| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 910,946 | G→C | 10.8% | L62F (TTG→TTC) | ycjV → | predicted sugar transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 910,946 | 0 | G | C | 10.8% | 200.5 / 12.3 | 74 | L62F (TTG→TTC) | ycjV | predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (38/28); new base C (2/6); total (40/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01 | |||||||||||
GGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAACTACG > minE/910877‑911016 | gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTg > 1:811305/1‑70 (MQ=255)gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTg < 1:5055415/70‑1 (MQ=255)gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGa > 1:1129392/1‑71 (MQ=255)gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGa > 1:2278525/1‑71 (MQ=255) cTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACC‑TGTTCATCg > 1:19106/1‑71 (MQ=255) cGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACC‑TGTTCATCggt < 1:245155/70‑3 (MQ=255) tAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCa < 1:3368712/71‑1 (MQ=255) aaGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCa > 1:747362/1‑70 (MQ=255) aGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCaaa > 1:3327204/1‑71 (MQ=255) gACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACgaa > 1:4028421/1‑71 (MQ=255) gACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACgaa > 1:1960426/1‑71 (MQ=255) accaccCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTg < 1:315964/54‑1 (MQ=255) caccCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACgaatg > 1:2835923/1‑70 (MQ=255) tacTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACC‑TGTTCATCGGTGAGAAACggatgaat > 1:4872102/3‑71 (MQ=255) acTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACC‑TGTTCATCGGTGAGAAACggatgaat < 1:617487/69‑1 (MQ=255) cTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGAt > 1:3697615/1‑50 (MQ=255) cTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACC‑TGTTCATCGGTGAGAAACggatgaat < 1:4600486/69‑1 (MQ=255) cTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACC‑TGTTCATCGGTGAGAAACGGatgaatga < 1:357269/71‑1 (MQ=255) tGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGa > 1:4463217/1‑52 (MQ=255) tGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGa > 1:4735850/1‑52 (MQ=255) tGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGa > 1:5325610/1‑52 (MQ=255) tGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCaaa > 1:1088077/1‑59 (MQ=255) cgcATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACGAATGAATGACGt > 1:3574296/1‑71 (MQ=255) cgcATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACC‑TGTTCATCGGTGAGAAACGGatgaatga < 1:2731504/68‑1 (MQ=255) gcATGATTGCCGGGCTTGAGGAGATCAGCGGAGGCGATC‑TGTTGATCGACGGCAAACgaatgaat < 1:233013/65‑1 (MQ=255) gATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCAg > 1:2038626/1‑71 (MQ=255) gATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCAg > 1:4874606/1‑71 (MQ=255) gATTGCCGGGCTTGAGACGATCACCAGCGGCGACC‑TTTTCATCggt < 1:2110753/46‑3 (MQ=255) aTTGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCAGc > 1:5464613/1‑71 (MQ=255) aTTGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCAGc > 1:2111343/1‑71 (MQ=255) ttGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCAGc > 1:3299400/1‑70 (MQ=255) ttGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCAGc > 1:5085129/1‑70 (MQ=255) tGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACgaa > 1:2537131/1‑53 (MQ=255) tGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCAGcc > 1:5360278/1‑70 (MQ=255) tGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCAGcc > 1:3397989/1‑70 (MQ=255) tGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCAGcc > 1:3391508/1‑70 (MQ=255) gCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACgaatg < 1:2574722/54‑1 (MQ=255) ggCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACgaatgaat > 1:3226819/1‑53 (MQ=255) ggCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACgaatgaat > 1:4148776/1‑53 (MQ=255) ggCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCa < 1:3291440/66‑1 (MQ=255) ggCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCa < 1:5373925/66‑1 (MQ=255) ggCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCa > 1:1218710/1‑71 (MQ=255) ttGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACg < 1:2853669/43‑1 (MQ=255) ttGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACg < 1:446288/43‑1 (MQ=255) tGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGc > 1:4399760/1‑70 (MQ=255) aggagATCAGCGGCGGCGATC‑TGTTGATCGACTGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCaa < 1:1740306/70‑1 (MQ=255) aggagATCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCa > 1:2164008/1‑56 (MQ=255) aggagATCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCa > 1:2190602/1‑56 (MQ=255) gagaTCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTc < 1:2626302/52‑1 (MQ=255) gagaTCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTc < 1:4989657/52‑1 (MQ=255) aTCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAg < 1:5521638/58‑1 (MQ=255) aGCGGCGGCGATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGa < 1:3411660/70‑1 (MQ=255) ggcggcgATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGa < 1:2257622/67‑1 (MQ=255) ggcggcgATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGa < 1:3307363/67‑1 (MQ=255) ggcgATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACg > 1:4398509/1‑53 (MQ=255) ggcgATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGtt > 1:4005116/1‑71 (MQ=255) gcgATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCaa < 1:3354471/55‑1 (MQ=255) gcgATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCaa < 1:1407766/55‑1 (MQ=255) gcgATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCaa < 1:3359202/55‑1 (MQ=255) gcgATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTc < 1:158861/71‑1 (MQ=255) ccgATCTTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAg > 1:2699680/2‑49 (MQ=255) aTC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCa < 1:2900474/51‑1 (MQ=255) tGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGc < 1:5012083/47‑1 (MQ=255) tGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGc < 1:802163/55‑1 (MQ=255) tGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGc < 1:1088744/55‑1 (MQ=255) tGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGc < 1:4241606/55‑1 (MQ=255) tGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGc < 1:2534246/55‑1 (MQ=255) tGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGa > 1:2297004/1‑57 (MQ=255) gTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAAc > 1:492713/1‑70 (MQ=255) gTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAAc > 1:4642395/1‑70 (MQ=255) gTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAAc > 1:890858/1‑70 (MQ=255) gTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTCTTCCAGAAc > 1:1172287/1‑70 (MQ=255) ttGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGAtt < 1:793852/57‑2 (MQ=255) ttGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAg < 1:566850/66‑1 (MQ=255) ttGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAAc < 1:4953420/69‑1 (MQ=255) ttGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAAc < 1:1640297/69‑1 (MQ=255) ttGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAACTa > 1:2992337/1‑71 (MQ=255) gATCGACGGCAAACGAATGAATGACGTTCCCGCCa > 1:648233/1‑35 (MQ=255) gATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAata > 1:55859/1‑47 (MQ=255) gATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAACTAcg > 1:947769/1‑71 (MQ=255) | GGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATC‑TGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAACTACG > minE/910877‑911016 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |