| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 965,844 | A→T | 83.8% | V17D (GTT→GAT) | ydeM ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 965,844 | 0 | A | T | 83.8% | 213.1 / 30.0 | 86 | V17D (GTT→GAT) | ydeM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (8/6); new base T (38/34); total (46/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
AGAGTGCTGTCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGG > minE/965778‑965913 | agagTGCTGTCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAtcac < 1:91415/70‑1 (MQ=255) agTGCTGTCATCCATCCGTTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACAtt < 1:387267/71‑1 (MQ=255) agTGCTGTCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATcaca > 1:5499357/1‑69 (MQ=255) agTGCTGTCATCCATCCATTTTTAATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACAtt > 1:4984403/1‑71 (MQ=255) tGCTGTCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTT‑AAGGTAAAAACAGTAATCACATTTga < 1:4796408/71‑1 (MQ=255) cTGTCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTgag < 1:4952136/71‑1 (MQ=255) cTGTCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTga < 1:4921839/70‑1 (MQ=255) tCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTga < 1:515332/67‑1 (MQ=255) tCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTga < 1:2309148/67‑1 (MQ=255) tCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTga < 1:2300436/67‑1 (MQ=255) tCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGAt < 1:5388274/70‑1 (MQ=255) tCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGAt < 1:3634251/70‑1 (MQ=255) atccatccATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTa > 1:3100986/1‑70 (MQ=255) atccatccATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTa < 1:4842690/70‑1 (MQ=255) ccatccatTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACAt > 1:5110919/1‑71 (MQ=255) atccatTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTa < 1:3492584/66‑1 (MQ=255) ccatTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTa < 1:5528483/64‑1 (MQ=255) catTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACAttt > 1:3245571/1‑56 (MQ=255) catTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGaa < 1:650236/70‑1 (MQ=255) catTTTTCATGAGTAAACTGCGACTCATTTTCAAGGTAAAAACAGTAATCACAttt > 1:3529734/1‑56 (MQ=39) tttttCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACAttt < 1:4198136/54‑1 (MQ=255) tttttCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACAt < 1:2396778/52‑1 (MQ=255) tttttCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTg < 1:3870497/55‑1 (MQ=255) tttCATGAGTAAACTGCGACTTTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTg > 1:4356211/1‑71 (MQ=39) tttCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTaaa > 1:5009941/1‑45 (MQ=255) tttCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGAt > 1:2223786/1‑57 (MQ=255) tttCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGaaaa > 1:3439668/1‑68 (MQ=255) tttCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAAc > 1:5171920/1‑69 (MQ=255) tttCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTAAATTGAAAACTg > 1:3986780/1‑71 (MQ=255) tttCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTgaga > 1:1498619/1‑56 (MQ=255) tttCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTgag > 1:1013720/1‑55 (MQ=255) tttCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTgag > 1:2335833/1‑55 (MQ=255) tttCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTAc > 1:1716406/1‑60 (MQ=255) tCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTg > 1:3080889/1‑69 (MQ=255) tCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTgg < 1:4028570/70‑1 (MQ=255) cATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTgg < 1:2707478/69‑1 (MQ=255) cATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTg > 1:1963503/1‑50 (MQ=255) cATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTg > 1:1199636/1‑50 (MQ=255) cATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTg > 1:86428/1‑50 (MQ=255) aTGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACAttt < 1:2670012/48‑1 (MQ=255) aTGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAgg < 1:5072238/71‑1 (MQ=255) tGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAggg < 1:1482876/71‑1 (MQ=255) gTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACAt > 1:646930/1‑54 (MQ=255) gTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCtt < 1:246340/71‑1 (MQ=255) tAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTa < 1:24997/50‑1 (MQ=255) cTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTga < 1:1973096/41‑1 (MQ=255) cTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTga < 1:4014642/41‑1 (MQ=255) tGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTg < 1:5653779/50‑1 (MQ=255) tGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTg < 1:452207/50‑1 (MQ=255) tGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTg > 1:931420/1‑70 (MQ=255) cGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTTAGATTACATTGaa > 1:3381366/1‑50 (MQ=25) cGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGaa > 1:3538365/1‑50 (MQ=255) cGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGaa > 1:4997373/1‑50 (MQ=255) cGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACt < 1:721373/54‑1 (MQ=255) cGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACt < 1:2171601/54‑1 (MQ=255) ctctTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTa > 1:4545080/1‑40 (MQ=255) ctctTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGaaaa > 1:4694771/1‑49 (MQ=255) ctctTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGaaaa > 1:3883818/1‑49 (MQ=255) ctctTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGt > 1:4282867/1‑71 (MQ=255) ctctTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGt > 1:3226067/1‑71 (MQ=255) tctTTTTCAATGTAAAAACAGTAATCACATTTGAGATTACATTGaa < 1:881176/46‑1 (MQ=37) tctTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGt < 1:3089325/65‑1 (MQ=255) tttttCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGaaaa > 1:4234491/1‑46 (MQ=255) tttttCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGaaaa > 1:2858938/1‑46 (MQ=255) tttttCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGc < 1:1655884/60‑1 (MQ=255) tttCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGaa > 1:3272981/1‑42 (MQ=255) ttCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCAt < 1:4736945/69‑1 (MQ=255) cAAGGTAAAAACAGTAATCACATTTGAGATTACATTGaaa > 1:4258970/1‑40 (MQ=255) cAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCg > 1:685484/1‑69 (MQ=255) aaGTTAATAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCt < 1:4332518/70‑1 (MQ=255) gTAAAAACAGTAATCACATTTGAGATTACATTGAAAACt > 1:551372/1‑39 (MQ=255) gTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCa > 1:2550161/1‑62 (MQ=255) tAAAAACAGTAATCACATTTGAGATTACATTGaaa < 1:1300204/35‑1 (MQ=255) tAAAAACAGTAATCACATTTGAGATTACATTGaaa < 1:125606/35‑1 (MQ=255) aaaaaCAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTgg > 1:604799/1‑48 (MQ=255) aaaaCAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCAt < 1:1006246/60‑1 (MQ=255) aaaaCAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCAt < 1:1417644/60‑1 (MQ=255) aCAGTAATCACATTTGAGATTACATTGAAAACTGGa > 1:5227133/1‑36 (MQ=255) aCAGTAATCACATTTGAGATTACATTGAAAACTGGAg > 1:1569186/1‑37 (MQ=255) aCAGTAATCACATTTGAGATTACATTGAAAACTGGAg > 1:3596215/1‑37 (MQ=255) aCAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTAtct > 1:4244173/1‑65 (MQ=255) aCAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTAtct > 1:5403224/1‑65 (MQ=255) aCAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTAtct > 1:5484103/1‑65 (MQ=255) aCAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTAtct > 1:1366776/1‑65 (MQ=255) cAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAAc > 1:1309819/1‑50 (MQ=255) aGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAAt < 1:3497615/71‑1 (MQ=255) aGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAAt < 1:5089946/71‑1 (MQ=255) tAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAAt > 1:4935096/1‑69 (MQ=255) aaCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAgg > 1:5281260/1‑71 (MQ=255) | AGAGTGCTGTCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGG > minE/965778‑965913 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |