| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,014,698 | A→G | 19.3% | S457S (AGT→AGC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,014,698 | 0 | A | G | 19.3% | 167.5 / 33.7 | 83 | S457S (AGT→AGC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (30/37); new base G (9/7); total (39/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAG > minE/1014630‑1014765 | aaGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCt < 1:530231/71‑1 (MQ=255) cgccgTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCg > 1:4858543/1‑71 (MQ=255) cgccgTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCg < 1:4932291/71‑1 (MQ=255) cgccgTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCg < 1:5149872/71‑1 (MQ=255) tttttACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTgcgc > 1:3348219/1‑71 (MQ=255) tACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTg > 1:118167/1‑70 (MQ=255) tACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTg < 1:2412482/70‑1 (MQ=255) cAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGc < 1:3500963/69‑1 (MQ=255) cAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCa < 1:1257073/70‑1 (MQ=255) cATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGa > 1:1866316/1‑71 (MQ=255) aTCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGAt > 1:785037/1‑71 (MQ=255) cGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATAc > 1:3708461/1‑44 (MQ=255) cGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATAc > 1:3604764/1‑44 (MQ=255) gTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCg < 1:5604358/48‑1 (MQ=255) gTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCg < 1:5680458/48‑1 (MQ=255) gTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCa > 1:72032/1‑61 (MQ=255) aCCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACAt > 1:2767763/1‑70 (MQ=255) gctggctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGaa > 1:3249287/1‑71 (MQ=255) gctgactgCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGag > 1:472056/1‑70 (MQ=255) ctggctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTg < 1:349343/57‑1 (MQ=255) ctggctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGa < 1:3328560/69‑1 (MQ=255) ctggctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCAACATAAGa < 1:2458362/69‑1 (MQ=255) ctgactgCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTa > 1:5578970/1‑66 (MQ=255) ggctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGc > 1:538187/1‑50 (MQ=255) gactgCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAgtc > 1:2740101/3‑70 (MQ=255) ctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatccat > 1:919753/1‑71 (MQ=255) ctgCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAgtccat < 1:635382/71‑1 (MQ=255) tgCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCAcgt < 1:5649444/60‑3 (MQ=255) tgCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAgtccata > 1:33357/1‑70 (MQ=255) gCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatccatcc > 1:4892227/1‑71 (MQ=255) gTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatcca < 1:3370554/65‑1 (MQ=255) ttGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAg < 1:1075610/44‑1 (MQ=255) ttGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAg < 1:5237625/44‑1 (MQ=255) ttGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAg < 1:4838559/44‑1 (MQ=255) ttGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAg < 1:4813594/44‑1 (MQ=255) ttGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAg < 1:469002/44‑1 (MQ=255) ttGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCAtccgtcc > 1:2943055/1‑71 (MQ=255) ttGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCATGCAg < 1:4093900/44‑1 (MQ=255) taccTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCAtacggcct > 1:2604965/3‑70 (MQ=255) tGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCAtccgtccg > 1:2437893/1‑71 (MQ=255) gCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCAtccgtccg > 1:153873/1‑70 (MQ=255) gCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCAtccgt < 1:4345819/67‑1 (MQ=255) accTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCAtacggcctg > 1:3405713/2‑69 (MQ=255) accTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCAtacggcctg > 1:5384115/2‑69 (MQ=255) accTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCAtacggcctg > 1:3270859/2‑69 (MQ=255) accTTTCGCCAGCATGATCATGCCGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCAtacggcct > 1:5142767/2‑69 (MQ=255) cTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCcggcg > 1:2273606/1‑71 (MQ=255) tttCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAgtcca < 1:4624087/59‑1 (MQ=255) ttCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGt > 1:3373890/1‑71 (MQ=255) tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACa > 1:1007613/1‑47 (MQ=255) tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACa > 1:2347324/1‑47 (MQ=255) tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGt < 1:1482870/70‑1 (MQ=255) tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTc > 1:4617686/1‑71 (MQ=255) gCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCggtggt < 1:208187/71‑1 (MQ=255) gCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGtg < 1:2335407/69‑2 (MQ=255) gCCAGCATGATCATACTTCCGCCCTGCGCTAGCAGTTGATCGACATAAGaa > 1:179943/1‑51 (MQ=255) ccAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGtt < 1:5185377/71‑1 (MQ=255) ccAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatc < 1:3114174/52‑1 (MQ=255) ccAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCAtccg < 1:5585939/58‑1 (MQ=255) ccAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt < 1:3198675/70‑1 (MQ=255) aGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTgg > 1:1936462/1‑71 (MQ=255) aGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTgg < 1:3163240/71‑1 (MQ=255) cATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt < 1:5507567/66‑1 (MQ=255) cATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGc > 1:2295277/1‑71 (MQ=255) tgttCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCa < 1:3217070/68‑1 (MQ=255) tGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATa < 1:469015/40‑1 (MQ=255) tGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATa < 1:2032453/40‑1 (MQ=255) tGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATa < 1:4895398/40‑1 (MQ=255) tGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTg > 1:1444583/1‑66 (MQ=255) tGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGcc < 1:4603298/70‑1 (MQ=255) gATCATACTTCCGCCCTGTGCTTGCAGTTGATCGACATAAGaa < 1:1828306/43‑1 (MQ=255) gATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGtt < 1:2871881/64‑1 (MQ=255) aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatccatcc > 1:3933305/1‑49 (MQ=255) aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatccatcc > 1:2968097/1‑49 (MQ=255) aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAat < 1:3905659/43‑1 (MQ=255) aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAat < 1:430467/43‑1 (MQ=255) aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAat < 1:4334084/43‑1 (MQ=255) aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGcc > 1:520481/1‑68 (MQ=255) tCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAg < 1:5372804/39‑1 (MQ=255) tCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAg < 1:1640576/39‑1 (MQ=255) tCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAg < 1:1201293/39‑1 (MQ=255) tCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCg > 1:4616898/1‑60 (MQ=255) aTACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAagag > 1:5288592/1‑70 (MQ=255) aCTTCCGCCCTGCGCTTGCAGTTGATCGACATAAg > 1:276670/1‑35 (MQ=255) aCTTCCGCCCTGCGCTTGCAGTTGATCGACATAAg > 1:917123/1‑35 (MQ=255) | AAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAG > minE/1014630‑1014765 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |