| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 974,990 | A→T | 70.7% | intergenic (+742/+250) | lsrB → / ← ydeH | AI2 transporter/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 974,990 | 0 | A | T | 70.7% | 111.1 / 59.7 | 75 | intergenic (+742/+250) | lsrB/ydeH | AI2 transporter/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (10/12); new base T (24/29); total (34/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 | |||||||||||
ACTGGCAAATTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCTAAGTAAGTTGCCA > minE/974922‑975053 | aCTGGCAAATTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCt > 1:454050/1‑71 (MQ=255)aCTGGCAAATTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCt > 1:3118925/1‑71 (MQ=255)aCTGGCAAATTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCt > 1:1597239/1‑71 (MQ=255)aCTGGCAAATTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCt < 1:3168915/71‑1 (MQ=255)aCTGGCAAATTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATAc < 1:151066/70‑1 (MQ=255) ggCAAATTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCg > 1:568763/1‑71 (MQ=255) gCAAATTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAAttcttc < 1:5250062/69‑1 (MQ=255) gCAAATTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGc > 1:3808385/1‑71 (MQ=255) cAAATTCTGACACCTGCACGACATGCTTCTTCATCTTTAGCCGCTTTGACAATAATGATAAATACt < 1:2651586/66‑1 (MQ=255) cAAATTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACt < 1:4625247/66‑1 (MQ=255) aaaTTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGccc > 1:1569809/1‑71 (MQ=255) aaaTTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGccc > 1:1222844/1‑71 (MQ=255) aaaTTCTGACACCTGCACGACATGCTTCTTCATAATTAGCCGCTTTGACAATAATGATAAATACTTCGccc > 1:720883/1‑71 (MQ=255) aaTTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGcccc < 1:4624775/71‑1 (MQ=255) aTTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGccccc > 1:5488270/1‑71 (MQ=255) cTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCg > 1:3686445/1‑69 (MQ=255) cTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGt < 1:3146865/70‑1 (MQ=255) acacCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGc < 1:318360/70‑1 (MQ=255) gCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATaaa > 1:3162256/1‑70 (MQ=255) gCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATa > 1:1129987/1‑68 (MQ=255) gCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAAc > 1:5487342/1‑71 (MQ=255) cGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGt < 1:4149173/71‑1 (MQ=255) tGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGc < 1:2858456/56‑1 (MQ=255) tGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGt < 1:3887291/71‑1 (MQ=255) tGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGt < 1:5277042/71‑1 (MQ=255) tGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGt < 1:2073717/71‑1 (MQ=255) gCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGt > 1:4779493/1‑70 (MQ=255) gCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGt > 1:4605926/1‑70 (MQ=255) cttcttCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGc > 1:4189037/1‑45 (MQ=255) cttcttCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAAc > 1:5428693/1‑61 (MQ=255) cttcttCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTAGTaa > 1:3637422/1‑71 (MQ=255) tcatcaTTAGCCGCTTTGACAATAATGATAAATTCTTCg > 1:2670062/1‑39 (MQ=255) catcatTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCAc < 1:3052720/69‑1 (MQ=255) catcatTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCAc < 1:3758576/69‑1 (MQ=255) atcatTAGCCGCTTTGACAATAATGATAAAttcttc > 1:1277513/1‑36 (MQ=255) tcatTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATa < 1:1442424/50‑1 (MQ=255) tcatTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAAt < 1:2058439/64‑1 (MQ=255) catTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTc > 1:485284/1‑71 (MQ=255) catTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTc > 1:5678461/1‑71 (MQ=255) catTAGCAGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTc > 1:4622707/1‑71 (MQ=255) atTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTc > 1:1568312/1‑70 (MQ=255) gCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAAt > 1:2234746/1‑58 (MQ=255) gCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAAc < 1:4493710/70‑1 (MQ=255) gCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACt > 1:5143582/1‑71 (MQ=255) gCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACt < 1:5494066/71‑1 (MQ=255) gCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAAc > 1:936965/1‑44 (MQ=255) gCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAAt < 1:1672963/55‑1 (MQ=255) cTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATATACCGtt < 1:5868/47‑1 (MQ=25) cTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGtt < 1:1411235/47‑1 (MQ=255) ttGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCa > 1:1482512/1‑54 (MQ=255) ttGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCa < 1:673418/62‑1 (MQ=255) ttGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCa < 1:59323/62‑1 (MQ=255) ttGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCt > 1:2465626/1‑69 (MQ=255) tGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCtaag > 1:2195049/1‑71 (MQ=255) tGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCtaa < 1:2199451/70‑1 (MQ=255) tGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCtaa < 1:1701111/70‑1 (MQ=255) tGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCtaa < 1:3104732/70‑1 (MQ=255) tGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCta < 1:1848892/69‑1 (MQ=255) gACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTc > 1:5165567/1‑45 (MQ=255) aCAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCtaagt < 1:2545339/70‑1 (MQ=255) aCAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCtaagt < 1:4878970/70‑1 (MQ=255) cAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCtaagta < 1:2822703/70‑1 (MQ=255) cAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACt < 1:2154759/61‑1 (MQ=255) cAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACt < 1:4045593/61‑1 (MQ=255) cAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACt < 1:3427124/61‑1 (MQ=255) aataatGATAAATTCTTCGCCCCCGTAGCGATAAACCg > 1:3568427/1‑38 (MQ=255) ataatGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGt < 1:3645971/43‑1 (MQ=255) ataatGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTaa < 1:5562736/45‑1 (MQ=255) ataatGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCTTCCAACTGGCtaagtaag < 1:1123286/70‑1 (MQ=255) atGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCTAAGTAAGTTGc < 1:2569442/71‑1 (MQ=255) tGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGt < 1:3190935/39‑1 (MQ=255) tGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAAt < 1:2826843/42‑1 (MQ=255) tGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCTAAGTAAGTTGcc < 1:2755606/71‑1 (MQ=255) aTAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCTAAGTAAGTTGCCa < 1:1998514/70‑1 (MQ=255) tAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCtaag > 1:5060459/1‑59 (MQ=255) tAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCtaag > 1:4617259/1‑59 (MQ=255) tAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCtaag > 1:3051362/1‑59 (MQ=255) | ACTGGCAAATTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCTAAGTAAGTTGCCA > minE/974922‑975053 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |