| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,014,482 | A→G | 20.0% | D529D (GAT→GAC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,014,482 | 0 | A | G | 20.0% | 119.3 / 24.3 | 60 | D529D (GAT→GAC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (29/19); new base G (7/5); total (36/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
CTGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCC > minE/1014418‑1014544 | ctGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTatcatc > 1:5478054/1‑70 (MQ=255)ctGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTatc < 1:5480882/67‑1 (MQ=255) tGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTat < 1:200366/65‑1 (MQ=255) tGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTat < 1:641805/65‑1 (MQ=255) gTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTatcat > 1:3974054/1‑67 (MQ=255) gTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTatcat > 1:1669720/1‑67 (MQ=255) tttATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAATAAGTCATTTCCTTTATCATCcac > 1:4075677/1‑70 (MQ=255) ttATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCcac < 1:4162374/69‑1 (MQ=255) ttATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCcac > 1:1964758/1‑69 (MQ=255) tATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCAGAAg > 1:651327/1‑71 (MQ=255) tATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAg > 1:3445596/1‑71 (MQ=255) tATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAg > 1:5554675/1‑71 (MQ=255) aTTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAg > 1:1624172/1‑70 (MQ=255) tCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTat < 1:4830794/57‑1 (MQ=255) tCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTat < 1:2043783/57‑1 (MQ=255) acaGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATaa > 1:1598061/1‑68 (MQ=255) acaGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATaa > 1:4695406/1‑68 (MQ=255) acaGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATaa > 1:5647785/1‑68 (MQ=255) cGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCgg < 1:1554401/71‑1 (MQ=255) tGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCcac < 1:4179032/52‑1 (MQ=255) gCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTatcat > 1:236799/1‑47 (MQ=255) aTTGTGTGAGTTGTATCTGGTGGAAGATGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCt > 1:3113571/1‑71 (MQ=255) ttGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAgg > 1:1743950/1‑52 (MQ=255) ttGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATa < 1:1742119/55‑1 (MQ=255) ttGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATc < 1:1179722/69‑1 (MQ=255) ttGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATc < 1:4372053/69‑1 (MQ=255) tgtgtgAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAAt > 1:1728731/1‑67 (MQ=255) tgtgtgAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAAt > 1:1273370/1‑67 (MQ=255) tgtgtgAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCtt > 1:5460216/1‑70 (MQ=255) tgtgtgAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTc > 1:3988852/1‑71 (MQ=255) tgtgtgAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTc > 1:4682293/1‑71 (MQ=255) gtgtgAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTc > 1:3169026/1‑70 (MQ=255) tgtgAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATa < 1:3197063/52‑1 (MQ=255) tgtgAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATa < 1:5010572/52‑1 (MQ=255) tgtgAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATa < 1:4757560/52‑1 (MQ=255) tgtgAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATa < 1:3759953/52‑1 (MQ=255) tgtgAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATa < 1:1142094/52‑1 (MQ=255) tgtgAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTcc > 1:5068504/1‑70 (MQ=255) gtgAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTatcat > 1:1972261/1‑40 (MQ=255) gtgAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTatcat > 1:3816463/1‑40 (MQ=255) gtgAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTatcat > 1:3516100/1‑40 (MQ=255) gtgAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCAc > 1:4427468/1‑71 (MQ=255) gAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATa < 1:1465795/49‑1 (MQ=255) gAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATa < 1:1792050/49‑1 (MQ=255) aGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATa < 1:813566/48‑1 (MQ=255) tGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAAt > 1:4483828/1‑58 (MQ=255) tGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAAt > 1:2839048/1‑71 (MQ=255) gTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAAt > 1:565999/1‑70 (MQ=255) aTCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAAtttt > 1:535536/1‑71 (MQ=255) tttgctgGAAGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATc > 1:5242002/3‑55 (MQ=25) ttgctgGAAGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGc > 1:3080222/2‑45 (MQ=19) ttgctgGAAGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTc > 1:443686/2‑57 (MQ=25) ttgctgGAAGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTc > 1:3661546/2‑57 (MQ=25) ctgGAAGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAg < 1:3389495/70‑1 (MQ=255) gaagaaGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGa > 1:4708865/1‑68 (MQ=255) gaagaaGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGAtag > 1:4840571/1‑69 (MQ=255) gaagaaGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATg > 1:1281765/1‑70 (MQ=255) gaagaaGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATg < 1:3396548/70‑1 (MQ=255) caagaagTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGAtag > 1:4840576/2‑69 (MQ=255) aagTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTc > 1:3585077/1‑71 (MQ=255) agTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTc < 1:1851522/70‑1 (MQ=255) tCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCAt > 1:1554285/1‑71 (MQ=255) cATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCAtt > 1:3199116/1‑71 (MQ=255) aTTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAAtt < 1:3480619/52‑1 (MQ=255) aTTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGAt > 1:5655099/1‑60 (MQ=255) tttCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTc < 1:1187718/41‑1 (MQ=255) tttCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTc < 1:3929580/41‑1 (MQ=255) tttCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTcc > 1:489388/1‑71 (MQ=255) ttCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTc < 1:4017569/40‑1 (MQ=255) taccTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTc < 1:384221/51‑1 (MQ=25) cTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTcga < 1:4102925/45‑3 (MQ=21) tttGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCAt < 1:5383765/63‑1 (MQ=255) | CTGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCC > minE/1014418‑1014544 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |