| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,014,542 | T→A | 25.3% | G509G (GGA→GGT) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,014,542 | 0 | T | A | 25.3% | 132.3 / 43.2 | 71 | G509G (GGA→GGT) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base T (30/23); new base A (9/9); total (39/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGCCGGA > minE/1014472‑1014608 | cATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCAtt > 1:3199116/1‑71 (MQ=255) tttCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTcc > 1:489388/1‑71 (MQ=255) ttgtcatcGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTc > 1:1254032/4‑71 (MQ=255) atcGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGa > 1:2639127/1‑70 (MQ=255) atcGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGa > 1:5086069/1‑70 (MQ=255) ccACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTccc > 1:2747386/1‑59 (MQ=255) cacaAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGatat > 1:1079912/1‑70 (MQ=255) cacaAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGatat > 1:1175426/1‑70 (MQ=255) cacaAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATAtt > 1:453674/1‑71 (MQ=255) acaAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTcc < 1:2981343/56‑1 (MQ=255) aaGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTc > 1:5501114/1‑69 (MQ=255) tAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCa > 1:126505/1‑51 (MQ=255) tAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCa > 1:119644/1‑51 (MQ=255) tAAACGCCGGGAAATCTTCTACTTCGATTTTCAAGATAGCTTCCATACCCAGCTCCGGa > 1:275311/1‑59 (MQ=255) tAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCg < 1:1877283/57‑1 (MQ=255) tAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAACACAt > 1:2076490/1‑71 (MQ=255) aaCGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCaa < 1:10312/64‑1 (MQ=255) aCGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATAcc > 1:4521493/1‑46 (MQ=255) cGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAACACATTCCa < 1:4032262/71‑1 (MQ=255) cGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAACACATTCCa < 1:4026277/71‑1 (MQ=255) cGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAACACATTCCa < 1:358922/71‑1 (MQ=255) gCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAg > 1:4561960/1‑47 (MQ=255) cGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAgat < 1:1093234/71‑3 (MQ=255) gggAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGct < 1:2595090/71‑1 (MQ=255) ggAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAgc < 1:450747/44‑2 (MQ=255) ggAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAgc < 1:3154114/44‑2 (MQ=255) ggAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAgc < 1:878322/44‑2 (MQ=255) ggAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCg < 1:3488393/48‑1 (MQ=255) ggAAATCTTCTACTTCGATTTTCCAGATAGATTCCATACCCAGCTCCGGATAAGCGACGCACTCCAgatg > 1:5477308/1‑67 (MQ=255) aaaTCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTc < 1:1354722/44‑1 (MQ=255) aaaTCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTc < 1:4764458/44‑1 (MQ=255) aaaTCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCtt < 1:1585056/71‑1 (MQ=255) cTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATa > 1:5071271/1‑71 (MQ=255) ttCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTcc < 1:4732056/40‑1 (MQ=255) ttCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTcc < 1:2219373/40‑1 (MQ=255) ttCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTc < 1:351022/48‑1 (MQ=255) ttCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTc < 1:1886663/48‑1 (MQ=255) ttCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATa > 1:3467154/1‑70 (MQ=255) cACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAgg > 1:5163477/1‑58 (MQ=255) cACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATa > 1:3412052/1‑67 (MQ=255) cACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATa > 1:388147/1‑67 (MQ=255) cACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACtt < 1:4335428/70‑1 (MQ=255) cACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATAATCAACACATTCCAGGCTCTTAATACtt < 1:2827364/70‑1 (MQ=255) cACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCAGGATATTCAACACATTCCAgg > 1:3877106/1‑58 (MQ=255) cACTTCAATTTTCCAGATGGCTTCCACTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTc > 1:5353610/1‑71 (MQ=255) aCTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTGAACACATTCCAGGCTCTTAATACTTcc > 1:5670052/1‑71 (MQ=255) aCTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTcc > 1:3431314/1‑71 (MQ=255) cTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGat < 1:3316729/39‑1 (MQ=25) cTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGat < 1:5003720/39‑1 (MQ=25) cTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGc < 1:499907/57‑1 (MQ=255) ttCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAgg > 1:1280102/1‑55 (MQ=255) cAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCaa > 1:3713759/1‑42 (MQ=255) cAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAgg > 1:1895583/1‑53 (MQ=255) cAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAg > 1:4543578/1‑52 (MQ=255) ttttCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAACACATTCCa < 1:2060825/48‑1 (MQ=255) ttttCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAACACATTCCa < 1:3468184/48‑1 (MQ=255) ttttCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATa > 1:4637325/1‑59 (MQ=255) ttttCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTgcgc > 1:4608584/1‑70 (MQ=255) tttCCAGATGGCTTCCATTCCCAGTTCCGGATATTc < 1:5380084/36‑1 (MQ=255) ttCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACtt > 1:5137394/1‑60 (MQ=255) ttCCAGAT‑GCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCaa < 1:839897/70‑1 (MQ=255) cAGATGGCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATa < 1:4875235/70‑1 (MQ=255) aTGGCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTaa > 1:1433669/1‑49 (MQ=255) gCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGc > 1:838174/1‑71 (MQ=255) gCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGc > 1:2413168/1‑71 (MQ=255) gCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGc < 1:3358408/71‑1 (MQ=255) cTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGcc < 1:1383428/71‑1 (MQ=255) cTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGcc < 1:1576348/71‑1 (MQ=255) cTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGcc > 1:836335/1‑71 (MQ=255) tCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGc > 1:4193030/1‑65 (MQ=255) ccATTCCCAGTTCCGGATATTCAACACATTCCAgg > 1:652606/1‑35 (MQ=255) ccATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGCCGGa > 1:3342986/1‑71 (MQ=255) ttCCCAGTTCCGGATATTCAACACATTCCAGGCTCtt < 1:381481/37‑1 (MQ=255) ttCCCAGTTCCGGATATTCAACACATTCCAGGCTCtt < 1:716008/37‑1 (MQ=255) ttCCCAGTTCCGGATATTCAACACATTCCAGGCTCtt < 1:1178778/37‑1 (MQ=255) | CATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGCCGGA > minE/1014472‑1014608 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |