| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 982,670 | A→C | 16.2% | N39T (AAT→ACT) | ydfA → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 982,670 | 0 | A | C | 16.2% | 151.4 / 27.1 | 74 | N39T (AAT→ACT) | ydfA | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (25/37); new base C (4/8); total (29/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGATTTCGACACAATCATGGAAA > minE/982607‑982736 | cAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGcc > 1:4718072/1‑71 (MQ=255)cAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGcc > 1:578358/1‑71 (MQ=255)cAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGcc > 1:5012760/1‑71 (MQ=255)cAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGc < 1:1170512/70‑1 (MQ=255)cAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGc < 1:1448562/70‑1 (MQ=255) aaCCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGcgcg < 1:4637559/71‑1 (MQ=255) aaCCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGcgcg < 1:5188924/71‑1 (MQ=255) aaCCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCg > 1:4722547/1‑71 (MQ=255) aGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGCGCGCCga < 1:3936469/71‑2 (MQ=255) aGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGCGCGCCga < 1:3247907/71‑2 (MQ=255) aCGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCgctgg < 1:3627447/71‑1 (MQ=255) cGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGaaaa > 1:4481924/1‑54 (MQ=255) cGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATg > 1:670407/1‑56 (MQ=255) cACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAAt < 1:724862/52‑1 (MQ=255) aaCGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGCGCGCCGatggttgg < 1:3552788/70‑1 (MQ=255) cGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGCGCGCCgatggttg > 1:5157372/1‑68 (MQ=255) cGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGCGCGCCgatggttg > 1:3223200/1‑68 (MQ=255) cGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAAATGGTGCGCGCCgatggttg > 1:3871693/1‑68 (MQ=255) cGTTCACCGCAATGACGTATACCAATAGCAAAACGTTTAAAACCGAAAATGGTGcc < 1:8980/56‑1 (MQ=255) cACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGCGCGCCGATGGTTGgag > 1:5012133/1‑66 (MQ=255) cACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaaga < 1:4180364/70‑1 (MQ=255) cACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaaga < 1:2573547/70‑1 (MQ=255) cACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaaga < 1:4847846/70‑1 (MQ=255) ctcAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACt > 1:2670830/3‑71 (MQ=255) cGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTg < 1:3073809/47‑1 (MQ=255) ccaTGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTg > 1:5532609/3‑42 (MQ=37) aTGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaa < 1:1546652/61‑1 (MQ=255) aTGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaa < 1:5006571/61‑1 (MQ=255) tGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAAt < 1:2734298/38‑1 (MQ=255) tGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAAt < 1:5265494/38‑1 (MQ=255) tGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGgt < 1:2549657/71‑1 (MQ=255) tGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGgt < 1:3351675/71‑1 (MQ=255) gACGTATACCAAAAGCAAAACGTTTAAAACCGaaaa > 1:2678744/1‑36 (MQ=255) gACGTATACCAAAAGCAAAACGTTTAAAACCGaaaa > 1:5386829/1‑36 (MQ=255) aCGTATACCAAAAGCAAAACGTTTAAAACCGaaaa > 1:811884/1‑35 (MQ=255) aCGTATACCAAAAGCAAAACGTTTAAAACCGaaaa > 1:4665001/1‑35 (MQ=255) aCGTATACCAAAAGCAAAACGTTTAAAACCGAAAATgg > 1:2599585/1‑38 (MQ=255) taCCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTc < 1:5663943/41‑1 (MQ=255) taCCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTc < 1:5434580/41‑1 (MQ=255) taCCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTc < 1:3813525/41‑1 (MQ=255) taCCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAg < 1:1387796/62‑1 (MQ=255) ccAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGgtgagtga < 1:3495523/68‑1 (MQ=255) ccAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGatat > 1:2952438/1‑71 (MQ=255) aaaaGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATg > 1:1078776/1‑70 (MQ=255) aaaGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGa > 1:3235177/1‑71 (MQ=255) aaGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaa < 1:1216691/46‑1 (MQ=255) aaGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaa < 1:4209019/46‑1 (MQ=255) aaGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGAt > 1:4786817/1‑71 (MQ=255) aaGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGAt > 1:3058826/1‑71 (MQ=255) gCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGgtgag > 1:4030641/1‑59 (MQ=255) gCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGgtgag > 1:824947/1‑59 (MQ=255) gCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGgtgag > 1:2541855/1‑59 (MQ=255) gCAAAACATTTAAAACCGAAACTGGTGCGCGCCgatgg < 1:5535686/38‑1 (MQ=16) gCAAAACATTTAAAACCGAAACTGGTGCGCGCCgatgg < 1:138582/38‑1 (MQ=16) gCAAAACATTTAAAACCGAAACTGGTGCGCGCCgatgg < 1:904566/38‑1 (MQ=16) cAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGATTTc > 1:3982701/1‑71 (MQ=255) aaaaCGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGatat < 1:1819954/63‑1 (MQ=255) aaaaCGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGatat < 1:1281384/63‑1 (MQ=255) aaaCGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTc < 1:4594842/49‑1 (MQ=255) aaaCGTTTAAAACCGAAAATGGTGCCCGGCgctggctg < 1:5362432/38‑1 (MQ=255) aaCGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaagaaa > 1:1557849/1‑45 (MQ=255) aaCGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaaga < 1:4373710/43‑1 (MQ=255) aCGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGga > 1:848033/1‑38 (MQ=255) cGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGATTTc < 1:2853523/66‑1 (MQ=255) gTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGATTTCGacac > 1:1360133/1‑70 (MQ=255) tttAAAACCGAAAATGGTGCCCGTCGCTGTCTGga < 1:1823361/35‑1 (MQ=255) tttAAAACCGAAAATGGTGCCCGTCGCTGGCTGga < 1:3863878/35‑1 (MQ=255) tttAAAACCGAAAATGGTGCCCGTCGCTGGCTGga < 1:52607/35‑1 (MQ=255) tttAAAACCGAAAATGGTGCCCGTCGCTGGCTGga < 1:4712045/35‑1 (MQ=255) tttAAAACCGAAAATGGTGCCCGTCGCTGGCTGga < 1:462759/35‑1 (MQ=255) ttAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGATTTCGACACAAt > 1:231596/1‑71 (MQ=255) tAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaagaaa < 1:2010744/39‑1 (MQ=255) aaaaCCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAgg > 1:2804462/1‑44 (MQ=255) aaaCCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGATTTCGACACAATCa < 1:2700682/70‑1 (MQ=255) aCCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAgg > 1:4197133/1‑41 (MQ=255) gAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTc > 1:3943956/1‑35 (MQ=255) gAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAg > 1:3515714/1‑37 (MQ=255) gAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGATTTCGACACAATCATGGaaa < 1:1496753/71‑1 (MQ=255) gAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGATTTCGACACAATCATGGa < 1:4864487/69‑1 (MQ=255) aaaaTGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGgt < 1:3779239/38‑1 (MQ=255) aaaaTGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGgt < 1:3168484/38‑1 (MQ=255) aaTGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGata > 1:2644295/1‑44 (MQ=255) aTGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGgtgag < 1:2108554/38‑1 (MQ=255) aTGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGgtgag < 1:4265683/38‑1 (MQ=255) | CAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGATTTCGACACAATCATGGAAA > minE/982607‑982736 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |