| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 910,923 | G→A | 28.8% | E55K (GAG→AAG) ‡ | ycjV → | predicted sugar transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 910,923 | 0 | G | A | 28.8% | 115.1 / 43.4 | 66 | E55K (GAG→AAG) ‡ | ycjV | predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (36/11); new base A (6/13); total (42/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.38e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAA > minE/910857‑910990 | aTCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGgaga < 1:1087574/70‑1 (MQ=255) tCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATc < 1:5374022/71‑1 (MQ=255) gtgtTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAg > 1:1155616/1‑71 (MQ=255) gtgtTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAg < 1:4314524/71‑1 (MQ=255) gtgtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCac > 1:888846/1‑70 (MQ=9) gtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCac > 1:3636701/1‑68 (MQ=2) tttGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATc < 1:394054/66‑1 (MQ=2) ttGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcgg > 1:404954/1‑70 (MQ=255) tGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcggcg > 1:3345148/1‑71 (MQ=255) ccGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCt > 1:216732/1‑71 (MQ=2) cGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTg < 1:1656270/71‑1 (MQ=9) cGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTg < 1:127381/71‑1 (MQ=9) cGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTg < 1:120551/71‑1 (MQ=9) tctggCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGtt < 1:281672/68‑1 (MQ=9) tCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGtt > 1:5202624/1‑71 (MQ=255) tCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGtt > 1:4159067/1‑71 (MQ=255) tggCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGttca > 1:389365/2‑69 (MQ=16) gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGt > 1:1951081/1‑68 (MQ=255) gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTg > 1:811305/1‑70 (MQ=255) gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTg < 1:5055415/70‑1 (MQ=255) gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGa > 1:1129392/1‑71 (MQ=255) gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGa > 1:2278525/1‑71 (MQ=255) gCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGttca < 1:1958908/69‑3 (MQ=16) cTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCg > 1:19106/1‑71 (MQ=255) cGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCggt < 1:245155/70‑3 (MQ=255) tAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCa < 1:3368712/71‑1 (MQ=255) aaGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCa > 1:747362/1‑70 (MQ=255) aGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCaaa > 1:3327204/1‑71 (MQ=255) gACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaa > 1:4028421/1‑71 (MQ=255) gACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaa > 1:1960426/1‑71 (MQ=255) accaccCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTg < 1:315964/54‑1 (MQ=255) caccCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaatg > 1:2835923/1‑70 (MQ=255) tacTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACggatgaat > 1:4872102/3‑71 (MQ=255) acTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACggatgaat < 1:617487/69‑1 (MQ=255) cTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGt > 1:2493386/1‑46 (MQ=255) cTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGt > 1:5182558/1‑46 (MQ=255) cTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGAt > 1:3697615/1‑50 (MQ=255) cTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACggatgaat < 1:4600486/69‑1 (MQ=255) cTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGatgaatga < 1:357269/71‑1 (MQ=255) tGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGa > 1:5325610/1‑52 (MQ=255) tGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGa > 1:4735850/1‑52 (MQ=255) tGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGa > 1:4463217/1‑52 (MQ=255) tGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCaaa > 1:1088077/1‑59 (MQ=255) cgcATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGt > 1:3574296/1‑71 (MQ=255) cgcATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGatgaatga < 1:2731504/68‑1 (MQ=255) gcATGATTGCCGGGCTTGAGGAGATCAGCGGAGGCGATCTGTTGATCGACGGCAAACgaatgaat < 1:233013/65‑1 (MQ=255) aTGATTGCCGGGCTTGAGACGATCACCAGCGGCGACATGtt < 1:1951221/41‑1 (MQ=12) gATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAg > 1:2038626/1‑71 (MQ=255) gATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAg > 1:4874606/1‑71 (MQ=255) gATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTTTTCATCggt < 1:2110753/46‑3 (MQ=255) aTTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGc > 1:5464613/1‑71 (MQ=255) aTTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGc > 1:2111343/1‑71 (MQ=255) ttGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGc > 1:3299400/1‑70 (MQ=255) ttGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGc > 1:5085129/1‑70 (MQ=255) tGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaa > 1:2537131/1‑53 (MQ=255) tGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGcc > 1:3397989/1‑70 (MQ=255) tGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGcc > 1:5360278/1‑70 (MQ=255) tGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGcc > 1:3391508/1‑70 (MQ=255) gCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaatg < 1:2574722/54‑1 (MQ=255) ggCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaatgaat > 1:4148776/1‑53 (MQ=255) ggCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaatgaat > 1:3226819/1‑53 (MQ=255) ggCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCa < 1:5373925/66‑1 (MQ=255) ggCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCa < 1:3291440/66‑1 (MQ=255) ggCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCa > 1:1218710/1‑71 (MQ=255) ttGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACg < 1:446288/43‑1 (MQ=255) ttGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACg < 1:2853669/43‑1 (MQ=255) tGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGc > 1:4399760/1‑70 (MQ=255) aggagATCAGCGGCGGCGATCTGTTGATCGACTGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCaa < 1:1740306/70‑1 (MQ=255) aggagATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCa > 1:2190602/1‑56 (MQ=255) aggagATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCa > 1:2164008/1‑56 (MQ=255) gagaTCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTc < 1:4989657/52‑1 (MQ=255) gagaTCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTc < 1:2626302/52‑1 (MQ=255) | ATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAA > minE/910857‑910990 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |