| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 983,505 | T→C | 23.6% | intergenic (+417/‑151) | ydfC → / → dicB | hypothetical protein/cell division inhibition protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 983,505 | 0 | T | C | 23.6% | 94.7 / 27.1 | 55 | intergenic (+417/‑151) | ydfC/dicB | hypothetical protein/cell division inhibition protein |
| Reads supporting (aligned to +/- strand): ref base T (24/18); new base C (7/6); total (31/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGACGTTTGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCT > minE/983435‑983568 | gTGACGTTTGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGAtgt > 1:3649234/1‑71 (MQ=255) gACGTTTGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATgtgg > 1:1174929/1‑71 (MQ=255) cGTTTGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATgtggtg < 1:3495389/71‑1 (MQ=255) cGTTTGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATgtggtg < 1:1767904/71‑1 (MQ=255) cGTTTGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATgtggt > 1:176120/1‑70 (MQ=255) tttGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGaa < 1:3659306/71‑1 (MQ=255) ttGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAAt > 1:4636548/1‑71 (MQ=255) tGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAAt < 1:4825560/70‑1 (MQ=255) tGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATg > 1:1136924/1‑71 (MQ=255) gCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGc < 1:4054426/70‑1 (MQ=255) tCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAg > 1:3054534/1‑71 (MQ=255) cAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAgc > 1:1048103/1‑71 (MQ=255) cAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAgc > 1:3741604/1‑71 (MQ=255) ttttACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATgtgg < 1:3132195/53‑1 (MQ=255) ttACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGAtgt > 1:598682/1‑49 (MQ=255) aCTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCAcgc < 1:3643948/70‑1 (MQ=255) cGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGaa > 1:3093459/1‑70 (MQ=255) tGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATgtggtg < 1:974370/45‑1 (MQ=255) tGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATgtggtg < 1:1066898/45‑1 (MQ=255) tGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAg > 1:3683709/1‑71 (MQ=255) aCTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGt > 1:3041768/1‑70 (MQ=255) tGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCg < 1:4819895/47‑1 (MQ=255) ctctGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTaaaa > 1:44310/1‑71 (MQ=255) ctGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAAc > 1:3472261/1‑70 (MQ=255) tGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGaa > 1:2422739/1‑38 (MQ=255) tGCCGCCCTTTTTAAAGTGAATTTTGGGATGTGGTGaa > 1:5619713/1‑38 (MQ=255) ccgccCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTg < 1:3661696/44‑1 (MQ=255) ccgccCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaa < 1:701072/71‑1 (MQ=255) gccCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaaaa > 1:2859085/1‑71 (MQ=255) gccCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaaaa > 1:3810154/1‑71 (MQ=255) cccTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaaaa > 1:3801619/1‑70 (MQ=255) cccTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaa < 1:2318810/67‑1 (MQ=255) cccTTTTTAAAGTGAATTTTGTGATGCGGTGAATGCGGCTAAGCGCAcg > 1:209816/1‑49 (MQ=38) cccTTTTTAAAGTGAATTTTGTGATGCGGTGAATGCGGCTAAGCGCAcg > 1:5018031/1‑49 (MQ=38) cccTTTTTAAAGTGAATTTTGTGATGCGGTGAATGCGGCTAAGCGCAcg > 1:4553311/1‑49 (MQ=38) ccTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAg > 1:2177498/1‑56 (MQ=255) tttttAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCgg < 1:1431139/49‑1 (MQ=255) tttttAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCgg < 1:2351362/49‑1 (MQ=255) tttttAAAGTGAATTTTGTGATGCGGTGAATGCGGCta < 1:1015920/38‑2 (MQ=38) tttAAAGTGAATTTTGTGATGCGGTGAATGCGGCTAAg > 1:5355539/1‑38 (MQ=37) tttAAAGTGAATTTTGTGATGCGGTGAATGCGGCTAAGCGCACGTggca > 1:2288969/1‑47 (MQ=37) ttAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaaa < 1:1153227/63‑1 (MQ=255) ttAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAgtgt < 1:3432727/70‑1 (MQ=255) ttAAAGAGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAgtgt > 1:60501/1‑70 (MQ=255) tAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGaa > 1:3522728/1‑47 (MQ=255) tAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGaa > 1:4790084/1‑47 (MQ=255) tAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGaa > 1:2777883/1‑47 (MQ=255) tAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGtt < 1:4752168/70‑1 (MQ=255) aaaGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAAc > 1:4377844/1‑63 (MQ=255) aaaGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAAc > 1:5115125/1‑63 (MQ=255) aaGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTAt < 1:1607623/70‑1 (MQ=255) tGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCAcg < 1:3577234/37‑1 (MQ=255) tGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCAcg < 1:4286088/37‑1 (MQ=255) tGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCAcg < 1:4858309/37‑1 (MQ=255) gAATTTTGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaaaa > 1:4340373/1‑50 (MQ=255) gAATTTTGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaaaa > 1:1109611/1‑50 (MQ=255) aaTTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTg > 1:1791464/1‑70 (MQ=255) ttttGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaa < 1:1661474/45‑1 (MQ=25) ttttGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaa < 1:5535740/45‑1 (MQ=25) ttttGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaa < 1:3726905/45‑1 (MQ=25) ttGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGAtt > 1:5099483/1‑70 (MQ=255) gtgATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATtct < 1:3279245/70‑1 (MQ=255) gATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaa < 1:4210935/39‑1 (MQ=25) gATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaa < 1:902769/39‑1 (MQ=25) tggtgAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGtt < 1:1989506/51‑1 (MQ=255) tggtgAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGtt < 1:4610845/51‑1 (MQ=255) | GTGACGTTTGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCT > minE/983435‑983568 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |