 (MQ=255)
                                            actactGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATg                     <  1:703277/69‑1 (MQ=255)
                                            actactGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGcc                   <  1:4028870/71‑1 (MQ=255)
                                            actactGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGcc                   <  1:73699/71‑1 (MQ=255)
                                              tactGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGTGTGAGaaa                          <  1:3759069/62‑1 (MQ=255)
                                              tactGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGaaa                          <  1:3751578/62‑1 (MQ=255)
                                              tactGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGaaa                          <  1:1745343/62‑1 (MQ=255)
                                              tactGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGaaa                          <  1:4817864/62‑1 (MQ=255)
                                              tactGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGaaa                          <  1:482642/62‑1 (MQ=255)
                                              tactGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACa                       >  1:5445694/1‑65 (MQ=255)
                                              tactGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGccc                  >  1:3334111/1‑70 (MQ=255)
                                              tactGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGccc                  >  1:3692010/1‑70 (MQ=255)
                                              tactGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGccc                  >  1:1108925/1‑70 (MQ=255)
                                               actGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCt                 >  1:536953/1‑70 (MQ=255)
                                               actGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCt                 >  1:2474867/1‑70 (MQ=255)
                                               actGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCt                 >  1:3533430/1‑70 (MQ=255)
                                               actGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCt                 >  1:115454/1‑70 (MQ=255)
                                               actGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCt                 >  1:1516273/1‑70 (MQ=255)
                                                 tGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCTaa               <  1:1681030/70‑1 (MQ=255)
                                                  gCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCTAaca             >  1:3882112/1‑71 (MQ=255)
                                                     gCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCTaa               <  1:3548208/66‑1 (MQ=255)
                                                     gCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCTaa               <  1:5436483/66‑1 (MQ=255)
                                                      cTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATc                                       <  1:1263637/41‑1 (MQ=255)
                                                         aCGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCTAACACCACt        <  1:4288069/69‑1 (MQ=255)
                                                          cGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCTAACACCACtgt      >  1:2423880/1‑70 (MQ=255)
                                                           gggCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCTAACACCACTGTGa    >  1:4242536/1‑71 (MQ=255)
                                                            ggCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATg                     >  1:1134635/1‑53 (MQ=255)
                                                            ggCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATg                     >  1:3110146/1‑53 (MQ=255)
                                                             gCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCTAACACCACTGTGAt   <  1:5170632/70‑1 (MQ=255)
                                                             gCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCTAACACCACTGTGATa  <  1:296880/71‑1 (MQ=255)
                                                             gCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCTAACACCACTGTGATa  <  1:2911368/71‑1 (MQ=255)
                                                             gCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCTAACACCACTGTGATa  <  1:4793579/71‑1 (MQ=255)
                                                             gCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCTAACACCACTGTGATa  <  1:1839179/71‑1 (MQ=255)
                                                             ccACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCTAACACCACTGTGATa  <  1:2013055/70‑1 (MQ=255)
                                                              |                                                                     
TGTTGCTGTTCCGGTGACATATTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCTAACACCACTGTGATA  >  minE/852391‑852522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: