| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 854,861 | C→T | 19.7% | A43A (GCG→GCA) | yciG ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 854,861 | 0 | C | T | 19.7% | 143.7 / 27.9 | 71 | A43A (GCG→GCA) | yciG | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (28/29); new base T (6/8); total (34/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
TTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCT > minE/854792‑854927 | ttAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCg > 1:345959/1‑71 (MQ=255) aataatTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCt > 1:3677709/1‑71 (MQ=255) atTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCa < 1:4757041/70‑1 (MQ=255) atTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCa < 1:4980622/70‑1 (MQ=255) atTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAg < 1:257891/71‑1 (MQ=255) atTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCACCTTTTTTACCCGCTTCa < 1:2275423/70‑1 (MQ=255) tGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCa < 1:3061500/62‑1 (MQ=255) aaTCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTg > 1:5177215/1‑71 (MQ=255) aaTCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTg < 1:2085009/71‑1 (MQ=255) aaTCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTg > 1:1928582/1‑71 (MQ=255) tCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCg < 1:4809467/71‑1 (MQ=255) cAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCa < 1:4094745/57‑1 (MQ=255) cAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCa < 1:2758171/57‑1 (MQ=255) cAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCa < 1:4607183/57‑1 (MQ=255) cAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCa < 1:4616181/57‑1 (MQ=255) ggATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGa > 1:204844/1‑57 (MQ=255) gATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTg < 1:5578218/65‑1 (MQ=255) tttCCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCAt < 1:706094/71‑1 (MQ=255) tGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCAttt > 1:1673781/1‑71 (MQ=255) ccTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCg < 1:1303607/61‑1 (MQ=255) tGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCa < 1:1515083/46‑1 (MQ=255) tGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCa < 1:5152521/46‑1 (MQ=255) tGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCgg > 1:1222567/1‑60 (MQ=255) tGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTa > 1:1199558/1‑70 (MQ=255) aTTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTaa < 1:4841389/69‑1 (MQ=255) tattaCCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCa > 1:785152/1‑41 (MQ=255) tattaCCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTa > 1:4708965/1‑71 (MQ=255) ttaCCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTAc < 1:4865645/70‑1 (MQ=255) ccaccGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTAccgc < 1:1450435/70‑1 (MQ=255) accGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACcgccgc < 1:4981776/71‑1 (MQ=255) ccGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGa > 1:5148255/1‑35 (MQ=255) ccGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGa > 1:3582631/1‑35 (MQ=255) ccGCTTTGTTGACCGCCTTTTTTACACGCTTCAGa > 1:3891033/1‑35 (MQ=255) cGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTa < 1:2394448/71‑1 (MQ=255) cTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATg > 1:1814136/1‑71 (MQ=255) tgttgACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCt > 1:599170/1‑70 (MQ=255) ttaccaccTTTTTTACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAATTAccccc > 1:2537712/3‑59 (MQ=255) taccaccTTTTTTACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAATTACCCCCGCTGTGCTGTcca > 1:5201179/2‑70 (MQ=255) gACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTa < 1:262660/62‑1 (MQ=255) accaccTTTTTTACCTGCTTCAGATGCGCGCTGCGGGTCAtt > 1:4085450/1‑42 (MQ=25) accaccTTTTTTACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAATTACCCCCGCTGTGCTgtc < 1:2902977/68‑3 (MQ=255) accaccTTTTTTACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAATTACCCCCGCTGTGCTGTccac > 1:2807254/1‑69 (MQ=255) accaccTTTTTTACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAATTACCCCCGCTGTGCTGTcca < 1:1115050/70‑2 (MQ=255) accaccTTTTTTACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAATTACCCCCGCTGTGCTGTcca < 1:2408153/70‑2 (MQ=255) accaccTTTTTTACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAATTACCCCCGCTGTGCTGTcca < 1:2414555/70‑2 (MQ=255) aCCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGAcctc > 1:574382/1‑69 (MQ=255) ccGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGAt > 1:2708169/1‑37 (MQ=255) gCCTTTTTTACCCGCTTCAGATGCGCGTTGCGTATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCtt < 1:3742697/71‑1 (MQ=255) gCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCtt < 1:3456577/71‑1 (MQ=255) gCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCtt < 1:49739/71‑1 (MQ=255) ccTTTTTTACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAATTACCCCCGCTGTGCTGTCCACCttt > 1:504233/1‑71 (MQ=255) cTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCttt < 1:1081837/70‑1 (MQ=255) ttttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGc < 1:3452642/65‑1 (MQ=255) ttttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTAc > 1:226188/1‑71 (MQ=255) tttttACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAAt < 1:3374548/43‑1 (MQ=255) ttttACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAATTa > 1:223896/1‑44 (MQ=255) ttttACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAATTACCCCCGCTGTGCTGTCCACCttttt < 1:1200742/69‑3 (MQ=255) ttttACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAATTACCCCCGCTGTGCTGTCCACCttttt < 1:2860583/69‑3 (MQ=255) ttttACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAATTACCCCCGCTGTGCTGTCCACCttttt < 1:3709759/69‑3 (MQ=255) ttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTaaaa > 1:2976150/1‑41 (MQ=255) ttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTa > 1:3626140/1‑38 (MQ=255) ttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTa > 1:2882276/1‑38 (MQ=255) ttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTa > 1:4983566/1‑44 (MQ=255) ttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTa > 1:4627231/1‑44 (MQ=255) ttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTa > 1:4340307/1‑44 (MQ=255) ttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTa > 1:3777310/1‑44 (MQ=255) ttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTAc > 1:4608047/1‑45 (MQ=255) ttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTAc > 1:1949034/1‑45 (MQ=255) ttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACgg > 1:1301709/1‑71 (MQ=255) tACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCtt < 1:1418166/63‑1 (MQ=255) aCCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCt < 1:2288744/70‑1 (MQ=255) cGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTa > 1:276554/1‑46 (MQ=255) | TTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCT > minE/854792‑854927 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |