Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 980,251 A→G 13.2% F18F (TTT→TTC)  cspI ← cold shock protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE980,2510AG13.2% 259.4 / 26.1 106F18F (TTT→TTC) cspIcold shock protein
Reads supporting (aligned to +/- strand):  ref base A (48/44);  new base G (10/4);  total (58/48)
Fisher's exact test for biased strand distribution p-value = 2.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01

Only 100 of 121 total aligned reads displayed.

AATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTG  >  minE/980181‑980319
                                                                      |                                                                    
aaTCGTTGCTCTGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCa                                                                      <  1:5691882/71‑1 (MQ=255)
 aTCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCaa                                                                     <  1:4837128/71‑1 (MQ=255)
       gCTCTGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCt                                                                <  1:3456392/70‑1 (MQ=255)
        ctctGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCttt                                                              >  1:4420352/1‑71 (MQ=255)
          ctGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCttttt                                                            >  1:104868/1‑71 (MQ=255)
           tGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCttttt                                                            >  1:128643/1‑70 (MQ=255)
           tGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCttttt                                                            >  1:1620991/1‑70 (MQ=255)
           tGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCATGATGAAACCaaa                                                                    <  1:5561120/62‑1 (MQ=255)
            gAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCaaa                                                                    >  1:4012266/1‑61 (MQ=255)
             aaTTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCttttt                                                            <  1:3675894/68‑1 (MQ=255)
             aaTTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCttttt                                                            <  1:1508089/68‑1 (MQ=255)
             aaTTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTc                                                           <  1:2949205/69‑1 (MQ=255)
               ttGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCa                                                          <  1:2919994/68‑1 (MQ=255)
               ttGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCa                                                          <  1:4550533/68‑1 (MQ=255)
               ttGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCa                                                          <  1:4578964/68‑1 (MQ=255)
                 gCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAAc                                                                  >  1:5680592/1‑58 (MQ=255)
                 gCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGt                                                      >  1:3491141/1‑70 (MQ=255)
                  cTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCttttt                                                            <  1:380875/63‑1 (MQ=255)
                  cTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCttttt                                                            <  1:5457685/63‑1 (MQ=255)
                  cTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTa                                                    <  1:3922659/71‑1 (MQ=255)
                  cTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCa                                                          >  1:2389092/1‑65 (MQ=255)
                   tGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCttttt                                                            <  1:3410184/62‑1 (MQ=255)
                    gagaAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTaaa                                                  >  1:1216871/1‑71 (MQ=255)
                      gaAATGGACAAACTCATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAAc                                                                  >  1:2788336/1‑53 (MQ=37)
                       aaaTGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCa                                               <  1:2167906/71‑1 (MQ=255)
                       aaaTAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAAc                                                 >  1:220450/1‑69 (MQ=255)
                         atggACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAtt                                             >  1:1039078/4‑71 (MQ=255)
                         atggACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAtt                                             >  1:1490823/4‑71 (MQ=255)
                          tggACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAttt                                            <  1:1379447/69‑1 (MQ=255)
                            gACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAg                                                         >  1:2381801/1‑56 (MQ=255)
                            caCAAACACATCTTTACTACCATCAACAGGAGAAATAAAGCCGAAACCTTTATCAGCGTTAAACAAtttta                                          >  1:1298922/2‑69 (MQ=255)
                             aCAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCaaa                                                                    >  1:3443239/1‑44 (MQ=255)
                             aCAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCaaa                                                                    >  1:4190745/1‑44 (MQ=255)
                             aCAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTc                                                           <  1:4785105/53‑1 (MQ=255)
                             aCAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTc                                                           <  1:4048922/53‑1 (MQ=255)
                             aCAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCa                                                          <  1:4158835/54‑1 (MQ=255)
                             aCAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCa                                                          <  1:395464/54‑1 (MQ=255)
                               aaaCACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAgg                                                        >  1:4306035/1‑54 (MQ=255)
                               aaaCACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTa                                       <  1:5510559/71‑1 (MQ=255)
                               aaaCACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTa                                       <  1:2315796/71‑1 (MQ=255)
                               aaaCACATCTTTACTACCATCAACAGGAGAAATAAAGCCGAAACCTTTATCAGCGTTAAACCATTTTACt                                        >  1:5127617/1‑70 (MQ=255)
                                 acacATCTTTAGAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTAcga                                       >  1:4621677/1‑67 (MQ=9)
                                 acacATCTTTAGAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTAcga                                       >  1:1052620/1‑67 (MQ=9)
                                  cacaTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAttt                                            >  1:1567424/1‑63 (MQ=255)
                                  cacaTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTc                                           >  1:1156252/1‑64 (MQ=255)
                                  cacaTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAAc                                    <  1:1513145/71‑1 (MQ=255)
                                    caTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAg                                                         >  1:631642/1‑48 (MQ=255)
                                    caTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAg                                                         >  1:5185732/1‑48 (MQ=255)
                                    caTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAg                                                         >  1:1659681/1‑48 (MQ=255)
                                       cTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCa                                  >  1:5511648/1‑68 (MQ=255)
                                        tttGCTGCCATCTTTCGGCGTGATGAAACCAAAAcc                                                                 >  1:1263479/1‑36 (MQ=255)
                                        tttGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAAc                                                 <  1:5616151/52‑1 (MQ=255)
                                        tttGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCa                              >  1:3707685/1‑71 (MQ=255)
                                         ttGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGt                                >  1:3644293/1‑68 (MQ=255)
                                         ttGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAt                             >  1:5221626/1‑71 (MQ=255)
                                          tgctgcCATCATCAGGAGTGATAAAGCCAAAACCTTTATCTGCGTTAAACCATTTTACTAAAc                                    >  1:4022803/1‑63 (MQ=9)
                                           gctgcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAtt                            <  1:4479250/70‑1 (MQ=255)
                                            gagcCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAttt                           <  1:2003423/68‑1 (MQ=255)
                                            gagcCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAttt                           <  1:82126/68‑1 (MQ=255)
                                            ctgcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAttt                           <  1:1591862/70‑1 (MQ=255)
                                              gcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAAc                                                 <  1:3448645/46‑1 (MQ=255)
                                               ccATCTTTGGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt                        >  1:2737445/1‑70 (MQ=255)
                                               ccATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt                        <  1:4351269/70‑1 (MQ=255)
                                                 aTCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTa                                       <  1:3092487/53‑1 (MQ=255)
                                                 aTCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTa                                       <  1:160521/53‑1 (MQ=255)
                                                    tttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTa                      >  1:5261761/1‑67 (MQ=255)
                                                    tttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACa                  >  1:1425652/1‑71 (MQ=255)
                                                     ttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACa                  >  1:2488971/1‑70 (MQ=255)
                                                      tCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACAt                 >  1:2333624/1‑70 (MQ=255)
                                                       cGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATaa               >  1:151545/1‑71 (MQ=255)
                                                         gagTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAtt                            >  1:4568935/3‑56 (MQ=255)
                                                           gTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAatta            >  1:2103233/1‑70 (MQ=255)
                                                            tgatgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCTCTAAACCAGTCATTTTGTTAGACATAatta            <  1:4387484/69‑1 (MQ=255)
                                                            tgataaAGCCAAAACCTTTATCTGCGTTAAACCAt                                              <  1:5295674/35‑1 (MQ=12)
                                                             gatgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAatta            <  1:3978299/68‑1 (MQ=255)
                                                              atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAttt                           <  1:3203337/52‑1 (MQ=255)
                                                              atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt                        <  1:5633836/55‑1 (MQ=255)
                                                              atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt                        <  1:4986770/55‑1 (MQ=255)
                                                              atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt                        <  1:210327/55‑1 (MQ=255)
                                                              atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt                        <  1:4753505/55‑1 (MQ=255)
                                                              atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt                        <  1:8492/55‑1 (MQ=255)
                                                               tgaAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAtt                            >  1:899380/1‑50 (MQ=255)
                                                               tgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAat              >  1:2128480/1‑64 (MQ=255)
                                                                gaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTg                         >  1:4645415/1‑52 (MQ=255)
                                                                gaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTg                         >  1:2865127/1‑52 (MQ=255)
                                                                gaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAatt             <  1:1845520/64‑1 (MQ=255)
                                                                gaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAatt             <  1:4098546/64‑1 (MQ=255)
                                                                 aaaCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAATCCAGTCATTTTGTTAGACATAATTATTAc        >  1:3841039/1‑68 (MQ=255)
                                                                 aaaCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCtt     >  1:923052/1‑71 (MQ=255)
                                                                  aaCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCttt    >  1:4390881/1‑71 (MQ=255)
                                                                    ccAAAACCTTTATCTGCGTTAAACCATTTTACTAAACCAGTCa                              >  1:1923790/1‑43 (MQ=25)
                                                                    ccAAAACCTTTATCTGCGTTAAACCATTTTACTAAACCAGTCa                              >  1:3179028/1‑43 (MQ=25)
                                                                     cAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAtttt                          >  1:5024372/1‑46 (MQ=255)
                                                                     cAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg  >  1:5017903/1‑70 (MQ=255)
                                                                      aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt                        >  1:5375720/1‑47 (MQ=255)
                                                                      aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg  >  1:5498046/1‑69 (MQ=255)
                                                                      aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg  >  1:2074788/1‑69 (MQ=255)
                                                                      aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg  >  1:490479/1‑69 (MQ=255)
                                                                      aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg  >  1:3169106/1‑69 (MQ=255)
                                                                      aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACAAGTCATTTTGt                        >  1:2076230/1‑47 (MQ=255)
                                                                      |                                                                    
AATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTG  >  minE/980181‑980319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑