| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 910,896 | A→T | 23.6% | T46S (ACC→TCC) ‡ | ycjV → | predicted sugar transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 910,896 | 0 | A | T | 23.6% | 119.7 / 28.4 | 55 | T46S (ACC→TCC) ‡ | ycjV | predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (24/18); new base T (5/8); total (29/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01 | |||||||||||
CAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATG > minE/910829‑910964 | cAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTTAGTCGaccacc < 1:1217734/70‑1 (MQ=255)cAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGaccac < 1:3993161/69‑1 (MQ=255)cAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGaccac < 1:144637/69‑1 (MQ=255)cAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCAccc > 1:824059/1‑71 (MQ=255) aaCCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCt > 1:3805422/1‑71 (MQ=255) ccTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTgc > 1:1101231/1‑71 (MQ=255) gCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCt > 1:3518709/1‑59 (MQ=255) aTAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCggg > 1:2677742/1‑71 (MQ=255) aTAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCggg < 1:4313803/71‑1 (MQ=255) tAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGAGGTAAGTCGACCACCCTGCGCATGATTGCCGGGc > 1:2904991/1‑71 (MQ=255) aaaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGcc < 1:1423039/66‑1 (MQ=255) aaaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCgg > 1:3532415/1‑68 (MQ=255) aaaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCgg > 1:3030204/1‑68 (MQ=255) aaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCtt < 1:1776606/71‑1 (MQ=255) aaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCtt < 1:397932/71‑1 (MQ=255) aaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCtt < 1:5631049/71‑1 (MQ=255) aaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCt > 1:4948168/1‑70 (MQ=255) agagTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGAtt < 1:4549639/61‑1 (MQ=255) agagTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGAtt < 1:4067487/61‑1 (MQ=255) agagTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGAtt < 1:2217567/61‑1 (MQ=255) aTCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGgaga < 1:1087574/70‑1 (MQ=255) tCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATc < 1:5374022/71‑1 (MQ=255) gtgtTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAg < 1:4314524/71‑1 (MQ=255) gtgtTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAg > 1:1155616/1‑71 (MQ=255) gtgtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCac > 1:888846/1‑70 (MQ=9) gtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCac > 1:3636701/1‑68 (MQ=2) tttGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATg < 1:2215401/45‑1 (MQ=255) tttGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTgag < 1:4453641/60‑1 (MQ=255) tttGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTgag < 1:3017729/60‑1 (MQ=255) tttGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATc < 1:394054/66‑1 (MQ=2) ttGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcgg > 1:404954/1‑70 (MQ=255) tGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcggcg > 1:3345148/1‑71 (MQ=255) ccGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCt > 1:216732/1‑71 (MQ=2) cGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTg < 1:127381/71‑1 (MQ=9) cGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTg < 1:1656270/71‑1 (MQ=9) cGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTg < 1:120551/71‑1 (MQ=9) tctggCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGtt < 1:281672/68‑1 (MQ=9) tCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGtt > 1:4159067/1‑71 (MQ=255) tCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGtt > 1:5202624/1‑71 (MQ=255) tggCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGttca > 1:389365/2‑69 (MQ=16) gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGt > 1:1951081/1‑68 (MQ=255) gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTg > 1:811305/1‑70 (MQ=255) gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTg < 1:5055415/70‑1 (MQ=255) gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGa > 1:1129392/1‑71 (MQ=255) gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGa > 1:2278525/1‑71 (MQ=255) gCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGttca < 1:1958908/69‑3 (MQ=16) cTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCg > 1:19106/1‑71 (MQ=255) tGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTga < 1:3188505/41‑1 (MQ=16) cGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCggt < 1:245155/70‑3 (MQ=255) ggTAAGTCGACCACCCTGCGCATGATTGCCGGGCtt < 1:4409270/36‑1 (MQ=255) tAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCa < 1:3368712/71‑1 (MQ=255) aaGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCa > 1:747362/1‑70 (MQ=255) aGTCGACCACCCTGCGCATGATTGCCGGGCTTgag > 1:219384/1‑35 (MQ=255) aGTCGACCACCCTGCGCATGATTGCCGGGCTTgag < 1:3147373/35‑1 (MQ=255) aGTCGACCACCCTGCGCATGATTGCCGGGCTTgag > 1:3091523/1‑35 (MQ=255) aGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCaaa > 1:3327204/1‑71 (MQ=255) gACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaa > 1:4028421/1‑71 (MQ=255) gACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaa > 1:1960426/1‑71 (MQ=255) accaccCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTg < 1:315964/54‑1 (MQ=255) caccCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaatg > 1:2835923/1‑70 (MQ=255) | CAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATG > minE/910829‑910964 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |