| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 980,281 | T→G | 8.5% | L8F (TTA→TTC) ‡ | cspI ← | cold shock protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 980,281 | 0 | T | G | 8.5% | 287.4 / 20.4 | 118 | L8F (TTA→TTC) ‡ | cspI | cold shock protein |
| Reads supporting (aligned to +/- strand): ref base T (53/55); new base G (4/6); total (57/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.46e-01 | |||||||||||
Only 100 of 122 total aligned reads displayed.
CAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGA > minE/980211‑980350 | aaaCACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTa < 1:5510559/71‑1 (MQ=255) aaaCACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTa < 1:720516/71‑1 (MQ=255) aaaCACATCTTTACTACCATCAACAGGAGAAATAAAGCCGAAACCTTTATCAGCGTTAAACCATTTTACt > 1:5153911/1‑70 (MQ=255) aaaCACATCTTTACTACCATCAACAGGAGAAATAAAGCCGAAACCTTTATCAGCGTTAAACCATTTTACt > 1:5127617/1‑70 (MQ=255) cacaTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAAc < 1:1513145/71‑1 (MQ=255) cTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCa > 1:5511648/1‑68 (MQ=255) ttGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGt > 1:3644293/1‑68 (MQ=255) tgctgcCATCATCAGGAGTGATAAAGCCAAAACCTTTATCTGCGTTAAACCATTTTACTAAAc > 1:4022803/1‑63 (MQ=9) gctgcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAtt < 1:4479250/70‑1 (MQ=255) gagcCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAttt < 1:3892167/68‑1 (MQ=255) gagcCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAttt < 1:82126/68‑1 (MQ=255) ccATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt < 1:4351269/70‑1 (MQ=255) aTCTTTCGGTGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTa < 1:3328034/53‑1 (MQ=255) aTCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTa < 1:160521/53‑1 (MQ=255) tttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTa > 1:5261761/1‑67 (MQ=255) tttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACa > 1:1425652/1‑71 (MQ=255) ttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACa > 1:2488971/1‑70 (MQ=255) ttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACa > 1:4028751/1‑70 (MQ=255) tCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACAt > 1:2333624/1‑70 (MQ=255) cGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATaa > 1:151545/1‑71 (MQ=255) gagTGATGAAGCCGAAGCCTTTGTCAGCGTTGAGCCATTTTACGATACCAGTCAtt > 1:3514997/3‑56 (MQ=255) gagTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAtt > 1:1857598/3‑56 (MQ=255) gagTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAtt > 1:4568935/3‑56 (MQ=255) gTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAatta > 1:2103233/1‑70 (MQ=255) tgatgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCTCTAAACCAGTCATTTTGTTAGACATAatta < 1:4387484/69‑1 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAttt < 1:3203337/52‑1 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt < 1:5633836/55‑1 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt < 1:8492/55‑1 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt < 1:4753505/55‑1 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt < 1:4986770/55‑1 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt < 1:210327/55‑1 (MQ=255) tgaAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAtt > 1:899380/1‑50 (MQ=255) tgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAat > 1:2128480/1‑64 (MQ=255) gaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTg > 1:4645415/1‑52 (MQ=255) gaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTg > 1:2865127/1‑52 (MQ=255) gaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAatt < 1:1845520/64‑1 (MQ=255) gaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAatt < 1:4098546/64‑1 (MQ=255) aaaCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAATCCAGTCATTTTGTTAGACATAATTATTAc > 1:3841039/1‑68 (MQ=255) aaaCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCtt > 1:923052/1‑71 (MQ=255) ccAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg < 1:2256864/71‑1 (MQ=255) ccAAAACCTTTATCTGCGTTAAACCATTTTACTAAACCAGTc > 1:1237644/1‑42 (MQ=25) ccAAAACCTTTATCTGCGTTAAACCATTTTACTAAACCAGTCa > 1:1923790/1‑43 (MQ=25) ccAAAACCTTTATCTGCGTTAAACCATTTTACTAAACCAGTCa > 1:3179028/1‑43 (MQ=25) cAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAtttt > 1:5024372/1‑46 (MQ=255) cAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:2594071/1‑70 (MQ=255) aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt > 1:5375720/1‑47 (MQ=255) aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:3169106/1‑69 (MQ=255) aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:490479/1‑69 (MQ=255) aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:5448399/1‑69 (MQ=255) aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:2074788/1‑69 (MQ=255) aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACAAGTCATTTTGt > 1:2076230/1‑47 (MQ=255) aaaaCCTTTATCTGCGTTAAACCATTTTACTAAACCAGt < 1:4281685/39‑1 (MQ=25) aaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTa > 1:2629414/1‑48 (MQ=255) aaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:3407416/1‑68 (MQ=255) aaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:3166722/1‑68 (MQ=255) aaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTgaaga < 1:405913/71‑1 (MQ=255) aaCCTTTATCAGCGTTAAACCATTTTACTAAACCa > 1:1012348/1‑35 (MQ=25) aaCCTTTATCAGCGTTAAACCATTTTACTAAACCa > 1:1136215/1‑35 (MQ=25) aCCTTTTTCAGGGTTAAACCATTTCACTATACCAGTCATTTTGt < 1:5152605/44‑1 (MQ=255) aCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAattat > 1:2418489/1‑57 (MQ=255) aCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTgaagaa < 1:5581304/71‑1 (MQ=255) aCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTgaagaa < 1:2837519/71‑1 (MQ=255) cTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAAt < 1:2764800/71‑1 (MQ=255) tttgtCAGCGTTGAACCATTTTACGATACCAGTCAttt < 1:5566213/38‑1 (MQ=12) tttgtCAGCGTTGAACCATTTTACGATACCAGTCAttt < 1:2054396/38‑1 (MQ=12) tttgtCAGCGTTGAACCATTTTACGATACCAGTCAttt < 1:4874612/38‑1 (MQ=12) tttatCAGCGTTAAACCATTTTACTAAACGAGTCAttt < 1:447067/38‑1 (MQ=21) tttatCAGCGTTAAACCATTTTACTAAACCAGTCAttt < 1:2365632/38‑1 (MQ=25) tttatCAGCGTTAAACCATTTTACTAAACCAGTCAttt < 1:5506022/38‑1 (MQ=25) tttatCAGCGTTAAACCATTTTACTAAACCAGTCATTTTATTTGa < 1:1796779/45‑1 (MQ=255) tttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAAtt < 1:2627508/69‑1 (MQ=255) tttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAAtt < 1:4040715/69‑1 (MQ=255) tttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTa < 1:5659966/70‑1 (MQ=255) tttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTa < 1:165492/70‑1 (MQ=255) tttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAg > 1:697425/1‑71 (MQ=255) tCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGc < 1:2869713/70‑1 (MQ=255) tCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGc < 1:1376651/70‑1 (MQ=255) tCAGCGTTAAACCATTTTACTAAACCAGTCATTTTAttt < 1:3098439/39‑2 (MQ=25) cAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGc < 1:778949/69‑1 (MQ=255) aGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCt < 1:1923509/71‑1 (MQ=255) ttAAACCATTTTACTAAACCAGTCATTTTATTTGa < 1:5405127/35‑1 (MQ=25) ttAAACCATTTTACTAAACCAGTCATTTTATTAGACAt < 1:26667/38‑1 (MQ=38) ttAAACCATTTTACTAAACCAGTCATTTTATTAGACAt < 1:3734753/38‑1 (MQ=38) ttAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAat < 1:2705755/41‑1 (MQ=255) aaaCCATTTTACTAAACCAGTCATTTTATTAGACATat > 1:208707/1‑37 (MQ=37) aaaCCATTTTACTAAACCAGTCATTTTATTAGACATAtttta > 1:4194764/1‑40 (MQ=37) aaCCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAg < 1:1135570/71‑1 (MQ=255) aaCCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAg < 1:5210032/71‑1 (MQ=255) aCCATTTCACTAAACCAGTCATTTTGTTAGACATAatta > 1:3903845/1‑39 (MQ=255) aCCATTTCACTAAACCAGTCATTTTGTTAGACATAatta > 1:3855462/1‑39 (MQ=255) aCCATTTCACTAAACCAGTCATTTTGTTAGACATAatta > 1:1228825/1‑39 (MQ=255) aCCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTg < 1:5656010/65‑1 (MQ=255) ccATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTg > 1:5586187/1‑64 (MQ=255) tttCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTgaagaa < 1:805918/50‑1 (MQ=255) tCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg < 1:930345/43‑1 (MQ=255) tCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGaaa > 1:1662990/1‑49 (MQ=255) cACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTcc > 1:2260131/1‑71 (MQ=255) cTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTg < 1:1574227/56‑1 (MQ=255) cTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGa < 1:1615037/71‑1 (MQ=255) tAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGa > 1:4667678/1‑70 (MQ=255) | CAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGA > minE/980211‑980350 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |