| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 980,263 | A→T | 5.8% | P14P (CCT→CCA) ‡ | cspI ← | cold shock protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 980,263 | 0 | A | T | 5.8% | 337.4 / 8.8 | 119 | P14P (CCT→CCA) ‡ | cspI | cold shock protein |
| Reads supporting (aligned to +/- strand): ref base A (61/51); new base T (4/3); total (65/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 127 total aligned reads displayed.
AATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCT > minE/980194‑980333 | aaTTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAg < 1:4446119/71‑1 (MQ=255) ttGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCa < 1:5582488/68‑1 (MQ=255) ttGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCa < 1:4550533/68‑1 (MQ=255) ttGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCa < 1:4578964/68‑1 (MQ=255) gCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGt > 1:3491141/1‑70 (MQ=255) gCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGt > 1:3022718/1‑70 (MQ=255) cTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTa < 1:3922659/71‑1 (MQ=255) cTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCa > 1:2389092/1‑65 (MQ=255) gagaAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTaaa > 1:1216871/1‑71 (MQ=255) aaaTGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCa < 1:2167906/71‑1 (MQ=255) aaaTAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAAc > 1:220450/1‑69 (MQ=255) atggACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAtt > 1:1039078/4‑71 (MQ=255) atggACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAtt > 1:1490823/4‑71 (MQ=255) gACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAg > 1:2381801/1‑56 (MQ=255) caCAAACACATCTTTACTACCATCAACAGGAGAAATAAAGCCGAAACCTTTATCAGCGTTAAACCAtttta > 1:1453682/2‑69 (MQ=255) caCAAACACATCTTTACTACCATCAACAGGAGAAATAAAGCCGAAACCTTTATCAGCGTTAAACAAtttta > 1:1298922/2‑69 (MQ=255) aCAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCa < 1:395464/54‑1 (MQ=255) aCAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCa < 1:4158835/54‑1 (MQ=255) aaaCACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTa < 1:720516/71‑1 (MQ=255) aaaCACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTa < 1:5510559/71‑1 (MQ=255) aaaCACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTa < 1:2315796/71‑1 (MQ=255) aaaCACATCTTTACTACCATCAACAGGAGAAATAAAGCCGAAACCTTTATCAGCGTTAAACCATTTTACt > 1:5153911/1‑70 (MQ=255) acacATCTTTAGAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTAcga > 1:1052620/1‑67 (MQ=9) cacaTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAttt > 1:1567424/1‑63 (MQ=255) cacaTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAAc < 1:1513145/71‑1 (MQ=255) caTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAg > 1:5185732/1‑48 (MQ=255) caTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAg > 1:1659681/1‑48 (MQ=255) cTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCa > 1:5511648/1‑68 (MQ=255) tttGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAAc < 1:5616151/52‑1 (MQ=255) ttGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGt > 1:3644293/1‑68 (MQ=255) ttGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAt > 1:5221626/1‑71 (MQ=255) tgctgcCATCATCAGGAGTGATAAAGCCAAAACCTTTATCTGCGTTAAACCATTTTACTAAAc > 1:4022803/1‑63 (MQ=9) gctgcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAtt < 1:4479250/70‑1 (MQ=255) gctgcCATCATCAGGAGTGATAAAGCCAAAACCTTTATCTGCGTTAAACCATTTTACTAAACCAGTCAtt < 1:2100745/70‑1 (MQ=12) gagcCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAttt < 1:3892167/68‑1 (MQ=255) gagcCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAttt < 1:2003423/68‑1 (MQ=255) ctgcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAttt < 1:1591862/70‑1 (MQ=255) ccATCTTTGGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt > 1:2737445/1‑70 (MQ=255) ccATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt < 1:4351269/70‑1 (MQ=255) aTCTTTCGGTGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTa < 1:3328034/53‑1 (MQ=255) aTCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTa < 1:160521/53‑1 (MQ=255) aTCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTa < 1:3092487/53‑1 (MQ=255) tttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACa > 1:1425652/1‑71 (MQ=255) ttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACa > 1:4028751/1‑70 (MQ=255) gagTGATGAAGCCGAAGCCTTTGTCAGCGTTGAGCCATTTTACGATACCAGTCAtt > 1:3514997/3‑56 (MQ=255) gagTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAtt > 1:4568935/3‑56 (MQ=255) gagTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAtt > 1:1857598/3‑56 (MQ=255) gTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAatta > 1:2103233/1‑70 (MQ=255) tgatgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCTCTAAACCAGTCATTTTGTTAGACATAatta < 1:4387484/69‑1 (MQ=255) tgataaAGCCAAAACCTTTATCTGCGTTAAACCAt < 1:5295674/35‑1 (MQ=12) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAttt < 1:3203337/52‑1 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt < 1:5633836/55‑1 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt < 1:4986770/55‑1 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt < 1:4753505/55‑1 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt < 1:8492/55‑1 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt < 1:210327/55‑1 (MQ=255) tgaAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAtt > 1:899380/1‑50 (MQ=255) gaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTg > 1:2865127/1‑52 (MQ=255) gaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTg > 1:4645415/1‑52 (MQ=255) gaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAatt < 1:1845520/64‑1 (MQ=255) aaaCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAATCCAGTCATTTTGTTAGACATAATTATTAc > 1:3841039/1‑68 (MQ=255) aaaCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCtt > 1:923052/1‑71 (MQ=255) aaCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCttt > 1:4390881/1‑71 (MQ=255) ccAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg < 1:2256864/71‑1 (MQ=255) ccAAAACCTTTATCTGCGTTAAACCATTTTACTAAACCAGTc > 1:1237644/1‑42 (MQ=25) ccAAAACCTTTATCTGCGTTAAACCATTTTACTAAACCAGTCa > 1:3179028/1‑43 (MQ=25) ccAAAACCTTTATCTGCGTTAAACCATTTTACTAAACCAGTCa > 1:1923790/1‑43 (MQ=25) cAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAtttt > 1:5024372/1‑46 (MQ=255) cAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:2594071/1‑70 (MQ=255) cAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:5017903/1‑70 (MQ=255) aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt > 1:5375720/1‑47 (MQ=255) aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:5498046/1‑69 (MQ=255) aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:5448399/1‑69 (MQ=255) aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:2074788/1‑69 (MQ=255) aaaaCCTTTATCTGCGTTAAACCATTTTACTAAACCAGt < 1:4281685/39‑1 (MQ=25) aaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTa > 1:2629414/1‑48 (MQ=255) aaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:3407416/1‑68 (MQ=255) aaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:3166722/1‑68 (MQ=255) aaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTgaaga < 1:405913/71‑1 (MQ=255) aaCCTTTATCAGCGTTAAACCATTTTACTAAACCa > 1:1136215/1‑35 (MQ=25) aaCCTTTATCAGCGTTAAACCATTTTACTAAACCa > 1:1012348/1‑35 (MQ=25) aCCTTTTTCAGGGTTAAACCATTTCACTATACCAGTCATTTTGt < 1:5152605/44‑1 (MQ=255) aCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAattat > 1:4752905/1‑57 (MQ=255) aCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAattat > 1:2418489/1‑57 (MQ=255) aCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAattat > 1:4194263/1‑57 (MQ=255) aCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTgaagaa < 1:2837519/71‑1 (MQ=255) ccTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGaaa > 1:691397/1‑71 (MQ=255) tttgtCAGCGTTGAACCATTTTACGATACCAGTCAttt < 1:5566213/38‑1 (MQ=12) tttgtCAGCGTTGAACCATTTTACGATACCAGTCAttt < 1:2054396/38‑1 (MQ=12) tttgtCAGCGTTGAACCATTTTACGATACCAGTCAttt < 1:4874612/38‑1 (MQ=12) tttatCAGCGTTAAACCATTTTACTAAACCAGTCAttt < 1:2365632/38‑1 (MQ=25) tttatCAGCGTTAAACCATTTTACTAAACCAGTCATTTTATTTGa < 1:1796779/45‑1 (MQ=255) tttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAAtt < 1:2627508/69‑1 (MQ=255) tttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAAtt < 1:4040715/69‑1 (MQ=255) tttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTa < 1:5659966/70‑1 (MQ=255) tttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAg > 1:697425/1‑71 (MQ=255) tCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGc < 1:2869713/70‑1 (MQ=255) tCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGc < 1:1376651/70‑1 (MQ=255) tCAGCGTTAAACCATTTTACTAAACCAGTCATTTTAttt < 1:3098439/39‑2 (MQ=25) cAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGc < 1:778949/69‑1 (MQ=255) | AATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCT > minE/980194‑980333 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |